Engineering Burkholderia sacchari to enhance poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] production from xylose and hexanoate

Oliveira-Filho, Edmar R.; Macedo, Matheus A. de; Lemos, Aline C.C.; Adams, Friederike; Merkel, Olivia M.; Taciro, Marilda Keico; Gómez, José; Silva, Luiziana Ferreira da

doi:10.1016/j.ijbiomac.2022.06.024

Cited by 12 publications

(2 citation statements)

References 71 publications

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“…(Yasin and Al-Mayaly 2021 ), Pseudomonas sp. (Kanavaki et al 2021 ), Aeromonas hydrophila (Szacherska et al 2022 ), Rhodopseudomonas palustris (Brown et al 2022 ), Burkholderia sacchari (Oliveira-Filho et al 2022 ), and Halomonas boliviensis (Arcila-Echavarría et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Baltacı,

Görmez

et al. 2024

Environ Sci Pollut Res

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Polyhydroxyalkanoates have attracted great interest as a suitable alternative to petrochemical based plastics due to their outstanding properties such as biodegradability and biocompatibility. However, the biggest problem in the production of microbial polyhydroxyalkanoates is low cost-effectiveness. In this study, polyhydroxyalkanoate production was carried out using waste substrates with local isolates. Culture conditions were optimized to increase the polyhydroxyalkanoate production potential. The produced polyhydroxyalkanoate was characterized by FTIR analyses, and its metabolic pathway was determined by real-time PCR. According to the results, the best polyhydroxyalkanoate producer bacteria was characterized as Pseudomonas neustonica NGB15. The optimal culture conditions were detected as 30 g/L banana peel powder, 25 °C temperature, pH 8, and 4-day incubation time. Under the optimized conditions, 3.34 g/L PHA production was achieved. As a result of FTIR analyses, major peaks were obtained at 1723, 1277, 1261, 1097, 1054, and 993 cm−1. These peaks represent that the type of produced polyhydroxyalkanoate was poly-β-hydroxybutyrate. According to gene expression profile of NGB15, it was determined that Pseudomonas neustonica NGB15 produces PHA using the de novo fatty acid synthesis metabolic pathway. In conclusion, poly-β-hydroxybutyrate production by Pseudomonas neustonica NGB15 using a low-cost fermentation medium has been shown to be biotechnologically promising.

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Section: Introductionmentioning

confidence: 99%

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Baltacı,

Görmez

et al. 2024

Environ Sci Pollut Res

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“…Because they do not contain toxic substances, they can be safely used in the human body without fear of adverse side effects. In addition, most types of PHAs can be synthesized from pure sugars such as xylose and hexanoates [17] and renewable resources such as corn, and many biowastes of plant and animal origin can be used as feed for the microbial production of PHAs, which makes it environmentally friendly [18][19][20][21][22][23]. However, PHAs have several disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Exploiting Polyhydroxyalkanoates for Biomedical Applications

2023

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Polyhydroxyalkanoates (PHA) are biodegradable plastic. Numerous bacteria produce PHAs under environmental stress conditions, such as excess carbon-rich organic matter and limitations of other nutritional elements such as potassium, magnesium, oxygen, phosphorus, and nitrogen. In addition to having physicochemical properties similar to fossil-fuel-based plastics, PHAs have unique features that make them ideal for medical devices, such as easy sterilization without damaging the material itself and easy dissolution following use. PHAs can replace traditional plastic materials used in the biomedical sector. PHAs can be used in a variety of biomedical applications, including medical devices, implants, drug delivery devices, wound dressings, artificial ligaments and tendons, and bone grafts. Unlike plastics, PHAs are not manufactured from petroleum products or fossil fuels and are, therefore, environment-friendly. In this review, a recent overview of applications of PHAs with special emphasis on biomedical sectors, including drug delivery, wound healing, tissue engineering, and biocontrols, are discussed.

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Exploitation of Biomass to the Integrated Production of Bioethanol and Poly(hydroxyalkanoate)s

Trapé,

López,

Villar

2023

Bioenerg. Res.

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Engineering Burkholderia sacchari to enhance poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] production from xylose and hexanoate

Cited by 12 publications

References 71 publications

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Exploiting Polyhydroxyalkanoates for Biomedical Applications

Exploitation of Biomass to the Integrated Production of Bioethanol and Poly(hydroxyalkanoate)s

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