Pseudomonas benzopyrenica sp. nov., isolated from soil, exhibiting high-efficiency degradation of benzo(a)pyrene

Abstract

A Gram-negative, yellow-pigmented, aerobic and rod-shaped bacterium, designated as strain BaP3T, was isolated from the soil. Strain BaP3T grew at 16–37℃ (optimum, 30 °C) and pH 6.0–8.0 (optimum, pH 7.0). Additionally, strain BaP3T could tolerate NaCl concentrations in the range 0–6 % (optimum, 1%). Moreover, strain BaP3T was motile by flagella. The phylogenetic analysis of 16S rRNA sequences showed that strain BaP3T belonged to the genus Pseudomonas , and the sequence was most closely related to Pseudomonas oryzihabitans CGMCC 1.3392T and Pseudomonas psychrotolerans DSM 15758T, with 99.66 % sequence similarity. Pseudomonas rhizoryzae RY24T was the next closely related species, exhibiting 99.38 % 16S rRNA gene sequence similarity. The DNA–DNA hybridization and average nucleotide identity values between strain BaP3T and its closely related types were below 50 and 92 %, respectively. Both results were below the cut-off for species distinction. The genomic DNA G+C content of strain BaP3T was 65.30 mol%. The predominant quinone in strain BaP3T was identified as ubiquinone Q-9. The major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0. These results indicated that strain BaP3T represents a novel species in the genus Pseudomonas . The type strain is BaP3T (CCTCC AB 2022379T=JCM 35914T), for which the name Pseudomonas benzopyrenica sp. nov. is proposed.

Whole-genome sequence of Pseudomonas benzopyrenica MLY92: isolation from diseased leaves of tobacco in China

“…Pseudomonas benzopyrenica belongs to the family Pseudomonadaceae in the phylum Bacterial , and has yellow, round, flattened colonies with smooth edges. The type strain BaP3 was isolated from soil samples in Hunan Province, China ( 1 ), and has been shown to effectively degrade benzo(a)pyrene ( 2 ). The strain described in this study was isolated from diseased tobacco leaves collected in Guizhou Province, China (25°02′24″ N, 104°54′00″E).…”

Phenotypic and Genomic Characterization of Pseudomonas wuhanensis sp. nov., a Novel Species with Promising Features as a Potential Plant Growth-Promoting and Biocontrol Agent

“…The name Pseudomonas was first proposed by Migula at the end of the 19th century [1]. The genus Pseudomonas includes a diverse microbial population with members that are derived from a wide range of environments, including water, air, soil, plants, and clinical materials [2][3][4][5][6][7][8][9][10]. According to the List of Prokaryotic Names with Standing in Nomenclature (LPSN; https://lpsn.dsmz.de (accessed on 30 December 2023)), Pseudomonas was the second-largest species, and more than 300 species had been validly published with correct names at the time of writing.…”

“…Members of the genus Pseudomonas are Gram-negative, aerobic, non-spore-forming, and rod-shaped, with one or several polar flagella [4,5,11]. Pseudomonas is known for its metabolic versatility and capacity to produce abundant secondary metabolites [6,12]. They can utilize various chemical compounds as sources of carbon, nitrogen, or phosphorus, making them interesting microorganisms for processes such as bioremediation and biotransformation [2,13].…”

Insight into the High-Efficiency Benzo(a)pyrene Degradation Ability of Pseudomonas benzopyrenica BaP3 and Its Application in the Complete Bioremediation of Benzo(a)pyrene

“…Pseudomonas benzopyrenica BaP3 is a highly efficient benzo(a)pyrene-degrading strain that was previously isolated by our group [29]. Compared with existing strains [12,22,23,30], strain BaP3 has a higher degradation rate and capacity; it can degrade benzo(a)pyrene by approximately 50% within 6 days.…”