Engraftment of a clonal bone marrow stromal cell line in vivo stimulates hematopoietic recovery from total body irradiation.

Anklesaria, Pervin; Kase, Kenneth R.; Glowacki, Julie; Holland, Christie A.; Sakakeeny, Mary Ann; Wright, James R.; Fitzgerald, Thomas J.; Lee, Chi-Yu; Greenberger, Joel S.

doi:10.1073/pnas.84.21.7681

Cited by 140 publications

(91 citation statements)

References 22 publications

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“…28 Engraftment of a clonal murine bone marrow stromal cell line in irradiated mice has been described, which stimulated hematopoietic recovery after total body irradiation. 29 Donor stromal cells were detected only in the bone marrow of recipients but other studies indicate an ability for bone marrow stromal cells to engraft other tissues as well. Expanded cultured adherent cells from the bone marrow of transgenic mice expressing a minigene for human collagen I were injected into irradiated mice and later detected in several tissues: marrow, spleen, bone, lung and cartilage.…”

Section: Figurementioning

confidence: 96%

Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice

et al. 1998

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The non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mouse is a convenient host for human hematopoietic tissues and cells. Human fetal bone fragments engrafted subcutaneously in NOD-SCID mice sustain human hematopoiesis for several months. MS5 murine bone marrow stromal cells were transfected by electroporation with a plasmid containing the human interleukin-3 gene. As expected, stably transfected hu-IL3-MS5 cells supported human hematopoiesis in vitro more efficiently than MS5 cells. hu-IL3-MS5 cells were then injected intravenously into hu-NOD-SCID mice to test their ability to home to the mouse and/or human bone marrow, and to evaluate the role of hu-IL3 secretion on human hematopoiesis in vivo. hu-IL3 was detected in the mouse serum for up to an observation time of 8 weeks. hu-IL3-MS5 cells engrafted the bone marrow, spleen, liver and lungs of the mice but also the human bone graft. The presence of hu-IL3-MS5 cells in the human bone significantly stimulated local human hematopoiesis. This setting could be used to model the bone marrow homing of intravenously injected stromal cells or stromal cell precursors. The same experimental principle could also be applied in a therapeutic perspective to malignant human bone marrow hematopoiesis.

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Section: Figurementioning

confidence: 96%

Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice

et al. 1998

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“…7,29,30 A number of preclinical animal models of stromal cell transplantation have been established to support hematopoietic engraftment, including using human fetal lung-derived MSCs. [31][32][33] Both autologous and allogeneic human marrow stromal cells were shown to improve the quantity and duration of human hematopoietic engraftment in a preimmune sheep model. 34 Angelopoulou et al 35 recently reported that cotransplantation of human MSCs enhanced myelopoiesis and megakaryopoiesis in NOD-SCID mice transplanted with the limiting doses of peripheral blood CD34 þ cells.…”

Section: Discussionmentioning

confidence: 99%

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

Zhao

Liao

Jiang

et al. 2005

Bone Marrow Transplant

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Summary:Human mesenchymal stem cells (hMSCs) are excellent candidates for ex vivo gene transfer and cell therapy in various systems. However, hMSCs are mortal somatic cells, and thus invariably enter an irreversible growth arrest after a finite number of cell divisions in culture. It has been proposed that this is due to telomere shortening. In this study, pGRN145 plasmid containing human telomerase reverse transcriptase (hTRT) was introduced into fetal dermis-derived hMSCs. Single-cell clones positive for telomerase activity and hTRT mRNA were selected and expanded. Single-cell-derived hTRT þ cells could be expanded rapidly in vitro and passaged up to 70 doublings without showing senescence. FACScan flow cytometer showed that hTRT þ cells were positive for CD29, CD44, CD105, and CD166, while CD31, CD45, CD34, vWF, and HLA-DR were negative. Under suitable conditions, hTRT þ cells have the ability of multiple lineage differentiation, including bone, fat, and nerve. Furthermore, transplantation of hTRT þ cells could protect NOD/SCID mice from lethal irradiation. Thus, these cells may be an ideal cell source for promoting hematopoietic recovery after radiotherapy. Bone Marrow Transplantation (2005) 36, 355-365.

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“…Our data, and the studies of others, indicate that stromal cell infusion can influence host hematopoiesis 14,15,33,34 and suggest that this approach has possible application in cell and/or gene therapy. 35,36 The use of stromal cells has the added dimension of extensive transdifferentiation potential to osteoblasts, chondroblasts, 37,38 fibroblasts, 39 muscle, 40 astrocytes, 41 and neurons.…”

Section: Discussionmentioning

confidence: 86%

“…14 Anklesaria et al 15 showed that infusion of a transformed stromal cell line into irradiated and splenectomized Sl/Sl d mice could partially correct their anemia. The aim of our study is to extend this observation by transplanting normal stromal cells, derived from long-term bone marrow cultures, into unconditioned Sl/Sl d recipients as a model for human cell therapy.…”

mentioning

confidence: 99%

W/Wv marrow stromal cells engraft and enhance early erythropoietic progenitors in unconditioned Sl/Sld murine recipients

et al. 2002

Bone Marrow Transplant

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Engraftment of a clonal bone marrow stromal cell line in vivo stimulates hematopoietic recovery from total body irradiation.

Cited by 140 publications

References 22 publications

Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice

Transplantation of stromal cells transduced with the human IL3 gene to stimulate hematopoiesis in human fetal bone grafts in non-obese, diabetic-severe combined immunodeficiency mice

Establishment and properties of fetal dermis-derived mesenchymal stem cell lines: plasticity in vitro and hematopoietic protection in vivo

W/Wv marrow stromal cells engraft and enhance early erythropoietic progenitors in unconditioned Sl/Sld murine recipients

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