Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO₂

Abstract

The methylerythritol phosphate pathway in photosynthetic cyanobacteria was engineered to allow highly efficient production of isoprene from CO2.

Photosynthetic fuel for heterologous enzymes: the role of electron carrier proteins

“…Formation of the final electron transfer complex involves sampling of possible interactions by surface diffusion and is stabilized mainly by interactions between small hydrophobic surface patches. Fast dissociation of the complex occurs because resolvation of surface charges is thermodynamically favorable et al 2016), as well as complex multistep pathways like an optimized methylerythritol phosphate pathway for isoprene production (Gao et al 2016) and a de novo engineered 2,3-butanediol biosynthetic pathway (Oliver et al 2013). However, some redox enzymes require dedicated reductases to supply reducing power for catalysis, which may complicate engineering because of a need to adjust both enzyme and reductase activity (Paddon and Keasling 2014).…”

Selective Production of Toluene from Biomass‐Derived Isoprene and Acrolein

“…[10] Another wayt op roduce aromatics from biomass-derived platform compounds was reported by the groups of Frost, [2b] Dauenhauer, [11] Davis, [12] and other researchers. So far,t he highest yield of 35.2 %w as obtained using gas-phase co-feeding of propylenea nd furan over Bio-derived isoprene can be produced by fermentation of glucose and lignocellulose, [15] or through the isoprene-biosynthetic pathway direct from CO 2 , [16] which shows potential for To luene is ab asic chemical that is currently produced from petroleumr esources.I nt his paper,w er eport an ew route for the effective synthesis of toluene from isoprene and acrolein, two reactants readily availablef rom biomass, through as imple two-step reaction. This route provides good selectivity compared to the pyrolysis strategy.…”

Cyanobacteria: Promising biocatalysts for sustainable chemical production

“…Cyanobacteria do not naturally encode an isoprene synthase, and integration of this gene from plants results in heterologous formation of isoprene which evaporates from the growth medium (78). Gao et al used in silico metabolic modelling to simulate flux and optimize carbon flow through the complete MEP pathway, leading to significantly increased production of isoprene (80). Engineering of the whole pathway represents a method to overcome the inherent regulation of native pathways that may limit product yields.…”