Engineering Functional Cartilage Grafts

Tan, Andrea R.; Hung, Clark T.

doi:10.1007/978-1-61779-322-6_13

Cited by 3 publications

(1 citation statement)

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“…Computational modeling may be used to guide alterations to the puzzle shape for improved growth and junctional integration (Nims et al, 2015). Integration of implanted engineered grafts with the surrounding native tissue remains a challenge in the field of regenerative medicine (Tan and Hung, 2011). In addition to attaching individual subunits, the aforementioned biocompatible tissue adhesives and osteochondral bases may help to secure grafts in the defect site.…”

Section: Discussionmentioning

confidence: 99%

A puzzle assembly strategy for fabrication of large engineered cartilage tissue constructs

Nover

Jones

et al. 2016

Journal of Biomechanics

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Engineering of large articular cartilage tissue constructs remains a challenge as tissue growth is limited by nutrient diffusion. Here, a novel strategy is investigated, generating large constructs through the assembly of individually cultured, interlocking, smaller puzzle-shaped subunits. These constructs can be engineered consistently with more desirable mechanical and biochemical properties than larger constructs (~4-fold greater Young's modulus). A failure testing technique was developed to evaluate the physiologic functionality of constructs, which were cultured as individual subunits for 28 days, then assembled and cultured for an additional 21-35 days. Assembled puzzle constructs withstood large deformations (40-50% compressive strain) prior to failure. Their ability to withstand physiologic loads may be enhanced by increases in subunit strength and assembled culture time. A nude mouse model was utilized to show biocompatibility and fusion of assembled puzzle pieces in vivo. Overall, the technique offers a novel, effective approach to scaling up engineered tissues and may be combined with other techniques and/or applied to the engineering of other tissues. Future studies will aim to optimize this system in an effort to engineer and integrate robust subunits to fill large defects.

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Section: Discussionmentioning

confidence: 99%