Engineering the chloroplast of Chlamydomonas reinhardtii to express the recombinant PfCelTOS-Il2 antigen-adjuvant fusion protein

Shamriz, Shabnam; Ofoghi, Hamideh

doi:10.1016/j.jbiotec.2017.12.015

Cited by 15 publications

(2 citation statements)

References 37 publications

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“…Examples include epitopes of the foot-and-mouth disease virus [138], the bacterium Staphylococcus aureus [139], and diabetes- [140] and atherosclerosis-associated autoantigens [141]. A series of works, instead, produced surface epitopes of the malariacausing agent Plasmodium falciparum [112,113,[142][143][144][145][146] that could elicit transmission-blocking antibodies in mice. Importantly, a major constraint of expressing viral antigens in algal chloroplasts is their susceptibility to unspecific proteolytic degradation [58].…”

Section: Microalgal Molecular Pharmingmentioning

confidence: 99%

Harnessing the Algal Chloroplast for Heterologous Protein Production

et al. 2022

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Photosynthetic microbes are gaining increasing attention as heterologous hosts for the light-driven, low-cost production of high-value recombinant proteins. Recent advances in the manipulation of unicellular algal genomes offer the opportunity to establish engineered strains as safe and viable alternatives to conventional heterotrophic expression systems, including for their use in the feed, food, and biopharmaceutical industries. Due to the relatively small size of their genomes, algal chloroplasts are excellent targets for synthetic biology approaches, and are convenient subcellular sites for the compartmentalized accumulation and storage of products. Different classes of recombinant proteins, including enzymes and peptides with therapeutical applications, have been successfully expressed in the plastid of the model organism Chlamydomonas reinhardtii, and of a few other species, highlighting the emerging potential of transplastomic algal biotechnology. In this review, we provide a unified view on the state-of-the-art tools that are available to introduce protein-encoding transgenes in microalgal plastids, and discuss the main (bio)technological bottlenecks that still need to be addressed to develop robust and sustainable green cell biofactories.

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Section: Microalgal Molecular Pharmingmentioning

confidence: 99%

Harnessing the Algal Chloroplast for Heterologous Protein Production

et al. 2022

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“…Among these candidates, expression of viral epitopes from Zika virus [ 115 ], avian influenza [ 116 ], human papillomavirus [ 117 ], hepatitis B [ 41 ], and human immunodeficiency virus (HIV) [ 118 ], as well bacterial proteins from Staphylococcus aureus [ 76 ] and Histophilus somni [ 119 ], are well studied. Regarding parasitic infections, proteins from Plasmodium falciparum that cause malaria are also expressed in microalgae [ 120 , 121 ]. Furthermore, microalgae are also used to produce monoclonal antibodies, hormones, cytokines, growth factors, immunotoxins, and proteins to prevent non-communicable diseases [ 122 , 123 , 124 , 125 ].…”

Section: Algal Biotechnology In Pharmaceutical Applicationsmentioning

confidence: 99%

Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae

et al. 2022

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During the last two decades, microalgae have attracted increasing interest, both commercially and scientifically. Commercial potential involves utilizing valuable natural compounds, including carotenoids, polysaccharides, and polyunsaturated fatty acids, which are widely applicable in food, biofuel, and pharmaceutical industries. Conversely, scientific potential focuses on bioreactors for producing recombinant proteins and developing viable technologies to significantly increase the yield and harvest periods. Here, viral-based vectors and transient expression strategies have significantly contributed to improving plant biotechnology. We present an updated outlook covering microalgal biotechnology for pharmaceutical application, transformation techniques for generating recombinant proteins, and genetic engineering tactics for viral-based vector construction. Challenges in industrial application are also discussed.

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Biorefinery potential of Dunaliella (Chlorophyta) for production of value‐added compounds

Sharma,

Singh,

Singh

2024

Biofuels Bioprod Bioref

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Dunaliella functions as a microscopic bioindustry that can capture atmospheric carbon dioxide and amass it into a range of economically viable compounds. Dunaliella‐based biorefineries have emerged as sustainable platforms for fostering a green economy and reducing carbon emissions. Dunaliella‐derived value‐added compounds are commercially important due to their application in the food, pharmaceutical, cosmeceutical, and nutraceutical sectors. The present study highlights the economic application of different Dunaliella species and the available sustainable technologies for the cultivation, harvesting, and extraction of bioactive compounds that have the potential for commercial scaleup. Their incorporation into biorefinery processes can reduce dependency on energy‐intensive and low‐yield processes.This review briefly discusses the technoeconomic analysis (TEA) of Dunaliella‐driven industrial approaches for the optimization of effective yield and determination of final production costs. The major algal biorefineries are operational in countries like Australia, China, India, Israel, Japan, Portugal, Spain and the USA. The authors have comprehensively deliberated different aspects of Dunaliella from physiological to industrial applications, meanwhile underscoring the existing knowledge gaps that impede the success of Dunaliella biorefineries. The current industrial methods being employed for biorefineries are limited to producing individual bioproducts, which may not be fully cost effective, making them less competitive in the global market. However, the analysis carried out can serve as a starting point and can guide stakeholders towards the future direction of Dunaliella‐based research and the circular bioeconomy.

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Engineering the chloroplast of Chlamydomonas reinhardtii to express the recombinant PfCelTOS-Il2 antigen-adjuvant fusion protein

Cited by 15 publications

References 37 publications

Harnessing the Algal Chloroplast for Heterologous Protein Production

Harnessing the Algal Chloroplast for Heterologous Protein Production

Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae

Biorefinery potential of Dunaliella (Chlorophyta) for production of value‐added compounds

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