Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses

Zhang, Wanjun; Dewey, Ralph E.; Boss, Wendy F.; Phillippy, Brian Q.; Qu, Rongda

doi:10.1007/s11103-012-9997-8

Cited by 62 publications

(56 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is particularly true for the labs with less experience on switchgrass tissue culture. In addition, the transformation protocol we developed (Zhang et al 2013) could have also enhanced Agrobacterium-mediated transformation of switchgrass. The approach we used enhanced lowland switchgrass transformation, which makes it possible for upland cultivar transformation, and could find applications in similar cases in other grass species.…”

Section: Discussionmentioning

confidence: 99%

“…The transformation procedure was carried out in accordance to Zhang et al (2013). A cold treatment was given to calluses (4 months old) in a maltose solution on ice for 20 min prior to Agrobacterium infection.…”

Section: Agrobacterium Strain and Transformationmentioning

confidence: 99%

“…After cocultivation, calluses (about 3 mm in diameter) were cultured on MP medium containing 200 mg l -1 timentin for 7 days for callus recovery and suppression of Agrobacterium growth. MS salts and vitamins, 30 g l -1 maltose, 5.0 mg l -1 2,4-D, 1.0 mg l -1 6-BA, 4.0 g l -1 phytagel MS7 MS basic salt and vitamins, 30 g l -1 Maltose, 7.0 mg l -1 2,4-D, 4.0 g l -1 phytagel NB7 N6 basic salt, 1 9 vitamin B stock A , 30 g l -1 Maltose, 500 mg l -1 CH, 500 mg l -1 L-proline, 7.0 mg l -1 2,4-D, 4.0 g l -1 phytagel MP MS5:1 medium with 2.0 g l -1 L-proline REG MS basic medium with 0.2 mg l -1 NAA, 0.5 mg l -1 GA, 1.0 mg l -1 6-BA, 30 g l -1 maltose, 4.0 g l -1 phytagel SM1 MP medium with 200 mg l -1 timentin and 50 mg l -1 hyg B SM2 MP medium with 200 mg l -1 timentin and 100 mg l -1 hyg B 1/2TM 1/2 MS based medium with 200 mg l -1 timentin and 50 mg l -1 hyg B A 1 9 vitamin B stock was composed of 9.9 mg l -1 thiamine, 9.5 mg l -1 pyridoxine hydrochloride, 4.5 mg l -1 nicotinic acid (Zhang et al 2013) Plant Cell Rep Selection and regeneration of putative transgenic plants After recovery, calluses were transferred to the first selection medium (SM1) containing 50 mg l -1 hyg B (Roche Diagnostics GmbH, Germany). Two weeks later, calluses with vigorous growth were transferred to the second selection medium (SM2) containing 100 mg l -1 hyg B.…”

Section: Agrobacterium Strain and Transformationmentioning

confidence: 99%

See 2 more Smart Citations

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

et al. 2015

Self Cite

View full text Add to dashboard Cite

Selection of pre-embryogenic callus from a core structure from mature seed-derived callus is the key for high-efficiency plant regeneration and transformation of switchgrass different cultivars. Switchgrass (Panicum virgatum L.) has been identified as a dedicated biofuel crop. For its trait improvement through biotechnological approaches, we have developed a highly efficient plant regeneration and genetic transformation protocol for both lowland and upland cultivars. We identified and separated a pre-embryogenic "core" structure from the seed-derived callus, which often leads to development of highly regenerative type II calluses. From the type II callus, plant regeneration rate of lowland cultivars Alamo and Performer reaches 95%, and upland cultivars Blackwell and Dacotah, 50 and 76%, respectively. The type II callus was also amenable for Agrobacterium-mediated transformation. Transformation efficiency of 72.8% was achieved for lowland cultivar Alamo, and 8.0% for upland cultivar Dacotah. PCR, Southern blot and GUS staining assays were performed to verify the transgenic events. High regenerative callus lines could be established in 3 months, and transgenic plants could be obtained in 2 months after Agrobacterium infection. To our knowledge, this is the first report on successful plant regeneration and recovery of transgenic plants from upland switchgrass cultivars by Agrobacterium-mediated transformation. The method presented here could be helpful in breaking through the bottleneck of regeneration and transformation of lowland and upland switchgrass cultivars and probably other recalcitrant grass crops.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Agrobacterium Strain and Transformationmentioning

confidence: 99%

Section: Agrobacterium Strain and Transformationmentioning

confidence: 99%

See 1 more Smart Citation

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Across these four experiments, the average transformation efficiency was 1.3 %. The current protocol gave a low efficiency compared to that of other published protocols of Lolium species such as 4.8 % for L. temulentum (Ge et al 2007) and about 50 % for L. perenne (Zhang et al 2012). The necrosis of calli of this genotype due to the sensitivity to hygromycin may be the major cause.…”

Section: Recovery Of Transgenic Plantsmentioning

confidence: 70%

“…Since the first transgenic Lolium perenne (perennial ryegrass) plants were generated by Spangenberg et al (1995), efforts in Lolium transformation have resulted in successful transformation of L. multiflorum, L. perenne and L. temulentum by biolistic delivery (Ye et al 1997;Dalton et al 1999) and Agrobacterium tumefaciens-mediated methods (Bettany et al 2003;Bajaj et al 2006;Cao et al 2006;Altpeter 2006;Sato and Takamizo 2006;Ge et al 2007;Zhang et al 2012). This has resulted in progress in the development of traits such as improved digestibility (Tu et al 2010), salt tolerance (Wu et al 2005) and cold tolerance (Hisano et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

Ran

Patron

et al. 2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Lolium rigidum Gaud. is an annual grass grown for forage but also an economically damaging crop weed. A single genotype somatic embryogenic callus line, VLR1-60, was identified from a herbicide susceptible L. rigidum population, VLR1, and proved to be amenable to Agrobacterium tumefaciens-mediated transformation. Somatic embryogenic calli were continuously induced from the meristematic region of VLR1-60 plants multiplied in vitro and the basic tolerance level of VLR1-60 to hygromycin B was determined. A hygromycin phosphotransferase gene was used as a selectable marker for hygromycin B selection. Somatic embryogenic calli derived from in vitro grown vegetative tillers were co-cultivated with the A. tumefaciens strain EHA105 harbouring binary vector carrying reporter genes and selectable marker in the presence of acetosyringone for 3 days. Inoculated calli were recovered on callus proliferation medium containing Timentin TM but lacking hygromycin and were then subcultured onto media with hygromycin concentrations increased progressively through time for selection of transformed plant cells. Putative transgenic plants were recovered and integration of transgenes was confirmed by Southern hybridization analysis and by detection of DsRed or GUS activity in transgenic plants. The frequency of plant transformation was 1.3 %. The ability to transform L. rigidum will provide opportunities for functional characterization of genes to improve forage quality and increase our understanding of the evolution of herbicide resistance and of the basic genetics underlying traits that make L. rigidum a damaging crop weed.

show abstract

Metabolic Engineering of Higher Plants and Algae for Isoprenoid Production

Kempinski

Jiang

Bell

et al. 2015

Biotechnology of Isoprenoids

View full text Add to dashboard Cite

Isoprenoids are a class of compounds derived from the five carbon precursors, dimethylallyl diphosphate, and isopentenyl diphosphate. These molecules present incredible natural chemical diversity, which can be valuable for humans in many aspects such as cosmetics, agriculture, and medicine. However, many terpenoids are only produced in small quantities by their natural hosts and can be difficult to generate synthetically. Therefore, much interest and effort has been directed toward capturing the genetic blueprint for their biochemistry and engineering it into alternative hosts such as plants and algae. These autotrophic organisms are attractive when compared to traditional microbial platforms because of their ability to utilize atmospheric CO2 as a carbon substrate instead of supplied carbon sources like glucose. This chapter will summarize important techniques and strategies for engineering the accumulation of isoprenoid metabolites into higher plants and algae by choosing the correct host, avoiding endogenous regulatory mechanisms, and optimizing potential flux into the target compound. Future endeavors will build on these efforts by fine-tuning product accumulation levels via the vast amount of available "-omic" data and devising metabolic engineering schemes that integrate this into a whole-organism approach. With the development of high-throughput transformation protocols and synthetic biology molecular tools, we have only begun to harness the power and utility of plant and algae metabolic engineering.

show abstract

Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses

Cited by 62 publications

References 64 publications

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars

Agrobacterium-mediated transformation of Lolium rigidum Gaud.

Metabolic Engineering of Higher Plants and Algae for Isoprenoid Production

Contact Info

Product

Resources

About