Engineering Yeast <i>Yarrowia lipolytica</i> for Methanol Assimilation

Wang, Guokun; Olofsson-Dolk, Mattis; Hansson, Frederik Gleerup; Donati, Stefano; Li, Xin; Chang, Hong; Cheng, Jian; Dahlin, Jonathan; Borodina, Irina

doi:10.1021/acssynbio.1c00464

Cited by 28 publications

(40 citation statements)

References 54 publications

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“…In another study, the transplantation of the DHA cycle genes from P. pastoris supported S. cerevisiae growth on methanol ( Dai et al, 2017 ). Also, another non-methylotrophic yeast Yarrowia lipolytica has been recently engineered to assimilate methanol by introducing the DHA and RuMP cycle genes, as well as by using laboratory evolution ( Wang et al, 2021 ).…”

Section: Metabolic Engineering Of Microbes To Use Next-generation Fee...mentioning

confidence: 99%

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Zhang

Ottenheim

Weingarten

et al. 2022

Front. Bioeng. Biotechnol.

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Global shift to sustainability has driven the exploration of alternative feedstocks beyond sugars for biomanufacturing. Recently, C1 (CO2, CO, methane, formate and methanol) and C2 (acetate and ethanol) substrates are drawing great attention due to their natural abundance and low production cost. The advances in metabolic engineering, synthetic biology and industrial process design have greatly enhanced the efficiency that microbes use these next-generation feedstocks. The metabolic pathways to use C1 and C2 feedstocks have been introduced or enhanced into industrial workhorses, such as Escherichia coli and yeasts, by genetic rewiring and laboratory evolution strategies. Furthermore, microbes are engineered to convert these low-cost feedstocks to various high-value products, ranging from food ingredients to chemicals. This review highlights the recent development in metabolic engineering, the challenges in strain engineering and bioprocess design, and the perspectives of microbial utilization of C1 and C2 feedstocks for the biomanufacturing of value-added products.

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Section: Metabolic Engineering Of Microbes To Use Next-generation Fee...mentioning

confidence: 99%

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Zhang

Ottenheim

Weingarten

et al. 2022

Front. Bioeng. Biotechnol.

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“…In addition to employing C 1 -utilizers, constructing synthetic C 1 -utilizing pathways in mature biotechnological platforms is also gaining increasing attention in C 1 -based biomanufacturing areas [ 60 ]. Biotechnologically relevant organisms such as prokaryotic E. coli , eukaryotic Pichia pastoris, and Yarrowia lipolytica have been recently rewired to be capable of growing on formate, methanol or/and CO 2 , shedding light on the sustainable and low-cost synthesis of isobutyraldehyde in these industrial strains [ 4 – 7 , 61 , 62 ]. However, many challenges such as metabolite and cofactor imbalance, enzymes constraints, substrates and intermediates toxicity remain to be addressed to further take advantage of C 1 metabolism for isobutyraldehyde synthesis.…”

Section: In Vivo Framework Converting C 1 Feedstoc...mentioning

confidence: 99%

Toward bioproduction of oxo chemicals from C1 feedstocks using isobutyraldehyde as an example

Guo

Sun

Huo

2022

Biotechnol Biofuels

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Oxo chemicals are valuable chemicals for synthesizing a wide array of industrial and consumer products. However, producing of oxo chemicals is predominately through the chemical process called hydroformylation, which requires petroleum-sourced materials and generates abundant greenhouse gas. Current concerns on global climate change have renewed the interest in reducing greenhouse gas emissions and recycling the plentiful greenhouse gas. A carbon–neutral manner in this regard is producing oxo chemicals biotechnologically using greenhouse gas as C1 feedstocks. Exemplifying isobutyraldehyde, this review demonstrates the significance of using greenhouse gas for oxo chemicals production. We highlight the current state and the potential of isobutyraldehyde synthesis with a special focus on the in vivo and in vitro scheme of C1-based biomanufacturing. Specifically, perspectives and scenarios toward carbon– and nitrogen–neutral isobutyraldehyde production are proposed. In addition, key challenges and promising approaches for enhancing isobutyraldehyde bioproduction are thoroughly discussed. This study will serve as a reference case in exploring the biotechnological potential and advancing oxo chemicals production derived from C1 feedstocks.

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“…A chimeric methanol assimilation pathway was engineered in Y. lipolytica by introducing both the RuMP and the XumP pathways ( Wang et al, 2021 ). In this strain, methanol was oxidised by the Mdh from B. stearothermophilus .…”

Section: And Match Enzymesmentioning

confidence: 99%

From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains

Peiro

Vicente

Jallet

et al. 2022

Front. Bioeng. Biotechnol.

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Engineering microorganisms to grow on alternative feedstocks is crucial not just because of the indisputable biotechnological applications but also to deepen our understanding of microbial metabolism. One-carbon (C1) substrate metabolism has been the focus of extensive research for the prominent role of C1 compounds in establishing a circular bioeconomy. Methanol in particular holds great promise as it can be produced directly from greenhouse gases methane and carbon dioxide using renewable resources. Synthetic methylotrophy, i.e. introducing a non-native methanol utilization pathway into a model host, has therefore been the focus of long-time efforts and is perhaps the pinnacle of metabolic engineering. It entails completely changing a microorganism’s lifestyle, from breaking up multi-carbon nutrients for growth to building C-C bonds from a single-carbon molecule to obtain all metabolites necessary to biomass formation as well as energy. The frontiers of synthetic methylotrophy have been pushed further than ever before and in this review, we outline the advances that paved the way for the more recent accomplishments. These include optimizing the host’s metabolism, “copy and pasting” naturally existing methylotrophic pathways, “mixing and matching” enzymes to build new pathways, and even creating novel enzymatic functions to obtain strains that are able to grow solely on methanol. Finally, new approaches are contemplated to further advance the field and succeed in obtaining a strain that efficiently grows on methanol and allows C1-based production of added-value compounds.

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Engineering Yeast Yarrowia lipolytica for Methanol Assimilation

Cited by 28 publications

References 54 publications

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Toward bioproduction of oxo chemicals from C1 feedstocks using isobutyraldehyde as an example

From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains

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