Enhance hydrolytic enzymes production by Aspergillus awamori on supplemented grape pomace

Díaz, Ana Belén; Ory, Ignacio de; Caro, Ildefonso; Blandino, Ana

doi:10.1016/j.fbp.2010.12.003

Cited by 60 publications

(35 citation statements)

References 21 publications

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“…Researchers found that the supplementation of different nitrogen sources, such as NH 4 H 2 PO 4 , urea and yeast extract, showed positive effect on the fermentation performance in the bioconversion processes (Diaz et al, 2012;Hu et al, 2011). Scientists (Botella et al, 2005;Hu et al, 2011) reported that supplementation of nitrogen sources and minerals could be beneficial for the fungal growth in SSF, which agreed with the findings in this study.…”

Section: Nutrient Supplementation For Protein Enrichmentsupporting

confidence: 82%

“…Scientists (Botella et al, 2005;Hu et al, 2011) reported that supplementation of nitrogen sources and minerals could be beneficial for the fungal growth in SSF, which agreed with the findings in this study. Diaz et al (2012) stated that the addition of a nitrogen source and minerals in SSF of grape marc can enhance the enzyme production. Our experimental data revealed that wine lees can be an excellent nutrient source for protein enrichment.…”

Section: Nutrient Supplementation For Protein Enrichmentmentioning

confidence: 99%

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A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

Jin

Zepf

Zhang

et al. 2016

Process Safety and Environmental Protection

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Digestibility Fungi Protein enrichmentWinery biomass waste Solid state fermentation a b s t r a c t Grape marc and lees are main waste biomass in wine industry, which contain substantial amount of carbohydrates and nutrients, but currently deliver considerable carbon emission through landfill in Australia. This study was aimed to develop a biotechnological systematic approach to select suitable fungi for bioconversion of the winery biomass wastes into animal feed. The biotech-systematic approach was developed through assessment of nutrient/carbon source accessibility of the fungi, understanding of their metabolic reactions in solid state fermentation, and nutritive value of the fungi-fermented grape marc. From 13Aspergillus, Rhizopus, and Trichoderma fungal species, A. oryzae DAR 3699, A. oryzae RIB40 and T. reesei RUT C30 were determined as the best fungi due to their promising biochemical capabilities to enhance protein contents and in vitro digestibility of grape marc in mono-and co-cultivations. The mono-fungous-cultured SSF process used these selected fungi is able to convert grape marc to nutrient-rich feed by increasing the protein contents from ∼5% to 26% and the digestibility from ∼25% to over 50%. R. oligosporus 2710 with R. oryzae 6201 or T.viride 15719 showed promising protein enrichment to 18-23% and digestibility improvement by ∼50% in their co-cultivations. This preliminary bioengineering strategy will be useful for selecting microorganisms for the bioconversion of organic wastes to high valuable products.This "green cycle" bioprocess will be useful to promote the old-fashioned waste treatment technologies for the cleaner production in food processing industries.

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Section: Nutrient Supplementation For Protein Enrichmentsupporting

confidence: 82%

Section: Nutrient Supplementation For Protein Enrichmentmentioning

confidence: 99%

A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

Jin

Zepf

Zhang

et al. 2016

Process Safety and Environmental Protection

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“…Increasing the Ms quantity stimulated fungal growth and PG production, this result may be due to its composition (pectic substance, protein, mineral salts, vitamins, etc.). High levels of exo-PG, xylanase or CMC-ase activities can be obtained using a mixture of grape pomace and orange peels (1:1) in SSF (Díaz et al, 2012).…”

Section: Effect Of Different Ob and Ms Ratiomentioning

confidence: 99%

Utilization of orange bagasse and molokhia stalk for production of pectinase enzyme

Ahmed

Mostafa

2013

Braz. J. Chem. Eng.

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-Studies were conducted on the production and extraction of exo-polygalacturonase (PG) in solidstate fermentation (SSF) using orange bagasse (Ob) and molokhia stalks (Ms) as a new solid support by Penicillium pinophlilum Hedg 3503 NRRL. The parameters affecting PG production under SSF were optimized. The maximum PG activity of ~ 3270 U/ g dry solid substrate was obtained from P. pinophlilum Hedg 3503 NRRL grown for 7 days on Ob and Ms in the ratio 1:3 (w/w), moistened with distilled water at 68.2% initial moisture content. Highest enzyme titers occurred in SSF without added nutrients, indicating nutrient sufficiency of the Ob and MS mixture to sustain growth and a high level of pectic substances which induced PG production. The extraction of PG from the fermented biomass was optimized. Among the various solvents tested, the maximum level of enzyme activity was achieved when acetate buffer (0.05 M; pH 5.0) was used. The optimum volume of buffer was 50 mL, an extraction time of 60 min was sufficient to extract most enzyme, which yielded 3269.6 U/ g dry solid substrate of enzyme activity. Repeated washes under the optimum conditions showed that most of the enzyme (about 98%) was recovered in three repeated extractions. Moreover, 68.9% of total activity was achieved in the first wash.

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“…The required amount of spore suspension and the exact amount of nutrient solution at pH 5.0, containing (g/L) 2.4 urea, 9.8 g (NH4)2SO4, 5.0 KH2PO4, 0.001 FeSO4·7H2O, 0.0008 ZnSO4·7H2O, 0.004 MgSO4·7H2O and 0.001 CuSO4·5H2O, to obtain an initial moisture content of 70 % were poured into disposable Petri dishes (9 cm diameter). Moreover, this solution supplemented with 11.5 g/L of pectin to induce the production of pectinases was used for grape pomace (Díaz et al 2012). Every experiment was developed in duplicate.…”

Section: Solid State Fermentationmentioning

confidence: 99%

Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues

Díaz

Blandino

Webb

et al. 2016

Appl Microbiol Biotechnol

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A simple kinetic model, with only three fitting parameters, for several enzymes production in Petri dishes by solid state fermentation is proposed in this paper, which may be a valuable tool for simulation of this type of processes. Basically, the model is able to predict temporal fungal enzymes production by solid state fermentation on complex substrates, maximum enzyme activity expected and time at which these maximums are reached. In this work several fermentations in solid state were performed in Petri dishes, using four filamentous fungi grown on different agro-industrial residues, measuring xylanase, exopoligalacturonase, cellulose and laccase activities over time. Regression coefficients after fitting experimental data to the proposed model turned out to be quite high in all cases. In fact, these results are very interesting considering on the one hand, the simplicity of the model, and on the other hand, that enzyme activities correspond to different enzymes, produced by different fungi on different substrates.

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Enhance hydrolytic enzymes production by Aspergillus awamori on supplemented grape pomace

Cited by 60 publications

References 21 publications

A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

Utilization of orange bagasse and molokhia stalk for production of pectinase enzyme

Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues

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