Engineering of CRISPR/Cas9‐mediated potyvirus resistance in transgene‐free <i>Arabidopsis</i> plants

Pyott, Douglas E.; Sheehan, Emma; Molnár, Attila

doi:10.1111/mpp.12417

Cited by 353 publications

(200 citation statements)

References 61 publications

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“…A. thaliana mutant plants of these translation initiation factors exhibited resistance to Turnip mosaic virus (TuMV) (Lellis et al, 2002). Therefore these targets were mutated with the CRISPR/Cas9 system, in order to develop virus resistant plants (Chandrasekaran et al, 2016; Pyott et al, 2016). The utility of CRISPR/Cas9 technology for generating novel genetic resistance to the potyvirus TuMV was demonstrated in A. thaliana by deletion of a host factor, eIF(iso)4E , which is strictly required for viral survival (Pyott et al, 2016).…”

Section: Engineering Crispr/cas9-based Resistance Against Rna Virusesmentioning

confidence: 99%

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

et al. 2016

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Plant viruses infect many economically important crops, including wheat, cotton, maize, cassava, and other vegetables. These viruses pose a serious threat to agriculture worldwide, as decreases in cropland area per capita may cause production to fall short of that required to feed the increasing world population. Under these circumstances, conventional strategies can fail to control rapidly evolving and emerging plant viruses. Genome-engineering strategies have recently emerged as promising tools to introduce desirable traits in many eukaryotic species, including plants. Among these genome engineering technologies, the CRISPR (clustered regularly interspaced palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) system has received special interest because of its simplicity, efficiency, and reproducibility. Recent studies have used CRISPR/Cas9 to engineer virus resistance in plants, either by directly targeting and cleaving the viral genome, or by modifying the host plant genome to introduce viral immunity. Here, we briefly describe the biology of the CRISPR/Cas9 system and plant viruses, and how different genome engineering technologies have been used to target these viruses. We further describe the main findings from recent studies of CRISPR/Cas9-mediated viral interference and discuss how these findings can be applied to improve global agriculture. We conclude by pinpointing the gaps in our knowledge and the outstanding questions regarding CRISPR/Cas9-mediated viral immunity.

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Section: Engineering Crispr/cas9-based Resistance Against Rna Virusesmentioning

confidence: 99%

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

et al. 2016

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“…-the integration of CRISPR-coding sequence in the host plant genome that targets and interferes with the virus genome once it is incorporated in the plant: the aim is to establish a CRISPR-like immune system in the host genome [7,14] and -the induction of a CRISPR-mediated targeted mutation in the host plant genome that will confer improved virus resistance traits [9].…”

Section: Inhibition Of Tomato Fruit Ripeningmentioning

confidence: 99%

Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture

Ricroch

Clairand

Harwood

2017

Emerging Topics in Life Sciences

195

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Initially discovered in bacteria and archaea, CRISPR-Cas9 is an adaptive immune system found in prokaryotes. In 2012, scientists found a way to use it as a genome editing tool. In 2013, its application in plants was successfully achieved. This breakthrough has opened up many new opportunities for researchers, including the opportunity to gain a better understanding of plant biological systems more quickly. The present study reviews agricultural applications related to the use of CRISPR systems in plants from 52 peerreviewed articles published since 2014. Based on this literature review, the main use of CRISPR systems is to achieve improved yield performance, biofortification, biotic and abiotic stress tolerance, with rice (Oryza sativa) being the most studied crop.

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“…One of the outstanding points of genome editing in terms of its application to crop breeding is that the original transgenes for genome editing can be removed via segregation after editing. Recently, the CRISPR/Cas9 system was used to establish eIFiso4E-deficient Arabidopsis mutants that were free from transgenes and exhibited recessive resistance to TuMV (Pyott et al, 2016). More importantly, the CRISPR/Cas9 system was also applied to cucumber ( Cucumis sativus ) to disrupt the eIF4E gene, and the non-transgenic, eIF4E-deficient plant lines were resistant to the cucumber vein yellowing virus (CVYV; Ipomovirus ) and two potyviruses (Chandrasekaran et al, 2016).…”

Section: Strategies For Improving the Genetic Resources For Recessivementioning

confidence: 99%

Recessive Resistance to Plant Viruses: Potential Resistance Genes Beyond Translation Initiation Factors

et al. 2016

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The ability of plant viruses to propagate their genomes in host cells depends on many host factors. In the absence of an agrochemical that specifically targets plant viral infection cycles, one of the most effective methods for controlling viral diseases in plants is taking advantage of the host plant’s resistance machinery. Recessive resistance is conferred by a recessive gene mutation that encodes a host factor critical for viral infection. It is a branch of the resistance machinery and, as an inherited characteristic, is very durable. Moreover, recessive resistance may be acquired by a deficiency in a negative regulator of plant defense responses, possibly due to the autoactivation of defense signaling. Eukaryotic translation initiation factor (eIF) 4E and eIF4G and their isoforms are the most widely exploited recessive resistance genes in several crop species, and they are effective against a subset of viral species. However, the establishment of efficient, recessive resistance-type antiviral control strategies against a wider range of plant viral diseases requires genetic resources other than eIF4Es. In this review, we focus on recent advances related to antiviral recessive resistance genes evaluated in model plants and several crop species. We also address the roles of next-generation sequencing and genome editing technologies in improving plant genetic resources for recessive resistance-based antiviral breeding in various crop species.

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Engineering of CRISPR/Cas9‐mediated potyvirus resistance in transgene‐free Arabidopsis plants

Cited by 353 publications

References 61 publications

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture

Recessive Resistance to Plant Viruses: Potential Resistance Genes Beyond Translation Initiation Factors

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