Engineering
            <i>Pseudomonas putida</i>
            To Produce Rhamnolipid Biosurfactants for Promoting Phenanthrene Biodegradation by a Two-Species Microbial Consortium

Qin, Ruolin; Xu, Tao; Jia, Xiaoqiang

doi:10.1128/spectrum.00910-22

Cited by 10 publications

(4 citation statements)

References 46 publications

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“…These aromatic compounds are not only difficult to degrade, but their hydrophobicity decreases their bioavailability. Previous studies have shown that rhamnolipids can enhance the solubilization and degradation of PAHs (37,50,51). We hypothesized that RLMEs would likely exhibit increased ability to solubilize PAHs compared to typical rhamnolipids due to their methyl ester functionality.…”

Section: Ecological Implications For Biosurfactant Production Within ...mentioning

confidence: 97%

“…Rhamnolipid 1, first discovered from Pseudomonas aeruginosa (35), is known to exhibit antimicrobial properties and promote bacterial swarming motility (12)(13)(14)(15)(16)(17). Rhamnolipids have also been identified as potential mediators for uptake of hydrophobic substrates by P. aeruginosa and consortia (22,(36)(37)(38). As a result of carboxyl methylation, the typically anionic lipid region of rhamnolipids is neutralized in RLMEs, rendering this region even more lipophilic.…”

Section: Biosynthesis Of Rlmes Involves An Integral Membrane Methyltr...mentioning

confidence: 99%

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Surface-active antibiotic production is a multifunctional adaptation for postfire microbes

Liu,

Du,

Koupaei

et al. 2023

Preprint

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Wildfires affect soils in multiple ways, leading to numerous challenges for colonizing microbes. While it is thought that fire-adapted microbes lie at the forefront of postfire ecosystem recovery, the specific strategies that these microbes use to thrive in burned soils remain largely unknown. Through bioactivity screening of bacterial isolates from burned soils, we discovered that several Paraburkholderia spp. isolates produced a set of unusual rhamnolipid surfactants with a natural methyl ester modification. These rhamnolipid methyl esters (RLMEs) exhibited enhanced antimicrobial activity against other postfire microbial isolates, including pyrophilous Pyronema fungi and Amycolatopsis bacteria, compared to the typical rhamnolipids made by organisms such as Pseudomonas spp. RLMEs also showed enhanced surfactant properties and facilitated bacterial motility on agar surfaces. In vitro assays further demonstrated that RLMEs improved aqueous solubilization of polycyclic aromatic hydrocarbons, which are potential carbon sources found in char. Identification of the rhamnolipid biosynthesis genes in the postfire isolate, Paraburkholderia caledonica str. F3, led to the discovery of rhlM, whose gene product is responsible for the unique methylation of rhamnolipid substrates. RhlM is the first characterized bacterial representative of a large class of integral membrane methyltransferases that are widespread in bacteria. These results indicate multiple roles for RLMEs in the postfire lifestyle of Paraburkholderia isolates, including enhanced dispersal, solubilization of potential nutrients, and inhibition of competitors. Our findings shed new light on the chemical adaptations that bacteria employ in order to navigate, grow, and outcompete other soil community members in postfire environments.

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Section: Ecological Implications For Biosurfactant Production Within ...mentioning

confidence: 97%

Section: Biosynthesis Of Rlmes Involves An Integral Membrane Methyltr...mentioning

confidence: 99%

Surface-active antibiotic production is a multifunctional adaptation for postfire microbes

Liu,

Du,

Koupaei

et al. 2023

Preprint

View full text Add to dashboard Cite

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“…Due to PAHs with low bioavailability, a surfactant-enhanced bioremediation method was developed, and the interface tension of oil–water was effectively reduced, and ultimately improved the restoration potential of artificial MMS for the contaminated site (Martinez-Toledo et al, 2022 ). During biodegradation by a two-species microbial consortium, a biosurfactant-secreting bacteria ( Pseudomonas aeruginosa ) was introduced into artificial MMS, and the biodegradation rates of PHE were significantly increased from 61.15% to 73.86% (Qin et al, 2022 ). The strong ability of cell proliferation and PAH degradation was shown by single bacteria (Llasera et al, 2021 ), but the above strain usually lacks a comprehensive metabolic pathway and enzyme system for hydrocarbons.…”

Section: Artificial Mms Construction Strategymentioning

confidence: 99%

Artificial mixed microbial system for polycyclic aromatic hydrocarbons degradation

Cui¹,

He²,

Ntakirutimana³

et al. 2023

Front. Microbiol.

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Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants with major risks to human health. Biological degradation is environmentally friendly and the most appealing remediation method for a wide range of persistent pollutants. Meanwhile, due to the large microbial strain collection and multiple metabolic pathways, PAH degradation via an artificial mixed microbial system (MMS) has emerged and is regarded as a promising bioremediation approach. The artificial MMS construction by simplifying the community structure, clarifying the labor division, and streamlining the metabolic flux has shown tremendous efficiency. This review describes the construction principles, influencing factors, and enhancement strategies of artificial MMS for PAH degradation. In addition, we identify the challenges and future opportunities for the development of MMS toward new or upgraded high-performance applications.

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“… Pseudomonas fluorescens AH-40 achieved 97 % degradation of 150 mg·L −1 phenanthrene within 15 days, which was faster than previously reported pure cultures [14]. Pseudomonas putida produces rhamnolipid biosurfactants, thereby promoting phenanthrene biodegradation [15]. Notably, most strains could only degrade low molecular weight PAHs (such as fluoranthene and phenanthrene) at low rates [16].…”

Section: Introductionmentioning

confidence: 97%

Pseudomonas benzopyrenica sp. nov., isolated from soil, exhibiting high-efficiency degradation of benzo(a)pyrene

Dong,

Rao,

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, yellow-pigmented, aerobic and rod-shaped bacterium, designated as strain BaP3T, was isolated from the soil. Strain BaP3T grew at 16–37℃ (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). Additionally, strain BaP3T could tolerate NaCl concentrations in the range 0–6 % (optimum, 1%). Moreover, strain BaP3T was motile by flagella. The phylogenetic analysis of 16S rRNA sequences showed that strain BaP3T belonged to the genus Pseudomonas , and the sequence was most closely related to Pseudomonas oryzihabitans CGMCC 1.3392T and Pseudomonas psychrotolerans DSM 15758T, with 99.66 % sequence similarity. Pseudomonas rhizoryzae RY24T was the next closely related species, exhibiting 99.38 % 16S rRNA gene sequence similarity. The DNA–DNA hybridization and average nucleotide identity values between strain BaP3T and its closely related types were below 50 and 92 %, respectively. Both results were below the cut-off for species distinction. The genomic DNA G+C content of strain BaP3T was 65.30 mol%. The predominant quinone in strain BaP3T was identified as ubiquinone Q-9. The major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0. These results indicated that strain BaP3T represents a novel species in the genus Pseudomonas . The type strain is BaP3T (CCTCC AB 2022379T=JCM 35914T), for which the name Pseudomonas benzopyrenica sp. nov. is proposed.

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Engineering Pseudomonas putida To Produce Rhamnolipid Biosurfactants for Promoting Phenanthrene Biodegradation by a Two-Species Microbial Consortium

Abstract: Polycyclic aromatic hydrocarbons (PAHs), which are recalcitrant, carcinogenic, and tend to bioaccumulate, are widespread and persistent environmental pollutants. Based on these characteristics, the U.S.

Cited by 10 publications

References 46 publications

Surface-active antibiotic production is a multifunctional adaptation for postfire microbes

Surface-active antibiotic production is a multifunctional adaptation for postfire microbes

Artificial mixed microbial system for polycyclic aromatic hydrocarbons degradation

Pseudomonas benzopyrenica sp. nov., isolated from soil, exhibiting high-efficiency degradation of benzo(a)pyrene

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