Enhanced angiogenesis by the hyaluronic acid hydrogels immobilized with a VEGF mimetic peptide in a traumatic brain injury model in rats

Lü, Jiaju; Guan, Fengyi; Cui, Fuzhai; Sun, Xiaodan; Zhao, Lingyun; Wang, Ying; Wang, Xiumei

doi:10.1093/rb/rbz027

Cited by 55 publications

(33 citation statements)

References 59 publications

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“…TGF-β could promote mitosis and osteogenic differentiation of osteoprogenitor cells [ 29 ]. VEGF was a key player that regulated the recruitment, survival and activity of endothelial cells, osteoblasts and osteoclasts [ 30 , 31 ]. Figure 3A−C shows that the composite gels containing different concentrations of PRP and LyPRP were soaked in culture medium to simulate the in vitro release behavior of these factors.…”

Section: Resultsmentioning

confidence: 99%

The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

Chen

Liu

et al. 2020

Regenerative Biomaterials

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Although platelet-rich plasma (PRP) plays a significant role in the orthopedic clinical application, it still faces two major problems, namely, uncontrollable factors release, frequent preparation and extraction processes as well as the inconvenient form of usage. To overcome these shortcomings, freeze-dried PRP (LyPRP) was encapsulated into bioactive Col I hydrogel to induce osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs). And PRP/Col І composite hydrogel was prepared as a control. Compared with Col І hydrogel, the introduction of platelets significantly improved the mechanical properties of hydrogels. Meanwhile, platelets were evenly distributed in the composite hydrogels network. The sustainable release of related factors in the composite hydrogels could last for more than 14 days to maintain its long-term biological activity. Further cell experiments confirmed that PRP and LyPRP could effectively alleviate the contraction of collagen hydrogel in vitro, and promote the adhesion, proliferation and osteogenesis differentiation of rBMSCs. The results of osteogenic gene expression indicated that the 10% LyPRP/Col І composite hydrogel could facilitate the early expression of BMP-2 and late osteogenic associated protein formation with higher expression of alkaline phosphatase and Osteocalcin (OCN). These results might provide new insights for the clinical application of 10% LyPRP/Col І composite hydrogel as practical bone repair injection.

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Section: Resultsmentioning

confidence: 99%

The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

Chen

Liu

et al. 2020

Regenerative Biomaterials

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“…Analysis of the microvascular structures marked by an endothelial marker such as CD-31 can be performed through fluorescence microscopy (Costa-Almeida et al, 2015), epifluorescence microscopy (Weinandy et al, 2014), confocal laser scanning microscopy (Costa-Almeida et al, 2015;Dos Santos et al, 2019;Heller et al, 2020;Heller et al, 2016;Ren et al, 2014;Unterleuthner et al, 2017;Xiao et al, 2015), two-photon laser scanning microscopy (TPLSM) (Kniebs et al, 2020;Kreimendahl et al, 2017;Samal et al, 2015;Weinandy et al, 2014), or scanning electron microscopy (SEM) (Lu et al, 2019;Samal et al, 2015;Shen et al, 2017).…”

Section: Immunolabelingmentioning

confidence: 99%

Vascularization strategies in tissue engineering approaches for soft tissue repair

Masson-Meyers

Tayebi

2021

J Tissue Eng Regen Med

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Insufficient vascularization during tissue repair is often associated with poor clinical outcomes. This is a concern especially when patients have critical-sized injuries, where the size of the defect restricts vascularity, or even in small defects that have to be treated under special conditions, such as after radiation therapy (relevant to tumor resection) that hinders vascularity. In fact, poor vascularization is one of the major obstacles for clinical application of tissue engineering methods in soft tissue repair. As a key issue, lack of graft integration, caused by inadequate vascularization after implantation, can lead to graft failure. Moreover, poor vascularization compromises the viability of cells seeded in deep portions of scaffolds/graft materials, due to hypoxia and insufficient nutrient supply. In this article we aim to review vascularization strategies employed in tissue engineering techniques to repair soft tissues. For this purpose, we start by providing a brief overview of the main events during the physiological wound healing process in soft tissues. Then, we discuss how tissue repair can be achieved through tissue engineering, and considerations with regards to the choice of scaffold materials, culture conditions, and vascularization techniques. Next, we highlight the importance of vascularization, along with strategies and methods of prevascularization of soft tissue equivalents, particularly cellbased prevascularization. Lastly, we present a summary of commonly used in vitro methods during the vascularization of tissue-engineered soft tissue constructs.

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“…The functional motif KLT (KLTWQELYQLKYKGI) is a VEGF mimetic peptide and could combined to VEGF receptors and improve the migration, proliferation, and tubulogenesis of ECs [180]. Lu et al modified hyaluronic acid hydrogels with KLT and demonstrated that the HA-KLT hydrogel could improve the spreading, and proliferation of HUVECs in vitro and promoted angiogenesis in vivo [181]. The glycine-histidine-lysine (GHK) peptide is a fragment of osteonectin, and could promote the secretion of VEGF from MSCs in alginate hydrogels [182].…”

Section: Small Moleculers-based Approaches For Vascular Networkmentioning

confidence: 99%

3D Bioprinting for Vascularized Tissue-Engineered Bone Fabrication

et al. 2020

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Vascularization in bone tissues is essential for the distribution of nutrients and oxygen, as well as the removal of waste products. Fabrication of tissue-engineered bone constructs with functional vascular networks has great potential for biomimicking nature bone tissue in vitro and enhancing bone regeneration in vivo. Over the past decades, many approaches have been applied to fabricate biomimetic vascularized tissue-engineered bone constructs. However, traditional tissue-engineered methods based on seeding cells into scaffolds are unable to control the spatial architecture and the encapsulated cell distribution precisely, which posed a significant challenge in constructing complex vascularized bone tissues with precise biomimetic properties. In recent years, as a pioneering technology, three-dimensional (3D) bioprinting technology has been applied to fabricate multiscale, biomimetic, multi-cellular tissues with a highly complex tissue microenvironment through layer-by-layer printing. This review discussed the application of 3D bioprinting technology in the vascularized tissue-engineered bone fabrication, where the current status and unique challenges were critically reviewed. Furthermore, the mechanisms of vascular formation, the process of 3D bioprinting, and the current development of bioink properties were also discussed.

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Enhanced angiogenesis by the hyaluronic acid hydrogels immobilized with a VEGF mimetic peptide in a traumatic brain injury model in rats

Cited by 55 publications

References 59 publications

The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

Vascularization strategies in tissue engineering approaches for soft tissue repair

3D Bioprinting for Vascularized Tissue-Engineered Bone Fabrication

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