Enhanced 3D spatial resolution in quantitative phase microscopy using spatially incoherent illumination

Bon, Pierre; Aknoun, Shérazade; Monneret, Serge; Wattellier, Benoît

doi:10.1364/oe.22.008654

Cited by 35 publications

(27 citation statements)

References 40 publications

(75 reference statements)

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“…In particular, one interesting application for this interferometric imaging is microscopy, in which synthetic-aperture generation permits the extraction of medium- and high-resolution images using low-NA microscope lenses 57 . In contrast to the proposed illumination scheme using coherent light sources, incoherent illumination of semi-transparent samples has been shown to allow quantitative phase retrieval using quadriwave lateral shearing interferometry 58 . In combination with a number of polarization sensitive detection, the DAM could possibly be extended for multiplexing ellipsometric measurements.…”

Section: Discussionmentioning

confidence: 99%

Super-resolution microscopy with very large working distance by means of distributed aperture illumination

Birk

Hase

Cremer

2017

Sci Rep

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The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer range. Based on numerical simulations of the intensity field distribution, we present an illumination concept for a super-resolution microscope which allows a three dimensional (3D) optical resolution around 150 nm for working distances up to the centimeter regime. In principle, the system allows great flexibility, because the illumination concept can be used to approximate the point-spread-function of conventional microscope optics, with the additional benefit of a customizable pupil function. Compared with the Abbe-limit using an objective lens with such a large working distance, a volume resolution enhancement potential in the order of 104 is estimated.

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Section: Discussionmentioning

confidence: 99%

Super-resolution microscopy with very large working distance by means of distributed aperture illumination

Birk

Hase

Cremer

2017

Sci Rep

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“…Conventionally, 3D QPI is realized via either tomographic [12] or deconvolution methods [13][14][15][16]. In tomography, the object is illuminated over a range of incident angles via either object rotation, in which the sample itself is rotated relative to the imaging system (usually along a principal Cartesian axis), or beam rotation, in which the angle of incidence of the illuminating beam is changed relative to the object and the optical axis of the imaging system [17].…”

Section: Introductionmentioning

confidence: 99%

“…To address these issues, 3D QPI solutions involving the deconvolution of 3D images have been proposed [13][14][15][16]. In such methods, a 3D image is constructed by collecting a through-focal series, after which RI is recovered via 3D deconvolution based on a linearized model.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional quantitative phase imaging via tomographic deconvolution phase microscopy

Jenkins¹,

Gaylord²

2015

Appl. Opt.

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The field of three-dimensional quantitative phase imaging (3D QPI) is expanding rapidly with applications in biological, medical, and industrial research, development, diagnostics, and metrology. Much of this research has centered on developing optical diffraction tomography (ODT) for biomedical applications. In addition to technical difficulties associated with coherent noise, ODT is not congruous with optical microscopy utilizing partially coherent light, which is used in most biomedical laboratories. Thus, ODT solutions have, for the most part, been limited to customized optomechanical systems which would be relatively expensive to implement on a wide scale. In the present work, a new phase reconstruction method, called tomographic deconvolution phase microscopy (TDPM), is described which makes use of commercial microscopy hardware in realizing 3D QPI. TDPM is analogous to methods used in deconvolution microscopy which improve spatial resolution and 3D-localization accuracy of fluorescence micrographs by combining multiple through-focal scans which are deconvolved by the system point spread function. TDPM is based on the 3D weak object transfer function theory which is shown here to be capable of imaging "nonweak" phase objects with large phase excursions. TDPM requires no phase unwrapping and recovers the entire object spectrum via object rotation, mitigating the need to fill in the "missing cone" of spatial frequencies algorithmically as in limited-angle ODT. In the present work, TDPM is demonstrated using optical fibers, including single-mode, polarization-maintaining, and photonic-crystal fibers as well as an azimuthally varying CO2-laser-induced long-period fiber grating period as test phase objects.

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“…Thus, many attempts for capturing digital holograms with low-coherent light sources (LCLS) [8][9][10][11][12][13][14][15][16][17] or by using laser light with reduced coherence, for example, generated by a rotating diffusor [18] were reported. Other approaches have been described in which a reduction of speckle noise is achieved by recording multiple off-axis holograms at different polarizations of object and reference wave in combination with subsequently averaging of the reconstructed amplitude distributions [19].…”

Section: Introductionmentioning

confidence: 99%

Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus tunable lens

Schubert

Vollmer

Ketelhut³

et al. 2014

Biomed. Opt. Express

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Self-interference digital holographic microscopy (DHM) has been found particular suitable for simplified quantitative phase imaging of living cells. However, a main drawback of the self-interference DHM principle are scattering patterns that are induced by the coherent nature of the laser light which affect the resolution for detection of optical path length changes. We present a simple and efficient technique for the reduction of coherent disturbances in quantitative phase images. Therefore, amplitude and phase of the sample illumination are modulated by an electrically focus tunable lens. The proposed method is in particular convenient with the selfinterference DHM concept. Results from the characterization of the method show that a reduction of coherence induced disturbances up to 70 percent can be achieved. Finally, the performance for enhanced quantitative imaging of living cells is demonstrated.

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Enhanced 3D spatial resolution in quantitative phase microscopy using spatially incoherent illumination

Cited by 35 publications

References 40 publications

Super-resolution microscopy with very large working distance by means of distributed aperture illumination

Super-resolution microscopy with very large working distance by means of distributed aperture illumination

Three-dimensional quantitative phase imaging via tomographic deconvolution phase microscopy

Enhanced quantitative phase imaging in self-interference digital holographic microscopy using an electrically focus tunable lens

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