Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications

Sailakshmi, G.; Mitra, Tapas; Gnanamani, A.

doi:10.1186/2194-0517-2-11

Cited by 31 publications

(18 citation statements)

References 44 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 3 shows that in the spectrum of chitosan/gelatin, C¼O groups of gelatin adsorbed with N-H groups of chitosan resulting in strong hydrogen bonds, leading to a far more miscibile chitosan/gelatin component. 24 As shown in Figure 4(b), the characteristic absorption bands of gelatin were observed at 1640 cm À1 (amide I), 1534 cm À1 (amide II), and 1234 cm À1 (amide III), respectively, while the peak at 1130 cm À1 was assigned to chitosan for its saccharine structure. Moreover, as shown in Figure 4(a), TPU has characteristic absorption bands at 3349 cm À1 (N-H), 2926 cm À1 (C-H), 1725 cm À1 (C¼O), 1217 cm À1 (C-O), and 1528 cm À1 assigned to the characteristic absorption of benzene.…”

Section: Resultsmentioning

confidence: 91%

Fabrication and characterization of chitosan/gelatin/thermoplastic polyurethane blend nanofibers

Gharaie

Habibi

Nazockdast

2018

Journal of Textiles and Fibrous Materials

View full text Add to dashboard Cite

Polymer blending is a method to provide nanocomposite nanofibers with improved strength and minimal defects. Chitosan exhibits biocompatibility, biodegradability, antimicrobial activity, and wound healing properties. A combination of gelatin and thermoplastic polyurethane (TPU) blends was explored as a means to improve the morphological deficiencies of chitosan nanofibers and facilitate its electrospinnability. The morphology of the electrospun chitosan, chitosan/ gelatin, and chitosan/gelatin/TPU blend nanofibers were characterized using scanning electron microscopy (SEM), while the miscibility and thermal behavior of the blends were determined using differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy/attenuated total reflectance (FTIR/ATR). The optimum results were achieved in blend with 3 wt% chitosan, 8 wt% gelatin, and 5 wt% TPU, which resulted nanofibers with a mean diameter of 100.6 nm + 17.831 nm.

show abstract

Section: Resultsmentioning

confidence: 91%

Fabrication and characterization of chitosan/gelatin/thermoplastic polyurethane blend nanofibers

Gharaie

Habibi

Nazockdast

2018

Journal of Textiles and Fibrous Materials

View full text Add to dashboard Cite

show abstract

“…The higher endothermic peak suggests that the DLS has high stability in high-temperature environments. 44…”

Section: Thermal Stabilitymentioning

confidence: 99%

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Li¹,

Wang²,

Yang³

et al. 2018

IJN

View full text Add to dashboard Cite

BackgroundAn ideal wound dressing should exhibit good biocompatibility, minimize pain and infection, absorb excess exudates, and maintain a moist environment. However, few clinical products meet all these needs. Therefore, the aim of this study was to fabricate a multifunctional double layer nanofibrous scaffolds (DLS) as a potential material for wound dressing.Materials and methodsThe scaffold was formed from mupirocin and lidocaine hydrochloride homogeneously incorporated into polycaprolactone as the first layer of scaffolds and chitosan as the second layer of scaffolds nanofibers through electrospinning. The fabricated nanofibrous scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, and measurement of swelling ratio, contact angle, drug release, and mechanical properties. Furthermore, antibacterial assessment, live/dead cell assays, and MTT assays were used to investigate the antibacterial activity and cytotoxicity of the nanofibrous scaffolds.ResultsThe morphology of the nanofibrous scaffolds was studied by scanning electron microscopy, showing successful nanofibrous scaffolds. Fourier transform infrared spectroscopy demonstrated the successful incorporation of the material used to produce the produced nanofibrous scaffolds. Thermal studies with thermogravimetric analysis and differential scanning calorimetry indicated that the DLS had high thermal stability. The DLS also showed good in vitro characteristics in terms of improved swelling ratio and contact angle. The mechanical results revealed that the DLS had an improved tensile strength of 3.88 MPa compared with the second layer of scaffold (2.81 MPa). The release of drugs from the scaffold showed different profiles for the two drugs. Lidocaine hydrochlo ride exhibited an initial burst release (66% release within an hour); however, mupirocin exhibited only a 5% release. Furthermore, the DLS nanofibers displayed highly effective antibacterial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa and were nontoxic to fibroblasts.ConclusionThe fabricated DLS exhibited excellent hydrophilicity, cytocompatibility, sustained drug release, and antibacterial activity, which are favorable qualities for its use as a multifunctional material for wound dressing applications.

show abstract

“…36,37 Mannitol is the sugar alcohol formed from the hexose sugar mannose and is commonly used as food ingredients. Since sebacic acid undergo cellular metabolism to form the TCA intermediate succinate, its use in cardiac tissue engineering hydrogels offers a safer application.…”

Section: Resultsmentioning

confidence: 99%

Hybrid amphiphilic bimodal hydrogels having mechanical and biological recognition characteristics for cardiac tissue engineering

Finosh

Jayabalan

2015

RSC Adv.

View full text Add to dashboard Cite

Tissue engineering strategies rely on the favourable microniche scaffolds for 3D cell growth. For cardiac tissue engineering, a biodegradable hydrogel has to meet the essential requirements viz. adequate strength, compatibility of degradation products to the host tissue, maintenance of cellular viability and differentiation, favouring cell integration, controlled degradation of scaffold commensurate with the contractile function under ischemic conditions of the injured heart. In this work, an attempt is made to explore some of these stringent and diagonally opposite requirements. Hybrid amphiphilic bimodal hydrogels having mechanical and biological recognition characteristics were developed using graft comacromer of alginate-poly(mannitol fumarate-co-sebacate). Alginate was graft copolymerized with poly(mannitol fumarate-co-sebacate) macromer (HT-MFS). The resultant comacromer was crosslinked with PEGDA and DEGDMA to form two bimodal hydrogel scaffolds. Both hydrogels exhibited better physiochemical and mechanical properties and supported long-term cell viability under static and dynamic conditions. Laser scanning confocal microscopy Z-stacking evaluations showed infiltration of FDA-stained L929 fibroblasts in the interstices of the hydrogels with appreciable depth. The hydrogel based on PEGDA promoted cell growth to an extent of 98 mm when compared to that of DEGDMA based hydrogel with 52 mm. These hydrogels supported the co-culture of fibroblasts and cardiomyoblasts and provided a better microniche for the cells as evident by the viability and cell cycle progression analyses. The favourable cellular responses of these hydrogels are attributed to the inherent biological recognition characteristics. On comparing the two hydrogels, the PEGDA-based hydrogel was superior to its DEGDMA counterpart due to the higher hydrophilicity of the former. The PEGDA-based hydrogel is a promising candidate for cardiac tissue engineering.

show abstract

Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications

Cited by 31 publications

References 44 publications

Fabrication and characterization of chitosan/gelatin/thermoplastic polyurethane blend nanofibers

Fabrication and characterization of chitosan/gelatin/thermoplastic polyurethane blend nanofibers

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Hybrid amphiphilic bimodal hydrogels having mechanical and biological recognition characteristics for cardiac tissue engineering

Contact Info

Product

Resources

About