Enhanced antibody response in Atlantic salmon (Salmo salar L.) to Aeromonas salmonicida cell wall antigens using a bacterin containing β-1,3-M-Glucan as adjuvant

Aakre, Ragnhild; Wergeland, Heidrun I.; Aasjord, Per; Endresen, Curt

doi:10.1006/fsim.1994.1005

Cited by 64 publications

(42 citation statements)

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“…Following proteinase K digestion, reactivity was diminished; however, a fine banding pattern typical of the repeating units of the LPS O-polysaccharide chains was apparent. LPS is known to be immunogenic in Gram-negative fish pathogens such as Aeromonas salmonicida (Hastings & Ellis 1990, Lund et al 1991, Aakre et al 1994 and Vibrio species (Bøgwald et al Steine et al 2001). Although A. salmonicida LPS is immunogenic, it does not appear to play a protective role (Thuvander et al 1993, Aakre et al 1994.…”

Section: Discussionmentioning

confidence: 99%

“…LPS is known to be immunogenic in Gram-negative fish pathogens such as Aeromonas salmonicida (Hastings & Ellis 1990, Lund et al 1991, Aakre et al 1994 and Vibrio species (Bøgwald et al Steine et al 2001). Although A. salmonicida LPS is immunogenic, it does not appear to play a protective role (Thuvander et al 1993, Aakre et al 1994. Immunization with LPS from other Gram-negative bacteria has resulted in protection (Aoki et al 1984, Salati & Kusuda 1985, Saeed & Plumb 1986, Baba et al 1988, Velji et al 1990, Soltani & Kalbassi 2001, and this protection has been reported as antibody-mediated (Saeed & Plumb 1986, Velji et al 1991.…”

Section: Discussionmentioning

confidence: 99%

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Protective immunity in rainbow trout Oncorhynchus mykiss following immunization with distinct molecular mass fractions isolated from Flavobacterium psychrophilum

LaFrentz

LaPatra²,

Jones³

et al. 2004

Dis. Aquat. Org.

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Vaccine development for coldwater disease (CWD), also known as rainbow trout fry syndrome (RTFS), has been based primarily on whole-cell bacterins or outer membrane fractions of Flavobacterium psychrophilum. In the present study, immunogenic regions of the bacterium corresponding to 18-28, 41-49, and 70-100 kDa were identified by western blot analysis using rainbow trout Oncorhynchus mykiss immune sera. Following sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), antigens within these regions were isolated by electro-elution and used in immunization trials. Groups of rainbow trout fry were immunized with these regions emulsified with Freund's complete adjuvant (FCA) and a formalin-killed bacterin emulsified with FCA. It was demonstrated that the 70-100 and 41-49 kDa regions and F. psychrophilum treatments elicited significant protection when compared to the saline control following subcutaneous challenge with 2 doses of a virulent strain of F. psychrophilum. Immunization with the 70-100 kDa region resulted in near complete protection in fish with mean cumulative percent mortality (CPM) of 6% and mean relative percent survival (RPS) of 94% at the lower challenge dose (6.25 × 10 6 colony forming units fish -1

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protective immunity in rainbow trout Oncorhynchus mykiss following immunization with distinct molecular mass fractions isolated from Flavobacterium psychrophilum

LaFrentz

LaPatra²,

Jones³

et al. 2004

Dis. Aquat. Org.

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“…In addition, IP challenge involves the systemic immune response and may mask local protection (Vigneulle 1991) Furthermore, the use of P-glucan as an adjuvant showed no effect on the mortality rate compared to that obtained after a single vaccination. The study of Aakre et al (1994) in Atlantic salmon vaccinated against furunculosis produced contradictory results; they obtained a higher survival rate with glucanc0ntainin.g vaccine compared to a single bacterin vaccine, when fish were challenged 7 wk after vaccination. On the other hand, a challenge performed 12 wk after a booster vaccination showed no difference in protection between the glucan-adjuvanted and nonadjuvanted vaccine.…”

Section: Discussionmentioning

confidence: 99%

Effect of long-term oral administration of beta-glucan as an immunostimulant or an adjuvant on some non-specific parameters of the immune response of turbot Scophthalmus maximus

Baulny¹,

Quentel²,

Fournier³

et al. 1996

Dis. Aquat. Org.

125

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A commercial P-glucan known for its ~mmunostimulatory effects in several fish species was tested in turbot ScophthalniusmaximusL.. both as an oral immunostimulant and as an adjuvant for oral vaccination. Some non-specific immune parameters were tested after a 5 wk feeding period with a commercial diet mixed with yeast P-glucan. Furthermore, during the last 5 d of the feeding period, half of the fish were orally vaccinated by mixing the commercial pellets with an anti-vibriosis vaccine (Vibriffa bain ND). The oral administration of P-glucan induced no reduction in mortality after a challenge with a virulent Vibrio anguillarum (strain 408). In contrast, a single oral vaccination resulted in protection against V anguillarum. The use of P-glucan as an adjuvant did not reduce the mortality rate more than did the single vaccination. An increase in white blood cell count was observed after the administration of only P-glucan. The plasma complement activity was not influenced by any of the treatments. In contrast, lysozyme activity was enhanced after administration of the adjuvanted vaccine. An increase in the chemiluminescent response of opsonised zymosan-stimulated head-kidney leucocytes was less obvious and no significant results were recorded.

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“…β-1.3/1.6-glucans are characterized by side-chains attached to the backbone that radiate outward like branches on a tree (Figure 4.6.1.1.1). Aakre et al (1994) Yeast glucan Shrimp immersion 43 days ↑ growth at 0.5; 1 and 2 mg/ml but not at 0.25 mg(ml ↑ phenoloxidase activity ↑ resistance to V. vulnificus Sung et al (1994) Yeast glucan Trout i.p n.i ↑ lysozyme activity Thompson et al (1995) Yeast glucan Salmon Oral and anal (150 mg/ 2 days ↑ acid phosphatase Dalmo et al (1996) Norwegian Scientific Committee Sealey et al (2008) Symbols represent an increase (↑) in the specified response; no change (→). n.i -no information given.…”

Section: Norwegian Scientific Committee For Food Safetymentioning

confidence: 99%

Criteria for Safe Use of Plant Ingredients in Diets for Aquacultured Fish

Hemre

Amlund

Aursand

et al. 2018

EJNFS

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Enhanced antibody response in Atlantic salmon (Salmo salar L.) to Aeromonas salmonicida cell wall antigens using a bacterin containing β-1,3-M-Glucan as adjuvant

Cited by 64 publications

References 0 publications

Protective immunity in rainbow trout Oncorhynchus mykiss following immunization with distinct molecular mass fractions isolated from Flavobacterium psychrophilum

Protective immunity in rainbow trout Oncorhynchus mykiss following immunization with distinct molecular mass fractions isolated from Flavobacterium psychrophilum

Effect of long-term oral administration of beta-glucan as an immunostimulant or an adjuvant on some non-specific parameters of the immune response of turbot Scophthalmus maximus

Criteria for Safe Use of Plant Ingredients in Diets for Aquacultured Fish

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