Engineering Resistance to Raspberry Bushy Dwarf Virus

Martin, R.R.; Mathews, Helena

doi:10.17660/actahortic.2001.551.4

Cited by 13 publications

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“…RBDV is transmitted through pollen and seeds from infected plants and can spread rapidly through fields (2,5). Because of its pollen-and seed-borne nature, RBDV spreads during bloom.…”

mentioning

confidence: 99%

Comparison of Sugar, Acids, and Volatile Composition in Raspberry Bushy Dwarf Virus-Resistant Transgenic Raspberries and the Wild Type ‘Meeker’ (Rubus IdaeusL.)

Malowicki

Martín

Qian

2008

J. Agric. Food Chem.

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Raspberry bushy dwarf virus (RBDV) causes a significant reduction in yield and quality in raspberry and raspberry-blackberry hybrid. Genetic modifications were made to 'Meeker' red raspberries to impart RBDV resistance. The RBDV-resistant transgenic and wild type 'Meeker' plants were grown in Oregon and Washington, and the fruits were harvested in the 2004 and 2005 growing seasons. Year-to-year and site-to-site variations were observed for the degrees Brix and titratable acidity, with Oregon raspberries having slightly higher degrees Brix and lower titratable acidity than Washington raspberries. Twenty-nine volatile compounds were quantified using stir bar sorptive extraction (SBSE) paired with gas chromatography-mass spectrometry (GC-MS). There were very few differences in volatile concentrations between the transgenic varieties and the wild type 'Meeker'. Much larger variations were observed between sites and harvest seasons. Raspberries grown in Oregon appeared to have higher concentrations of delta-octalactone, delta-decalactone, geraniol, and linalool. Chiral analysis of alpha-ionone, alpha-pinene, linalool, terpinen-4-ol, delta-octalactone, and delta-decalactone demonstrated a much higher percentage of one isomer over the other, particularly alpha-ionone, alpha-pinene, delta-octalactone, and delta-decalactone, with more than 90% of one isomer, while a racemic mixture was observed for linalool. The isomeric analysis revealed very little variation between varieties, locations, or years. The flavor compounds tested in this study did not show any difference between the transgenic lines and the wild type 'Meeker' raspberry.

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“…RBDV is transmitted through pollen and seeds from infected plants and can spread rapidly through fields (2,5). Because of its pollen-and seed-borne nature, RBDV spreads during bloom.…”

mentioning

confidence: 99%

Comparison of Sugar, Acids, and Volatile Composition in Raspberry Bushy Dwarf Virus-Resistant Transgenic Raspberries and the Wild Type ‘Meeker’ (Rubus IdaeusL.)

Malowicki

Martín

Qian

2008

J. Agric. Food Chem.

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“…Developing molecular markers to identify RBDV-resistant genotypes could be used for screening seedlings prior to planting in the fi eld or for screening selections with other desired characteristics (Martin and Mathews 2001 ;Malowicki et al 2008a, b ) .…”

Section: Resistance To Diseasesmentioning

confidence: 99%

“…Martin's USDA-ARS lab in Oregon has developed resistant plants via transformation, but because of public and industry concerns with genetically modifi ed organisms (GMOs) these have never been released to producers (Martin and Mathews 2001 ;Martin et al 2004 ) .…”

Section: Resistance To Diseasesmentioning

confidence: 99%

“…This technology has been used to generate clones of 'Meeker' raspberry with resistance to RBDV through the incorporation of genetic constructs for the coat protein or movement protein of the virus (Martin and Mathews 2001 ) . Many of the transgenic plants had poor fruit set or other issues in the plants, but some selections were obtained with good resistance to the virus and potential for commercial development.…”

Section: Transgenicsmentioning

confidence: 99%

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Raspberry

Kempler

Hall²,

Finn

2011

Fruit Breeding

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The red raspberry, Rubus idaeus L., is a valuable crop that has recently increased in production, generating a large interest in commercial ventures and in research. Traditionally, most of the crop has been sold to processors for freezing, jam production, canning, juice and fl avorings for ice cream, yogurt, and other products, but in recent years fresh market production has increased and become a very important sector of this industry. There has been an increased interest in black, purple, and Arctic raspberries because of their high nutraceutical value. R. idaeus, a diploid (2 n = 14), is included in the Idaeobatus and is the most important commercial species in this subgenus. The fl owers are hermaphroditic; however, in some cases, they are unisexual, especially among wild species. Domestication of raspberries is comparably recent as it occurred less than 500 years ago. Red raspberries are widely distributed in all temperate regions of Europe, Asia, and North America with the greatest diversity in China. Enriching the cultivated gene pool by incorporating the unique genetics from wild germplasm to meet the challenges that lie ahead is desired. Breeding goals are the improvement of fruit quality which includes selection for better preharvest hanging ability and postharvest shelf life and processed quality. Resistance to heat and cold and resistance to pests and disease are also important, as well as large fruit size, good presentation, and ease of harvest. Fruit color of the newer cultivars varies from very dark red to a light orange-red and there has become a tradition of cultivar selection (eds.), Fruit Breeding, Handbook of Plant Breeding 8, DOI 10.1007/978-1-4419-0763-9_8, © Springer Science+Business Media, LLC 2012 264 C. Kempler et al.specifi cally for processing or for fresh market. There are now approximately 50 active raspberry breeding programs in 26 countries, mostly in Europe and North America. Use of molecular markers for genetic studies and mapping is referenced; however, in this crop, it is at an early stage with only a few genes mapped.

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“…'Meeker' red raspberry has been developed that contain several different RBDV constructs including: nontranslatable RNA, coat protein and three different mutated movement protein constructs (Martin et al, 2001). The objective of this study was to determine if the volatile composition of RBDV-resistant lines varies from wild-type 'Meeker' raspberry.…”

Section: Introductionmentioning

confidence: 99%

Fruit Quality of Transgenic 'Meeker' Red Raspberry With Resistance to Raspberry Bushy Dwarf Virus

Malowicki¹,

Qian²,

Martin³

2008

Acta Hortic.

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Raspberry bushy dwarf virus (RBDV) causes significant reduction in yield and crumbly fruit in raspberries, blackberries and raspberry-blackberry hybrids; the only means of control is through development of resistant cultivars. Genetic modifications have been made to 'Meeker' red raspberries in an effort to develop resistance to RBDV while maintaining the desirable fruit characteristics of 'Meeker' including sweetness, aroma profile and machine harvestability. Transgenic and wildtype 'Meeker' plants were grown in Oregon and Washington to compare the fruit quality under different climatic conditions over several years and to evaluate field resistance to RBDV. After six field seasons, only one of the transgenic lines was still completely free of RBDV under extreme disease pressure. The wild-type 'Meeker' plants in the same plots were 100% infected (202/202) after three field seasons. This transgenic line along with four other lines that were less than 100% infected after six field seasons was not infected with RBDV by grafting after three attempts. Fruit quality indicators such as °Brix, titratable acidity and sugar and organic acid profiles were performed on fruit from wild-type and five transgenic lines of 'Meeker' raspberries grown in Oregon and Washington for the years 2004 and 2005. Significant differences (p<0.05) were found between the years for all tests performed. °Brix and titratable acidity were also significantly affected by locations with berries grown in Washington having higher acid and lower °Brix than Oregon. The RBDV-resistant transgenic lines were similar to the wild-type 'Meeker' red raspberry. Experimental results showed that fruit quality is affected by season and growing environments. Additionally, the five transgenic lines behaved similarly to the wild-type 'Meeker' raspberry in yield. These results demonstrated that the transgenic RBDV-resistant lines of 'Meeker' could serve as a replacement for the wild-type 'Meeker' red raspberry provided that the fruit was accepted in the marketplace.

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Engineering Resistance to Raspberry Bushy Dwarf Virus

Cited by 13 publications

References 0 publications

Comparison of Sugar, Acids, and Volatile Composition in Raspberry Bushy Dwarf Virus-Resistant Transgenic Raspberries and the Wild Type ‘Meeker’ (Rubus IdaeusL.)

Comparison of Sugar, Acids, and Volatile Composition in Raspberry Bushy Dwarf Virus-Resistant Transgenic Raspberries and the Wild Type ‘Meeker’ (Rubus IdaeusL.)

Raspberry

Fruit Quality of Transgenic 'Meeker' Red Raspberry With Resistance to Raspberry Bushy Dwarf Virus

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