A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

Abstract

Digestibility Fungi Protein enrichmentWinery biomass waste Solid state fermentation a b s t r a c t Grape marc and lees are main waste biomass in wine industry, which contain substantial amount of carbohydrates and nutrients, but currently deliver considerable carbon emission through landfill in Australia. This study was aimed to develop a biotechnological systematic approach to select suitable fungi for bioconversion of the winery biomass wastes into animal feed. The biotech-systematic approach was developed through assessment of nutrient/carbon source accessibility of the fungi, understanding of their metabolic reactions in solid state fermentation, and nutritive value of the fungi-fermented grape marc. From 13Aspergillus, Rhizopus, and Trichoderma fungal species, A. oryzae DAR 3699, A. oryzae RIB40 and T. reesei RUT C30 were determined as the best fungi due to their promising biochemical capabilities to enhance protein contents and in vitro digestibility of grape marc in mono-and co-cultivations. The mono-fungous-cultured SSF process used these selected fungi is able to convert grape marc to nutrient-rich feed by increasing the protein contents from ∼5% to 26% and the digestibility from ∼25% to over 50%. R. oligosporus 2710 with R. oryzae 6201 or T.viride 15719 showed promising protein enrichment to 18-23% and digestibility improvement by ∼50% in their co-cultivations. This preliminary bioengineering strategy will be useful for selecting microorganisms for the bioconversion of organic wastes to high valuable products.This "green cycle" bioprocess will be useful to promote the old-fashioned waste treatment technologies for the cleaner production in food processing industries.

Grape Winery Waste as Feedstock for Bioconversions: Applying the Biorefinery Concept

“…Trials have been made by treating grape marc with fungi (Aspergillus, Rhizopus and Trichoderma ssp.) to enhance protein content in order to provide a nutritious animal feed (protein contents increase between 5 and 26 % and digestibility increased from 25 to 50 %) [90].…”

Mediterranean agro‐industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid‐state fermentation

“…Bioprocessing of wastes by SSF is a low‐cost technique to obtain nutritive animal feed . Several workers have demonstrated the protein enhancement of agro‐food wastes by fungi of around 5–26% . On the other hand, lignocellulosic wastes are considered as being of low digestibility, but SSF by fungi can improve the digestibility by enzymes production, since these can break the bonds of hemicellulose and cellulose with the lignin.…”

“…7 Several workers have demonstrated the protein enhancement of agro-food wastes by fungi of around 5-26%. [8][9][10] On the other hand, lignocellulosic wastes are considered as being of low digestibility, 11 but SSF by fungi can improve the digestibility by enzymes production, since these can break the bonds of hemicellulose and cellulose with the lignin. In addition, the SSF of lignocellulosic materials can increase the degradability of dietary protein in the rumen.…”

“…Fungi comply with several of these aspects as they have high protein content, are safe for human and animals and have the ability to grow in agro-industrial wastes. 10 Aspergillus niger is a filamentous fungus considered as generally regarded as safe by the US Food and Drug Administration. Aspergillus ibericus and Aspergillus uvarum are species isolates from grapes unable produce mycotoxins.…”

Cascas Do Processamento De Palmito Para Uso Na Alimentação Humana: Uma Abordagem Socioambiental

“…A degradação dos resíduos agroindustriais em relação aos aspectos biológicos, pode ser realizada por diferentes organismos, porém os fungos são os principais agentes degradadores de matéria orgânica. Os fungos são frequentemente utilizados por serem capazes de penetrar nos substratos sólidos, promovendo a bioconversão de resíduos orgânicos agroindustriais (JIN et al, 2016).…”