Engineering of microbial cells for L-valine production: challenges and opportunities

Abstract

L-valine is an essential amino acid that has wide and expanding applications with a suspected growing market demand. Its applicability ranges from animal feed additive, ingredient in cosmetic and special nutrients in pharmaceutical and agriculture fields. Currently, fermentation with the aid of model organisms, is a major method for the production of L-valine. However, achieving the optimal production has often been limited because of the metabolic imbalance in recombinant strains. In this review, the constrains in L-valine biosynthesis are discussed first. Then, we summarize the current advances in engineering of microbial cell factories that have been developed to address and overcome major challenges in the L-valine production process. Future prospects for enhancing the current L-valine production strategies are also discussed.

Proteomic Analysis of Pleurotus ostreatus Grown on Glucose and Xylose Mixtures in Submerged Fermentation Provides Insights into Differentiated Mycelial Composition

“…BCAAs are synthesized by fungi and are essential for the human diet as they are key components of human and animal nutrition and need to be obtained from daily nutrition. Also, they play a role in protein synthesis and muscle metabolism, with increased BCAAs concentrations to indicatingdecreased muscle soreness and prevention of muscle breakdown during exercise [40] Their applications are related to food, feed, cosmetic and pharmaceutical industries [41]. The BCAA metabolic pathway is well characterized in Saccharomyces cerevisiae, in which the transcriptional regulation is conducted by the Zn(II)2Cys6 transcription factors LEU3.…”

The biological functions and metabolic pathways of valine in swine

“…coli KCCM 80159 was added to the diet, which was safe for consumers, users and the environment, and the valine produced by fermentation also was used as an effective source of nutritionally essential amino acid L -valine in non-ruminant animals [ 170 , 171 ]. However, considering that competitive pathways have the interdependency and metabolic burdens in engineered strains, it is a challenge to optimize high valine production for a single bacterial strain [ 172 ]. In the coming period, high-throughput biosensor screening would be instrumental in high-yielding valine producer strains [ 32 , 172 ].…”

“…However, considering that competitive pathways have the interdependency and metabolic burdens in engineered strains, it is a challenge to optimize high valine production for a single bacterial strain [ 172 ]. In the coming period, high-throughput biosensor screening would be instrumental in high-yielding valine producer strains [ 32 , 172 ].…”

Green and Sustainable Carbon Dioxide Capture in Aqueous l-Valine: Experimental Studies on Process Efficiency and Gas Uptake for Industrial Applications

“…The amino acid (L-valine) has been chosen for this specific study as it is relatively less expensive than most of amines and amino acids and is produced in large quantities due to its high demand for application in pharmaceuticals, cosmetics, food supplements etc. 47 In industries, L-valine production can be achieved through a biobased process utilizing the microorganism Corynebacterium glutamicum in fermentation. 48 This sustainable approach holds promise for ecofriendly and efficient manufacturing of L-valine.…”

“…To understand the standalone performance of aqueous amino acids, this work reports for the first time to the best of our knowledge, pure (aq amino acid) to capture CO 2 by stepwise injection method in a laboratory-scale setup of 100 mL. The amino acid ( l -valine) has been chosen for this specific study as it is relatively less expensive than most of amines and amino acids and is produced in large quantities due to its high demand for application in pharmaceuticals, cosmetics, food supplements etc . In industries, l -valine production can be achieved through a biobased process utilizing the microorganism Corynebacterium glutamicum in fermentation .…”