2019
DOI: 10.1111/jam.14521
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Application of inorganic phosphate limitation to efficient isoprene production in Pantoea ananatis

Abstract: Aims Establishment of an efficient isoprene fermentation process by adopting inorganic phosphate limitation as the trigger to direct metabolic flux to the isoprene synthetic pathway. Methods and Results We constructed isoprene‐producing strains of Pantoea ananatis (a member of the Enterobacteriaceae family) by integrating a heterologous mevalonate pathway and a metabolic switch that senses external inorganic phosphate (Pi) levels. This metabolic switch enabled dual‐phase isoprene production, where the initial … Show more

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Cited by 10 publications
(14 citation statements)
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“…1). This strain also has a metabolic switch to allow redirection of carbon flux to (S)-linalool via the MVA pathway by sensing P i -starvation with the PhoB/PhoR two-component system [23]. As a result of test-tube cultivation under P i -starved conditions using a biphasic fermentation system, SWITCH-PphoC Δgcd/ pAaLINS-ispA* produced 3.4 ± 0.2 g/L of (S)-linalool from 60 ± 0.0 g/L of glucose in 48 h (Table 1), which was greater than the reported linalool titers from yeasts [12,13,29,30] and bacteria [15,31].…”
Section: Search For Putative Rate-limiting Reaction To Increase (S)-lmentioning
confidence: 99%
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“…1). This strain also has a metabolic switch to allow redirection of carbon flux to (S)-linalool via the MVA pathway by sensing P i -starvation with the PhoB/PhoR two-component system [23]. As a result of test-tube cultivation under P i -starved conditions using a biphasic fermentation system, SWITCH-PphoC Δgcd/ pAaLINS-ispA* produced 3.4 ± 0.2 g/L of (S)-linalool from 60 ± 0.0 g/L of glucose in 48 h (Table 1), which was greater than the reported linalool titers from yeasts [12,13,29,30] and bacteria [15,31].…”
Section: Search For Putative Rate-limiting Reaction To Increase (S)-lmentioning
confidence: 99%
“…An external P i -dependent dual-phase fed-batch fermentation process was previously established for isoprene production using the SWITCH-PphoC strain [23]. The P i -starvation-inducible metabolic switch enables cells to grow efficiently under the P i -saturated phase and efficiently produce (S)-linalool under the subsequent P i -starved phase.…”
Section: Fed-batch Fermentation With Ip04/ Pblaaalins-ispa* Strainmentioning
confidence: 99%
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