W/Wv marrow stromal cells engraft and enhance early erythropoietic progenitors in unconditioned Sl/Sld murine recipients

Sj, Bubnic; Xh, Wang; Br, Clark; Keating, Armand

doi:10.1038/sj.bmt.1703761

Cited by 7 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Bubnic et al demonstrated that W/Wv mouse - derived marrow stromal cells engrafted into the marrow compartment and enhanced early erythropoiesis in unconditioned Sl/Sld murine recipients. Engraftment of donor stromal cells reached levels of up to 1.0% of total marrow cells 4 months post transplantation 52 . This report was interesting because transplanted MSC do not usually replace the endogenous microenvironment, even after conditioning.…”

Section: Systemic Cytokine Production From Genetically Engineered mentioning

confidence: 98%

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Meyerrose

Olson

Pontow

et al. 2010

Advanced Drug Delivery Reviews

157

117

View full text Add to dashboard Cite

Section: Systemic Cytokine Production From Genetically Engineered mentioning

confidence: 98%

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Meyerrose

Olson

Pontow

et al. 2010

Advanced Drug Delivery Reviews

157

117

View full text Add to dashboard Cite

“…These MSCs were cultured in long-term marrow culture medium according to our previously described method [26]. Briefly, 4 -5 ϫ 10 7 MSCs per mouse were collected and cultured using a long-term marrow culture medium consisting of 12.5% fetal bovine serum, 12.5% horse serum, 1% antibiotic-antimycotic solution, 1% glutamine, 1% vitamin solution, 0.8% essential amino acid, 0.4% nonessential amino acid, 1% sodium pyruvate, 1% sodium bicarbonate solution, 0.036% hydrocortisone (Sigma-Aldrich, St. Louis, http://www.sigmaaldrich.…”

Section: Bone Marrow-derived Mesenchymal Stromal Cellsmentioning

confidence: 99%

Bone Marrow-Derived Mesenchymal Stromal Cells Express Cardiac-Specific Markers, Retain the Stromal Phenotype, and Do Not Become Functional Cardiomyocytes In Vitro

et al. 2008

View full text Add to dashboard Cite

Although cell fusion between rCMs and MSCs was detectable, the very low frequency (0.7%) could not account for the phenotype of the GFP ؉ MSCs. In conclusion, we have identified an MSC population displaying plasticity toward the cardiomyocyte lineage while retaining mesenchymal stromal cell properties, including a nonexcitable electrophysiological phenotype. The demonstration of an MSC population coexpressing cardiac and stromal cell markers may explain conflicting results in the literature and indicates the need to better understand the effects of MSCs on myocardial injury. STEM

show abstract

“…Both mKitL and sKitL may bind c-kit; however, the signaling mechanism differs. For instance, the Steel-Dickie mouse, which is deficient in mKitL, has a defect in the hematopoietic supportive microenvironment within the bone marrow despite physiological levels of sKitL and is unable to accept bone marrow transplantation (29). Cleavage of mKitL to sKitL may occur by proteolytic enzymes (30).…”

mentioning

confidence: 99%

Activation of c‐kit is necessary for mobilization of reparative bone marrow progenitor cells in response to cardiac injury

et al. 2007

View full text Add to dashboard Cite

Cardiovascular disease is the number-one cause of mortality in the developed world. The aim of this study is to define the mechanisms by which bone marrow progenitor cells are mobilized in response to cardiac ischemic injury. We used a closed-chest model of murine cardiac infarction/reperfusion, which segregated the surgical thoracotomy from the induction of cardiac infarction, so that we could study isolated fluctuations in cytokines without the confounding impact of surgery. We show here that bone marrow activation of the c-kit tyrosine kinase receptor in response to released soluble KitL is necessary for bone marrow progenitor cell mobilization after ischemic cardiac injury. We also show that release of KitL and c-kit activation require the activity of matrix metalloproteinase-9 within the bone marrow compartment. Finally, we demonstrate that mice with c-kit dysfunction develop cardiac failure after myocardial infarction and that bone marrow transplantation rescues the failing cardiac phenotype. In light of the ongoing trials of progenitor cell therapy for heart disease, our study outlines the endogenous repair mechanisms that are invoked after cardiac injury. Amplification of this pathway may aid in restoration of cardiac function after myocardial infarction.

show abstract

W/Wv marrow stromal cells engraft and enhance early erythropoietic progenitors in unconditioned Sl/Sld murine recipients

Abstract: Our data suggest that in this murine model, splenic erythropoiesis may influence stromal cell therapy, and that higher levels of marrow engraftment may be necessary to obtain a clinically significant effect.

Cited by 7 publications

References 38 publications

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Bone Marrow-Derived Mesenchymal Stromal Cells Express Cardiac-Specific Markers, Retain the Stromal Phenotype, and Do Not Become Functional Cardiomyocytes In Vitro

Activation of c‐kit is necessary for mobilization of reparative bone marrow progenitor cells in response to cardiac injury

Contact Info

Product

Resources

About