Engineering of erythropoietin receptor for use as an affinity ligand

Hatayama, Kouta; Ide, T.

doi:10.1016/j.pep.2015.03.005

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…A complication however has been the substantially different affinities of the target analytes towards the EPOR, an aspect that has initiated further engineering of an improved EPOR receptor specifically designed for its application in affinity purification resins. [107] As recently corroborated by different studies, it is commonly accepted that EPO enhances athletic performance by routes including haematopoietic but also muscle-tissue specific [108] and placebo [109] effects, albeit adaptational changes in muscle morphology by EPO have also been questioned. [110] Hence, in the light of the labor-and cost-intensiveness of EPO analyses and the need for comprehensive EPO analyses, fast initial testing options have been desirable.…”

Section: Peptide Hormones Growth Factors and Related Substances Erytmentioning

confidence: 98%

“…erythropoietin‐mimetic peptide 1 (EMP 1), BB68, and peginesatide) were isolated from human urine and, following non‐enzymatic cysteine‐specific hydrolysis, analyzed at an LOD of 0.25 ng/mL, suggesting the principle applicability of EPOR‐based sample preparation strategies to doping controls. A complication however has been the substantially different affinities of the target analytes towards the EPOR, an aspect that has initiated further engineering of an improved EPOR receptor specifically designed for its application in affinity purification resins …”

Section: Peptide Hormones Growth Factors and Related Substancesmentioning

confidence: 99%

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Annual banned‐substance review: analytical approaches in human sports drug testing

Thevis

Kuuranne

Walpurgis

et al. 2016

Drug Testing and Analysis

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The aim of improving anti-doping efforts is predicated on several different pillars, including, amongst others, optimized analytical methods. These commonly result from exploiting most recent developments in analytical instrumentation as well as research data on elite athletes' physiology in general, and pharmacology, metabolism, elimination, and downstream effects of prohibited substances and methods of doping, in particular. The need for frequent and adequate adaptations of sports drug testing procedures has been incessant, largely due to the uninterrupted emergence of new chemical entities but also due to the apparent use of established or even obsolete drugs for reasons other than therapeutic means, such as assumed beneficial effects on endurance, strength, and regeneration capacities. Continuing the series of annual banned-substance reviews, literature concerning human sports drug testing published between October 2014 and September 2015 is summarized and reviewed in reference to the content of the 2015 Prohibited List as issued by the World Anti-Doping Agency (WADA), with particular emphasis on analytical approaches and their contribution to enhanced doping controls.

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