Engineering Zinc Finger Protein Transcription Factors: The Therapeutic Relevance of Switching Endogenous Gene Expression On or Off at Command

Gommans, Willemijn M.; Haisma, Hidde J.; Rots, Marianne G.

doi:10.1016/j.jmb.2005.06.082

Cited by 57 publications

(36 citation statements)

References 67 publications

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“…For example, in vitro DBPs of TFs can be used to guide the design and selection of artificial TFs (Gommans et al 2005, Klug 2005, small molecules of TF mimics (Kwon et al 2004, Xiao et al 2007, Block et al 2009, Rodriguez-Martinez et al 2010, Kushal et al 2011, and TF decoys (Penolazzi et al 2007, Tomita et al 2007, which can be developed as drugs for transcription therapy. The in vitro DBPs can also accelerate the creation of precisiontailored DNA therapeutics (Carlson et al 2010).…”

Section: Dsdna Microarraymentioning

confidence: 99%

In vitro DNA-binding profile of transcription factors: methods and new insights

Wang¹,

Lü²,

Gu³

et al. 2011

Journal of Endocrinology

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The DNA-binding specificity of transcription factors (TFs) has broad impacts on cell physiology, cell development and in evolution. However, the DNA-binding specificity of most known TFs still remains unknown. The specificity of a TF protein is determined by its relative affinity to all possible binding sites. In recent years, the development of several in vitro techniques permits high-throughput determination of relative binding affinity of a TF to all possible k bp-long DNA sequences, thus greatly promoting the characterization of DNA-binding specificity of many known TFs. All DNA sequences that can be bound by a TF with various binding affinities form their DNA-binding profile (DBP). The DBP is important to generate an accurate DNA-binding model, identify all DNA-binding sites and target genes of TFs in the whole genome, and build transcription regulatory network. This study reviewed these techniques, especially two master techniques: double-stranded DNA microarray and systematic evolution of ligands by exponential enrichment in combination with parallel DNA sequencing techniques (SELEX-seq).

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Section: Dsdna Microarraymentioning

confidence: 99%

In vitro DNA-binding profile of transcription factors: methods and new insights

Wang¹,

Lü²,

Gu³

et al. 2011

Journal of Endocrinology

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“…The DNA-binding domains of zinc finger proteins, transcription-activator-like effectors (TALEs), or CRISPR/Cas nuclease can be genetically fused to transcriptional effector domains to function as designed site-specific transcriptional activators or repressors targeted against almost any DNA sequence [13][14][15][16][17][18][19][20] . The ability to design these synthetic transcription factors to target a large sequential diversity of DNA operators should support the construction of complex designed genetic circuits.…”

mentioning

confidence: 99%

A bistable genetic switch based on designable DNA-binding domains

et al. 2014

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“…The application of phage display technology include determination of binding partners of organic (proteins, polysaccharides, or DNAs) (Gommans et al, 2005) or inorganic materials (Hattori et al, 2010;Whaley et al, 2000). The technique is also used to study enzyme evolution in vitro for engineering biocatalysts (Pedersen et al, 1998).…”

Section: Applications Of Phage Display 421 General Applicationsmentioning

confidence: 99%