Cyanobacteria: Promising biocatalysts for sustainable chemical production

Knoot, C.J.; Ungerer, Justin; Wangikar, Pramod P.; Pakrasi, Himadri B.

doi:10.1074/jbc.r117.815886

Cited by 187 publications

(147 citation statements)

References 101 publications

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“…One of the reasons for this success is their large diversity in terms of morphology and physiology, which involves a large metabolic flexibility. These properties, their rather plane growth requirements and the already established genetic tools for their metabolic engineering, make cyanobacteria one of the most appealing class of phototrophic organisms to be employed for biotechnological applications (Knoot et al ). However, several hurdles have to be overcome in order to render the industrial exploitation of cyanobacteria economically feasible (Johnson et al ).…”

Section: Introductionmentioning

confidence: 99%

“…One of the reasons for this success is their Abbreviations --car, -carotene; Asx, astaxanthin; Can, canthaxanthin; Car, carotenoids; Chla, chlorophyll a; Ech, echinenone; Zea, zeaxanthin. large diversity in terms of morphology and physiology, which involves a large metabolic flexibility. These properties, their rather plane growth requirements and the already established genetic tools for their metabolic engineering, make cyanobacteria one of the most appealing class of phototrophic organisms to be employed for biotechnological applications (Knoot et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Non‐endogenous ketocarotenoid accumulation in engineeredSynechocystissp. PCC 6803

Menin

Santabarbara

Lami³

et al. 2019

Physiologia Plantarum

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The cyanobacterium Synechocystis sp. PCC 6803 is a model species commonly employed for biotechnological applications. It is naturally able to accumulate zeaxanthin (Zea) and echinenone (Ech), but not astaxanthin (Asx), which is the highest value carotenoid produced by microalgae, with a wide range of applications in pharmaceutical, cosmetics, food and feed industries. With the aim of finding an alternative and sustainable biological source for the production of Asx and other valuable hydroxylated and ketolated intermediates, the carotenoid biosynthetic pathway of Synechocystis sp. PCC 6803 has been engineered by introducing the 4,4′ β‐carotene oxygenase (CrtW) and 3,3′ β‐carotene hydroxylase (CrtZ) genes from Brevundimonas sp. SD‐212 under the control of a temperature‐inducible promoter. The expression of exogenous CrtZ led to an increased accumulation of Zea at the expense of Ech, while the expression of exogenous CrtW promoted the production of non‐endogenous canthaxanthin and an increase in the Ech content with a concomitant strong reduction of β‐carotene (β‐car). When both Brevundimonas sp. SD‐212 genes were coexpressed, significant amounts of non‐endogenous Asx were obtained accompanied by a strong decrease in β‐car content. Asx accumulation was higher (approximately 50% of total carotenoids) when CrtZ was cloned upstream of CrtW, but still significant (approximately 30%) when the position of genes was inverted. Therefore, the engineered strains constitute a useful tool for investigating the ketocarotenoid biosynthetic pathway in cyanobacteria and an excellent starting point for further optimisation and industrial exploitation of these organisms for the production of added‐value compounds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Non‐endogenous ketocarotenoid accumulation in engineeredSynechocystissp. PCC 6803

Menin

Santabarbara

Lami³

et al. 2019

Physiologia Plantarum

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“…During photoautotrophic growth of cyanobacteria, the primary MEP feeder molecules, G3P and Pyr, are in higher abundance than that of Acetyl-CoA [30]. Carbon flux through acetyl-CoA is greater during heterotrophic growth, such as during glycolysis, dark fermentation or oxidative respiration of glycogen or starch stores [15,33]. Each process entails a loss of CO 2 in the process of IPP and/or DMAPP synthesis [30].…”

Section: Reactants and Reactionsmentioning

confidence: 99%

“…In the light reactions of photosynthesis, ATP and NADPH are generated in a specific ratio, which may impact the efficiency of the isoprenoid biosynthetic pathway [15,36]. In the light reactions of photosynthesis, ATP and NADPH are generated in a specific ratio, which may impact the efficiency of the isoprenoid biosynthetic pathway [15,36].…”

Section: Reactants and Reactionsmentioning

confidence: 99%

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Engineering isoprene synthesis in cyanobacteria

Chaves

Melis

2018

FEBS Letters

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The renewable production of isoprene (Isp) hydrocarbons, to serve as fuel and synthetic chemistry feedstock, has attracted interest in the field recently. Isp (C H ) is naturally produced from sunlight, CO and H O photosynthetically in terrestrial plant chloroplasts via the terpenoid biosynthetic pathway and emitted in the atmosphere as a response to heat stress. Efforts to institute a high capacity continuous and renewable process have included heterologous expression of the Isp synthesis pathway in photosynthetic microorganisms. This review examines the premise and promise emanating from this relatively new research effort. Also examined are the metabolic engineering approaches applied in the quest of renewable Isp hydrocarbons production, the progress achieved so far, and barriers encountered along the way.

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