Engineering probiotics as living diagnostics and therapeutics for improving human health

Zhou, Zhao; Chen, Xin; Sheng, Huakang; Shen, Xiaolin; Sun, Xinxiao; Yan, Yajun; Jia, Wentao; Yuan, Qipeng

doi:10.1186/s12934-020-01318-z

Cited by 75 publications

(59 citation statements)

References 104 publications

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“…Bacteria can be used as a diagnostic device through engineering techniques that make the bacteria sense a specific molecule in the human body and consequently produce a signal [106]. For instance, Danino et al [107] developed a probiotic-based diagnostic that is orally administered.…”

Section: Probiotics Provide a Better Diagnosis Of Cervical Cancermentioning

confidence: 99%

Anti-tumor activities of probiotics in cervical cancer

et al. 2020

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Cervical cancer is considered as an important malignancy among women worldwide. Currently-used treatments of cervical cancer are reported to be cytotoxic for patients. Moreover, these therapies have shown some side effects which can negatively affect the lives of women suffering from this cancer. Therefore, there is need for anti-tumor agents that are less toxic than common therapeutic drugs. Besides, applying agents for preventing or reducing the side effects of cervical cancer therapies can be effective in improving the life quality of cervical cancer patients. Studies have shown that probiotics have several effects on biological processes. One of the most prominent aspects in which probiotics play a role is in the field of cancer. There are multiple studies which have focused on the functions of probiotics in diagnosis, prevention, or treatment of cancer. Besides their direct anti-tumor activities, probiotics can be used as an additional agent for enhancing or modulating other diagnostic and therapeutic methods. Herein, the effects of probiotics on cervical cancer cells are discussed, which may be useful in the prevention and treatment of this cancer. We review the studies concerned with the roles of probiotics in modulating and reducing the gastrointestinal adverse effects caused by cervical cancer therapies. Furthermore, we cover the investigations focusing on the combination of probiotics with other drugs for diagnosis or treatment of cervical cancer.

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Section: Probiotics Provide a Better Diagnosis Of Cervical Cancermentioning

confidence: 99%

Anti-tumor activities of probiotics in cervical cancer

et al. 2020

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“…This inhibition impacts bacterial pathogenicity in vitro and in vivo but not cell growth [ 250 ]. Because of their function to sense the host conditions, TCSs together with transcriptional regulators are central actors in the design of engineered probiotics [ 251 , 252 ]. As a proof of concept to treat bacterial infection, the probiotic E. coli Nissle has been engineered to sense tetrathionate, a molecule produced in the inflamed gut during Salmonella infection, through the TtrS/R TCS.…”

Section: Antimicrobial Strategies Related With Tcs and Associated mentioning

confidence: 99%

Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection

Piattelli

Peltier

Soutourina

2020

Genes

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The ability of pathogenic bacteria to stably infect the host depends on their capacity to respond and adapt to the host environment and on the efficiency of their defensive mechanisms. Bacterial envelope provides a physical barrier protecting against environmental threats. It also constitutes an important sensory interface where numerous sensing systems are located. Signal transduction systems include Two-Component Systems (TCSs) and alternative sigma factors. These systems are able to sense and respond to the ever-changing environment inside the host, altering the bacterial transcriptome to mitigate the impact of the stress. The regulatory networks associated with signal transduction systems comprise small regulatory RNAs (sRNAs) that can be directly involved in the expression of virulence factors. The aim of this review is to describe the importance of TCS- and alternative sigma factor-associated sRNAs in human pathogens during infection. The currently available genome-wide approaches for studies of TCS-regulated sRNAs will be discussed. The differences in the signal transduction mediated by TCSs between bacteria and higher eukaryotes and the specificity of regulatory RNAs for their targets make them appealing targets for discovery of new strategies to fight against multi-resistant bacteria.

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“…For example, an engineered Lactococcus lactis has been used to treat type II diabetes [5] , [6] . Additionally, an engineered Escherichia coli Nissle 1917 ( E. coli ) is being tested in a phase II clinical trial (NCT04534842) based on its ability to breakdown phenylalanine to treat phenylketonuria after demonstrating efficacy in animal studies [7] . Further attempts are made to combat pathogenic bacteria, such as Pseudomonas aeruginosa, Vibrio cholerae and Salmonella using engineered E. coli Nissle strains [7] .…”

Section: Introductionmentioning

confidence: 99%