Engineering human neo-tendon tissue in vitro with human dermal fibroblasts under static mechanical strain

Deng, Dan; Liu, Wei; Xu, Feng; Yang, Yang; Zhou, Guangdong; Zhang, Wen Jie; Cui, Lei; Cao, Yilin

doi:10.1016/j.biomaterials.2009.08.054

Cited by 123 publications

(111 citation statements)

References 24 publications

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“…Porcine dermal fibroblasts and TCs loaded on PLGA electro-spun fibres have been shown to promote tenogenic function in vitro and improved healing, as evidenced by improved gross morphology, histological analysis and biomechanical properties, in vivo [413][414][415][416]. In conjugation with BMSCs, electro-spun PLGA scaffolds have demonstrated suppression of lymphocytes in vitro and improved biomechanical properties and acceptable integration into the native tendon tissue [417].…”

Section: Bottom-up Approached For Tendon Repair Based On Synthetic Inmentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

176

188

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Section: Bottom-up Approached For Tendon Repair Based On Synthetic Inmentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

176

188

View full text Add to dashboard Cite

“…39,40 As shown in Figure 6, TEM examination revealed that the collagen fibrils remained immature (the diameter was small and the arrangement was random), mostly because the scaffold needs a longer culture time in vivo and more cyclic mechanical training. 40,41 The mechanical strength of the scaffold plays an important role in reconstructing abdominal wall defects, which can be used to predict the success or failure of the surgery. The mechanical strength provided by the scaffold, can be divided into two parts: the collagen fibrils of the SIS before degradation and the neo-tissue after implantation.…”

mentioning

confidence: 99%

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Song

Peng

Liu

et al. 2013

Tissue Engineering Part A

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The repair of abdominal wall defects following surgery remains a difficult challenge. Although multiple methods have been described to restore the integrity of the abdominal wall, there is no clear consensus on the ideal material for reconstruction. This study explored the feasibility of in vivo reconstruction of a rat model of an abdominal wall defect with a composite scaffold of tenocytes and porcine small intestinal submucosa (SIS). In the current study, we created a 2 · 1.5 cm abdominal wall defect in the anterolateral abdominal wall of SpragueDawley rats, which were assigned into three groups: the cell-SIS construct group, the cell-free SIS scaffold group, and the abdominal wall defect group. Tenocytes were obtained from the tendons of rat limbs. After isolation and expansion, cells (2 · 10 7 /mL) were seeded onto the three-layer SIS scaffolds and cultured in vitro for 5 days. Cell-SIS constructs or cell-free constructs were implanted to repair the abdominal wall defects. The results showed that the tenocytes could grow on the SIS scaffold and secreted corresponding matrices. In addition, both scaffolds could repair the abdominal wall defects with no hernia recurrence. In comparison to the cell-free SIS scaffold, the composite scaffold exhibited increased vascular regeneration and mechanical strength. Furthermore, following increased time in vivo, the mechanical strength of the composite scaffold became stronger. The results indicate that the composite scaffold can provide increased mechanical strength that may be suitable for repairing abdominal wall defects.

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“…2 Unfortunately, the clinical application of ESCs is restricted due to their limited availability (depending on the policy of different countries) and the complexity of cell manipulation required. Dermal fibroblasts have also been shown to form tendon tissue, [30][31][32] although further research has suggested that the healing process using skin-derived fibroblasts is suppressed with a lack of tenocyte markers and histopathologic correlations. 33,34 Being native cell sources, tenocytes and in situ fibroblasts are perhaps the most ideal cell sources for engineered tendon and ligament tissue, respectively.…”

Section: Introductionmentioning

confidence: 99%

Bioreactor Design for Tendon/Ligament Engineering

Wang

Gardiner

Lin

et al. 2013

Tissue Engineering Part B: Reviews

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Tendon and ligament injury is a worldwide health problem, but the treatment options remain limited. Tendon and ligament engineering might provide an alternative tissue source for the surgical replacement of injured tendon. A bioreactor provides a controllable environment enabling the systematic study of specific biological, biochemical, and biomechanical requirements to design and manufacture engineered tendon/ligament tissue. Furthermore, the tendon/ligament bioreactor system can provide a suitable culture environment, which mimics the dynamics of the in vivo environment for tendon/ligament maturation. For clinical settings, bioreactors also have the advantages of less-contamination risk, high reproducibility of cell propagation by minimizing manual operation, and a consistent end product. In this review, we identify the key components, design preferences, and criteria that are required for the development of an ideal bioreactor for engineering tendons and ligaments.

show abstract

Engineering human neo-tendon tissue in vitro with human dermal fibroblasts under static mechanical strain

Cited by 123 publications

References 24 publications

The past, present and future in scaffold-based tendon treatments

The past, present and future in scaffold-based tendon treatments

Reconstruction of Abdominal Wall Musculofascial Defects with Small Intestinal Submucosa Scaffolds Seeded with Tenocytes in Rats

Bioreactor Design for Tendon/Ligament Engineering

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