Engineering microbes for isoprene production

Ye, Lidan; Lv, Xiaomei; Yu, Hongwei

doi:10.1016/j.ymben.2016.07.005

Cited by 81 publications

(92 citation statements)

References 109 publications

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“…4A). Control of metabolic flux through the mevalonate pathway is of paramount importance for prevention of various diseases (Akula et al, 2016;Frenkel et al, 2000), and for the development of biobased production of pharmaceuticals, food additives, fuels, cosmetics and others (Ye et al, 2016). In yeast, ERG12 encodes the mevalonate kinase, responsible for phosphorylation of mevalonate, whereas ERG20 encodes farnesyl pyrophosphate synthase, which catalyzes the formation of both farnesyl pyrophosphate (FPP) and geranyl pyrophosphate (GPP), units for sterol and isoprenoid biosynthesis (Chambon et al, 1991;Oulmouden and Karst, 1990).…”

Section: Application Of Casper For Engineering Key Enzymes In Yeast Mmentioning

confidence: 99%

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Jakočiūnas

Pedersen

Lis

et al. 2018

Metabolic Engineering

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Section: Application Of Casper For Engineering Key Enzymes In Yeast Mmentioning

confidence: 99%

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

Jakočiūnas

Pedersen

Lis

et al. 2018

Metabolic Engineering

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“…Similarly, isoprene can be prepared by dehydration of 3‐methyl‐3‐buten‐1‐ol, which can be generated from biomass sugars with metabolically engineered E. coli . Alternatively, isoprene can be directly obtained through fermentation and easily removed from the fermentation broth due to its low boiling point . 1‐Pentene can be generated by dehydration of biosynthetic 1‐pentanol with base‐treated alumina, or by Fischer–Tropsch (FT) oligomerization of bio‐derived synthesis gas.…”

Section: Resultsmentioning

confidence: 99%

“…[13] Alternatively,i soprene can be directly obtained through fermentation and easily removed from the fermentation broth due to its low boiling point. [40] 1-Pentene can be generated by dehydration of biosynthetic 1pentanol [14] with base-treated alumina, [41] or by Fischer-Tropsch (FT) oligomerization of bio-derived synthesis gas. The selective homo-and cross-dimerization of these C 5 and C 6 olefins would result in C 10 -C 12 products with molecular weights and physical properties within the range expected for conventionalj et fuel (C 9 -C 14 hydrocarbons).…”

Section: Resultsmentioning

confidence: 99%

High‐Performance Jet Fuels Derived from Bio‐Based Alkenes by Iron‐Catalyzed [2+2] Cycloaddition

2019

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A series of high‐performance cycloparaffinic fuels have been generated by [2+2] cycloaddition of the bio‐derived alkenes 1‐hexene, isoprene, and 1‐pentene, catalyzed by a low‐valent iron pyridine(diimine) complex [(MePDI)Fe(N2)2(μ‐N2)] [MePDI=N,N′‐(2,6‐pyridinediyldiethylidyne)bis(2,6‐dimethylbenzenamine)]. Reactions with 1‐pentene and 1‐hexene resulted in 85 % selectivity to 1,2‐cyclobutanes, and 12 % selectivity to acyclic alkenes generated by β‐hydride elimination. Self‐dimerization of isoprene was sluggish and generated heavier oligomer products, but cross‐dimerization of isoprene with 1‐hexene afforded primarily a 1,3‐cyclobutane product, along with isomers of acyclic C11 mixed dimers. Hydrocarbon mixtures were hydrogenated and fractionally distilled to yield finished fuel mixtures in overall yields of 83–93 % at the multigram scale. The fuels exhibited densities ranging between 0.767 and 0.783 g mL−1, and net heats of combustion (NHOC) of up to 120.6 kBtu gal−1 (43.8 MJ kg−1). These values are higher than conventional synthetic paraffinic kerosenes owing to the higher density and ring strain afforded by the cyclobutane rings. The fuel mixtures also exhibited extremely low viscosities ranging from 2.38 to 4.78 mm2 s−1 at −20 °C, due in part to the presence of the acyclic dimers. The excellent fuel properties of the product mixtures, selectivity for dimer products, high yields, and the ability to use simple bio‐derived alkenes as substrates, make the [Fe]‐catalyzed [2+2] cycloaddition of unactivated alkenes a compelling route to the synthesis of sustainable high‐performance fuels.

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“…Additionally, the price of isoprene has been greatly affected by fluctuating oil prices (Kim et al ). Therefore, microbial isoprene production from sustainable carbon sources has attracted interest as a potential alternative (Ye et al ).…”

Section: Introductionmentioning

confidence: 99%

Application of inorganic phosphate limitation to efficient isoprene production in Pantoea ananatis

et al. 2019

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Aims Establishment of an efficient isoprene fermentation process by adopting inorganic phosphate limitation as the trigger to direct metabolic flux to the isoprene synthetic pathway. Methods and Results We constructed isoprene‐producing strains of Pantoea ananatis (a member of the Enterobacteriaceae family) by integrating a heterologous mevalonate pathway and a metabolic switch that senses external inorganic phosphate (Pi) levels. This metabolic switch enabled dual‐phase isoprene production, where the initial cell growth phase under Pi‐saturating conditions was uncoupled from the subsequent isoprene production phase under Pi‐limiting conditions. In fed‐batch fermentation using our best strain (SWITCH‐PphoC/pIspSM) in a 1‐l bioreactor, isoprene concentration in the off‐gas was maintained between 300 and 460 ppm during the production phase and at 20 ppm during the cell growth phase, respectively. The strain SWITCH‐PphoC/pIspSM produced totally 2·5 g l−1 of isoprene from glucose with a 1·8% volumetric yield in 48 h. Conclusions This proof‐of‐concept study demonstrated that our Pi‐dependent dual‐phase production system using a P. ananatis strain as a producer has potential for industrial‐scale isoprene fermentation. Significance and Impact of the Study This Pi‐dependent dual‐phase fermentation process could be an attractive and economically viable option for the production of various commercially valuable isoprenoids.

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Engineering microbes for isoprene production

Cited by 81 publications

References 109 publications

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9

High‐Performance Jet Fuels Derived from Bio‐Based Alkenes by Iron‐Catalyzed [2+2] Cycloaddition

Application of inorganic phosphate limitation to efficient isoprene production in Pantoea ananatis

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