Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture

Ricroch, Agnés; Clairand, Pauline; Harwood, Wendy

doi:10.1042/etls20170085

Cited by 196 publications

(87 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The same cautious optimism seems to prevail also among some other EU countries. Ricroch et al (2017) listed 52 peer-reviewed articles published in 2014-2017, all applying CRISPR systems in plants within an agricultural context. They found that EU (9 studies, carried out in Austria, Belgium, France, Germany, Hungary, Sweden and the United Kingdom) is, together with China (22 studies) and United States (10 studies) among the regions in the world with most published proof-of-concept studies for CRISPR applications within this context.…”

Section: Discussionmentioning

confidence: 99%

“…The most widely known so far is the clustered regularly interspaced short palindromic repeats (CRISPR) system, including a CRISPR associated (Cas) endonuclease protein, while zinc finger nucleases, transcription activator‐like effector nucleases (TALENs) and the nucleic acid‐based oligonucleotide‐directed mutagenesis (ODM) are also routinely applied. It is beyond the scope of this review to describe the genome editing techniques in detail; however, several recent reviews are available on various aspects, such as the technical details (NTWG , Chen and Gao , Abdallah et al , Hilscher et al , HLG SAM , entire issue of Plant Biotechnology Journal ), specifically on CRISPR systems (Bortesi and Fischer , Ding et al , Paul III and Qi , Samanta et al , Stella and Montoya , Arora and Narula , Volpi e Silva and Patron , Yin et al ), applications in plants (Brinegar et al , Hilscher et al , Ricroch et al , Zhang et al ), as well as the historical development (Songstad et al ) and comparisons with other breeding techniques (Georges and Ray ).…”

Section: Introductionmentioning

confidence: 99%

“…The most widely known so far is the clustered regularly interspaced Abbreviations -CJEU, Court of Justice of the European Union; CRISPR, clustered regularly interspaced short palindromic repeats; EC, European Commission; EFSA, European Food Safety Authority; GMO, genetically modified organism; HLG SAM, high-level group of Scientific Advice Mechanism; JRC, Joint Research Centre; NCA, National Competent Authority; NPBTs, new plant breeding techniques; NTWG, new techniques working group; ODM, oligonucleotide-directed mutagenesis; RTDS, Rapid Trait Development System; SBA, Swedish Board of Agriculture; SDN, site-directed nuclease; TALENs, transcription activator-like effector nucleases; ZFNs, zinc finger nucleases. Paul III and Qi 2016, Samanta et al 2016, Stella and Montoya 2016, Arora and Narula 2017, Volpi e Silva and Patron 2017, Yin et al 2017, applications in plants (Brinegar et al 2017, Hilscher et al 2017, Ricroch et al 2017, Zhang et al 2017, as well as the historical development (Songstad et al 2017) and comparisons with other breeding techniques (Georges and Ray 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Swedish policy approach to directed mutagenesis in a European context

Eriksson

2018

Physiologia Plantarum

View full text Add to dashboard Cite

This review describes the Swedish approach to directed mutagenesis in plants and puts it in a comparative European perspective. Directed mutagenesis is accomplished by a number of genome editing techniques; however, the legal status of these techniques and their resulting products is uncertain in the European Union (EU) as there is no political consensus on whether or not these should be regulated as genetically modified organisms (GMOs). A number of cases have developed over the past few years, putting the GMO regulatory framework to the test. These include oilseed rape developed by oligonucleotide-directed mutagenesis, Arabidopsis developed by clustered regularly interspaced short palindromic repeat-Cas9, and the case on mutagenesis for which the French Court requested a preliminary ruling from the Court of Justice of the EU. In this review, the involvement of the Swedish Government and governmental authorities in these cases is described and compared with that of other EU member states and/or EU entity statements and reports. Various approaches to the definition of recombinant nucleic acids are also discussed, as this is crucial for the EU GMO definition thus affecting the legal status of products developed by directed mutagenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Swedish policy approach to directed mutagenesis in a European context

Eriksson

2018

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Parasitic weed resistance based on the editing of specific target genes using CRISPR/Cas9, can be easily applied to other susceptible crops and this method could be effective with other host plant, to develop parasite resistance. In addition, the host plants are not considered genetically modified organisms due to lack of any foreign DNA sequences in the mutated host plants 49 . One of the major challenges associated with CRISPR/Cas9 system is off-target cleavage due to non-specificity of the selected sgRNA with other few base pair mismatched target site within the genome 50 .…”

Section: Discussionmentioning

confidence: 99%

Mutagenesis of two homologs ATP Binding cassette protein in tomato by CRISPR/Cas9 provide resistance against the plant parasite Phelipanche aegyptiaca

Bari

Nassar

Meir

et al. 2019

Preprint

View full text Add to dashboard Cite

SUMMARYPlant parasitic weed Phelipanche aegyptiaca and Orobanche spp. are obligate plant parasites that cause heavy damage to agricultural crop plants. Germination of the parasite seeds require exposure to specific chemical known as strigolactone [SL]. The plant hormone SL is derived from plant carotenoids via cleavage by CCD7 and CCD8 enzymes and exuded by the host roots to the rhizosphere. Here, we provide evidence that CRISPR/Cas9 mediated targeted mutagenesis of two homologues ATP Binding cassette (ABC) protein in tomato (Solyc08g067610 and Solyc08g067620), significantly reduced the germination of the parasitic weed P.aegyptiaca. Constructs harboring specific single guide RNA were prepared and targeted against conserved region in the above tomato genes (Solyc08g067610 and Solyc08g067620). Selected T0-mutated tomato plants showed different type of deletions at both locuses. Furthermore, genotype analysis of T1 plants showed that the introduced mutations stably inherited to next generation with no identified off-targets. Mutated tomato lines, showed more branching, enhanced growth of axillary buds, reduced length of primary stems and significantly reduced development of the parasitic weed P. aegyptiaca as compared to wild type plants. Moreover, in ABCCas9 mutants the expression level of the genes (CCD8 and MAX1) related to SL biosynthesis were decreased, without alteration in the root extract orobanchol [SL] as compared to control plants. Development of genetic resistance based on genome editing of host key genes are novel approaches for enhancing host-parasite resistance. The current study offers insights into ABC protein homologs and mutagenesis of ABC protein that could be used in the development of efficient method to reduce parasitic weed germination.

show abstract

“…Components of bacterial CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) systems for adaptive immunity have been repurposed for engineering the genomes of eukaryotic organisms (1)(2)(3). These molecular tools have been rapidly and successfully applied in many organisms, including plants (for recent reviews see Gao, 2018;Ricroch et al, 2017;Yin et al, 2017), primarily due to the ease at which they can be programmed to recognise new genomic targets. The majority of plant genome engineering studies have utilised Cas9 (CRISPR Associated Protein 9) from Streptococcus pyogenes, a monomeric nuclease found in the Type II CRISPR system of that species.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Efficiency and Specificity of CRISPR-Associated (Cas) Nucleases in Plants: An Expanded Toolkit for Precision Genome Engineering

Raitskin

Schudoma

West

et al. 2018

Preprint

View full text Add to dashboard Cite

Repeats) systems for adaptive immunity have become widely used for plant genome engineering, both to investigate gene functions and to engineer desirable traits. A number of different Cas (CRISPRassociated) nucleases are now used but, as most studies performed to date have engineered different targets using a variety of plant species and molecular tools, it has been difficult to draw conclusions about the comparative performance of different nucleases. Due to the time and effort required to regenerate engineered plants, efficiency is critical. In addition, there have been several reports of mutations at sequences with less than perfect identity to the target. While in some plant species it is possible to remove these so-called 'off-targets' by backcrossing to a parental line, the specificity of genome engineering tools is important when targeting specific members of closely-related gene families, especially when recent paralogues are co-located in the genome and unlikely to segregate. Specificity is also important for species that take years to reach sexual maturity or that are clonally propagated. Here, we directly compare the efficiency and specificity of Cas nucleases from different bacterial species together with engineered variants of Cas9. We find that the nucleotide content correlates with efficiency and that Cas9 from Staphylococcus aureus is comparatively most efficient at inducing mutations. We also demonstrate that 'high-fidelity' variants of Cas9 can reduce off-target mutations in plants. We present these molecular tools as standardised DNA parts to facilitate their re-use.

show abstract

Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture

Cited by 196 publications

References 64 publications

The Swedish policy approach to directed mutagenesis in a European context

The Swedish policy approach to directed mutagenesis in a European context

Mutagenesis of two homologs ATP Binding cassette protein in tomato by CRISPR/Cas9 provide resistance against the plant parasite Phelipanche aegyptiaca

Comparison of Efficiency and Specificity of CRISPR-Associated (Cas) Nucleases in Plants: An Expanded Toolkit for Precision Genome Engineering

Contact Info

Product

Resources

About