Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation

Yoshida, Kouki; Sakamoto, Shingo; Kawai, Tetsushi; Kobayashi, Yoshinori; Sato, Kazuhito; Ichinose, Yasunori; Yaoi, Katsuro; Akiyoshi-Endo, Miho; Sato, Hiroko; Takamizo, Tadashi; Ohme‐Takagi, Masaru; Mitsuda, Nobutaka

doi:10.3389/fpls.2013.00383

Cited by 97 publications

(80 citation statements)

References 48 publications

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“…To construct the Pro ESR1 :ESR1-GFP and Pro ESR1 :ESR1-SRDX vectors, genomic fragments containing the 2000-bp promoter sequence and ESR1 coding sequence were amplified by PCR and cloned into the pGFP_NOSG and pSRDX_NOSG vectors, respectively (Yoshida et al, 2013). The resulting Pro ESR1 :ESR1-GFP and Pro ESR1 :ESR1-SRDX fragments were subcloned into the pBCKH vector by Gateway LR Clonase II (Life technologies) for plant transformation (Mitsuda et al, 2006).…”

Section: Plasmid Constructionmentioning

confidence: 99%

WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis

et al. 2016

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Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain largely unknown. We previously identified an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) as a central regulator of wound-induced cellular reprogramming in plants. In this study, we demonstrate that WIND1 promotes callus formation and shoot regeneration by upregulating the expression of the ENHANCER OF SHOOT REGENERATION1 (ESR1) gene, which encodes another AP2/ERF transcription factor in Arabidopsis thaliana. The esr1 mutants are defective in callus formation and shoot regeneration; conversely, its overexpression promotes both of these processes, indicating that ESR1 functions as a critical driver of cellular reprogramming. Our data show that WIND1 directly binds the vascular system-specific and wound-responsive cis-element-like motifs within the ESR1 promoter and activates its expression. The expression of ESR1 is strongly reduced in WIND1-SRDX dominant repressors, and ectopic overexpression of ESR1 bypasses defects in callus formation and shoot regeneration in WIND1-SRDX plants, supporting the notion that ESR1 acts downstream of WIND1. Together, our findings uncover a key molecular pathway that links wound signaling to shoot regeneration in plants.

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Section: Plasmid Constructionmentioning

confidence: 99%

WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis

et al. 2016

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“…Protoplasts were isolated from Arabidopsis rosette leaves grown for 3-4 weeks using the Tape-Arabidopsis Sandwich method (Wu et al 2009) and prepared as previously described (Yoshida et al 2013). Each effector plasmid was co-transfected with a reporter plasmid containing 5×GAL4-TATA-Luciferase (Hiratsu et al 2004) and an internal control plasmid (phRLHSP) (Oshima et al 2013) expressing modified Renilla luciferase.…”

Section: Transient Luciferase Assaymentioning

confidence: 99%

WUSCHEL-RELATED HOMEOBOX 2 is a transcriptional repressor involved in lateral organ formation and separation in <i>Arabidopsis</i>

Chung

Sakamoto

Mitsuda

et al. 2016

Plant Biotechnology

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In this study, we characterized the function of WUSCHEL-RELATED HOMEOBOX 2 (WOX2) using overexpression, CRES-T, and VP16 fusion techniques. Although the function of WOX2 has been described mainly in embryogenesis, it was unclear whether it also plays a role in the post-embryogenic developmental stage. We found that WOX2 has transcriptional repression activity and that either overexpression of WOX2 or expression of its chimeric repressor causes severe growth defects and other morphological phenotypes by impairing plant organ formation and separation. By contrast, VP16-fused WOX2-expressing plants did not display such severe phenotypic defects. In addition, some of them displayed phenotypic defects such as fusion of organs and induction of undifferentiated cells in the boundary regions of organs where GUS staining was clearly observed in the proWOX2:GUS transgenic plants. We suggest that WOX2 is involved in regulation of lateral organ formation and separation during the post-embryogenic development processes.

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“…For example, the regulatory function in SCW formation is shared by a series of NAC TFs from dicots (Kubo et al 2005;Zhong et al 2006Zhong et al , 2010Mitsuda et al 2007;Ohashi-Ito et al 2010;Zhao et al 2010;Ohtani et al 2011) and grasses (Zhong et al 2011;Yoshida et al 2013). Maize ZmMYB31 (Fornalé et al 2010) and switchgrass PvMYB4 (Shen et al 2012) are repressors for lignin biosynthesis as with these Arabidopsis orthologs, AtMYB4.…”

Section: Introductionmentioning

confidence: 98%

The expression of a rice secondary wall-specific cellulose synthase gene, OsCesA7, is directly regulated by a rice transcription factor, OsMYB58/63

Noda

Koshiba

Hattori

et al. 2015

Planta

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A rice MYB transcription factor, OsMYB58/63, was found to directly upregulate the expression of a rice secondary wall-specific cellulose synthase gene, cellulose synthase A7 ( OsCesA7 ); in contrast, the Arabidopsis putative orthologs AtMYB58 and AtMYB63 have been shown to specifically activate lignin biosynthesis. Although indirect evidence has shown that grass plants are similar to but partially different from dicotyledonous ones in transcriptional regulation of lignocellulose biosynthesis, little is known about the differences. This study showed that a rice MYB transcription factor, OsMYB58/63, directly upregulated the expression of a rice secondary wall-specific cellulose synthase gene, cellulose synthase A7 (OsCesA7). Gene co-expression analysis showed that, in rice, OsMYB58/63 and several rice MYB genes were co-expressed with genes encoding lignocellulose biosynthetic enzymes. The expression levels of OsMYB55/61, OsMYB55/61-L, OsMYB58/63, and OsMYB42/85 were commonly found to be high in culm internodes and nodes. All four MYB transcription factors functioned as transcriptional activators in yeast cells. OsMYB58/63 most strongly transactivated the expression of OsCesA7 in rice protoplasts. Moreover, recombinant OsMYB58/63 protein was bound to two distinct cis-regulatory elements, AC-II and SMRE3, in the OsCesA7 promoter. This is in sharp contrast to the role of Arabidopsis orthologs, AtMYB58 and AtMYB63, which had been reported to specifically activate lignin biosynthesis. The promoter analysis revealed that AC elements, which are the binding sites for MYB58 and MYB63, were lacking in cellulose and xylan biosynthetic genes in Arabidopsis, but present in cellulose, xylan, and lignin biosynthetic genes in rice, implying that the difference of transcriptional regulation between rice and Arabidopsis is due to the distinct composition of promoters. Our results provide a new insight into transcriptional regulation in grass lignocellulose biosynthesis.

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Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation

Cited by 97 publications

References 48 publications

WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis

WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis

WUSCHEL-RELATED HOMEOBOX 2 is a transcriptional repressor involved in lateral organ formation and separation in <i>Arabidopsis</i>

The expression of a rice secondary wall-specific cellulose synthase gene, OsCesA7, is directly regulated by a rice transcription factor, OsMYB58/63

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