Engineering elastic bioactive composite hydrogels for promoting osteogenic differentiation of embryonic mesenchymal stem cells

Wang, Min; Guo, Yi; Deng, Zexing; Xu, Peng

doi:10.3389/fbioe.2022.1022153

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…To this aim, hydrogels have been recently used to construct templates guiding bone regeneration by promoting the differentiation of bone cells − Although their stiffness is insufficient for obtaining mineralized and “ready-to-use” bone tissue, they might provide an ideal, easily tunable platform for osteogenic differentiation and osteoblasts’ preosseus matrix secretion , Stiffness is a critical parameter in hydrogel-based BTE applications. In fact, it can dramatically influence cell behavior and tissue remodeling.…”

Section: Introductionmentioning

confidence: 99%

Tuning Physical Properties of GelMA Hydrogels through Microarchitecture for Engineering Osteoid Tissue

Walejewska,

Melchels,

Paradiso

et al. 2023

Biomacromolecules

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Gelatin methacryloyl (GelMA) hydrogels have gained significant attention due to their biocompatibility and tunable properties. Here, a new approach to engineer GelMAbased matrices to mimic the osteoid matrix is provided. Two crosslinking methods were employed to mimic the tissue stiffness: standard cross-linking (SC) based on visible light exposure (VL) and dual cross-linking (DC) involving physical gelation, followed by VL. It was demonstrated that by reducing the GelMA concentration from 10% (G10) to 5% (G5), the dual-cross-linked G5 achieved a compressive modulus of ∼17 kPa and showed the ability to support bone formation, as evidenced by alkaline phosphatase detection over 3 weeks of incubation in osteogenic medium. Moreover, incorporating poly(ethylene) oxide (PEO) into the G5 and G10 samples was found to hinder the fabrication of highly porous hydrogels, leading to compromised cell survival and reduced osteogenic differentiation, as a consequence of incomplete PEO removal.

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Section: Introductionmentioning

confidence: 99%

Tuning Physical Properties of GelMA Hydrogels through Microarchitecture for Engineering Osteoid Tissue

Walejewska,

Melchels,

Paradiso

et al. 2023

Biomacromolecules

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Dynamic and self-biodegradable polysaccharide hydrogel stores embryonic stem cell construct under ambient condition

Yang

Wei

Gao

et al. 2023

Front. Bioeng. Biotechnol.

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The proper microenvironment is critical for the storage and transportation of embryonic stem cells (ESCs). To mimic a dynamic 3D microenvironment as it exists in vivo and consider “off-the-shelf” availability reaching the destination, we proposed an alternative approach that allows for facile storage and transportation of stem cells in the form of ESCs-dynamic hydrogel construct (CDHC) under ambient conditions. To form CDHC, mouse embryonic stem cells (mESCs) were in-situ encapsulated within a polysaccharide-based dynamic and self-biodegradable hydrogel. After storing CDHC in a sterile and hermetic environment for 3 days and then transferring to a sealed vessel with fresh medium for another 3 days, the large and compact colonies retained a 90% survival rate and pluripotency. Furthermore, after transporting and arriving at the destination, the encapsulated stem cell could be automatically released from the self-biodegradable hydrogel. After continuous cultivation of 15 generations of retrieved cells, automatically released from the CDHC, the mESCs underwent 3D encapsulation, storage, transportation, release, and continuous long-term subculture; resumed colony forming capacity and pluripotency were revealed by stem cell markers both in protein and mRNA levels. We believe that the dynamic and self-biodegradable hydrogel provides a simple, cost-effective, and valuable tool for storing and transporting “ready-to-use” CDHC under ambient conditions, facilitating “off-the-shelf” availability and widespread applications.

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Engineering elastic bioactive composite hydrogels for promoting osteogenic differentiation of embryonic mesenchymal stem cells

Cited by 2 publications

References 47 publications

Tuning Physical Properties of GelMA Hydrogels through Microarchitecture for Engineering Osteoid Tissue

Tuning Physical Properties of GelMA Hydrogels through Microarchitecture for Engineering Osteoid Tissue

Dynamic and self-biodegradable polysaccharide hydrogel stores embryonic stem cell construct under ambient condition

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