Engineering anisotropic cardiac monolayers on microelectrode arrays for non-invasive analyses of electrophysiological properties

Alassaf, Ahmad; Tansık, Gülistan; Mayo, Vera; Wubker, Laura; Carbonero, Daniel; Agarwal, Ashutosh

doi:10.1039/c9an01339c

Cited by 20 publications

(16 citation statements)

References 59 publications

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“…In another work, a microelectrode array platform was fabricated for recording the beating rate and conductive velocity of cardiomyocytes. In this device, the cardiac monolayer beat in a synchronized fashion, and the conduction velocity was close to the physiological value [208]. Different methods can measure the beating of heart tissue.…”

Section: Implementation Of Physical Forcesmentioning

confidence: 75%

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Sharma

Dash

Govarthanan

et al. 2021

Cells

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Myocardium Infarction (MI) is one of the foremost cardiovascular diseases (CVDs) causing death worldwide, and its case numbers are expected to continuously increase in the coming years. Pharmacological interventions have not been at the forefront in ameliorating MI-related morbidity and mortality. Stem cell-based tissue engineering approaches have been extensively explored for their regenerative potential in the infarcted myocardium. Recent studies on microfluidic devices employing stem cells under laboratory set-up have revealed meticulous events pertaining to the pathophysiology of MI occurring at the infarcted site. This discovery also underpins the appropriate conditions in the niche for differentiating stem cells into mature cardiomyocyte-like cells and leads to engineering of the scaffold via mimicking of native cardiac physiological conditions. However, the mode of stem cell-loaded engineered scaffolds delivered to the site of infarction is still a challenging mission, and yet to be translated to the clinical setting. In this review, we have elucidated the various strategies developed using a hydrogel-based system both as encapsulated stem cells and as biocompatible patches loaded with cells and applied at the site of infarction.

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Section: Implementation Of Physical Forcesmentioning

confidence: 75%

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Sharma

Dash

Govarthanan

et al. 2021

Cells

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“…This arrhythmogenic behavior of the corrected CMs was replicated with CMs derived from the hESC H9 control line. MEA measurements on fibronectin coated glass substrate have been reported to cause more beating variation, compared to more ideal culture systems such as hydrogels 48 , that more closely resemble the in vivo extracellular environment. Since the ECM is assumed to be abnormal in MFS, it could be suggested that the MFS CMs receive less mechanical feedback signals from the environment, explaining the small amount of variation in beat rate 49 .…”

Section: Discussionmentioning

confidence: 99%

Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome

Aalders

Léger

Meeren

et al. 2020

Sci Rep

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Marfan syndrome (MFS) is a systemic disorder of connective tissue caused by pathogenic variants in the fibrillin-1 (FBN1) gene. Myocardial dysfunction has been demonstrated in MFS patients and mouse models, but little is known about the intrinsic effect on the cardiomyocytes (CMs). In this study, both induced pluripotent stem cells derived from a MFS-patient and the line with the corrected FBN1 mutation were differentiated to CMs. Several functional analyses are performed on this model to study MFS related cardiomyopathy. Atomic force microscopy revealed that MFS CMs are stiffer compared to corrected CMs. The contraction amplitude of MFS CMs is decreased compared to corrected CMs. Under normal culture conditions, MFS CMs show a lower beat-to-beat variability compared to corrected CMs using multi electrode array. Isoproterenol-induced stress or cyclic strain demonstrates lack of support from the matrix in MFS CMs. This study reports the first cardiac cell culture model for MFS, revealing abnormalities in the behavior of MFS CMs that are related to matrix defects. Based on these results, we postulate that impaired support from the extracellular environment plays a key role in the improper functioning of CMs in MFS.

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“…Disease modeling can be achieved by manipulating the mechanical properties of the biomaterials employed, including extracellular matrix hydrogels [128] or synthetic fibers, which impact on cell attachment, alignment and stiffness [129]. Moreover, cardiac cells can be integrated in microfluidic devices that recapitulate in vivo microenvironment complexity, including electrical stimulation, cyclic stretch, fluid flow and chemical gradients generation [129].…”

Section: Cultured Cell-based Approaches To Study Cardiac Intercellular Communicationmentioning

confidence: 99%

Intercellular Communication in the Heart: Therapeutic Opportunities for Cardiac Ischemia

Martins‐Marques

Hausenloy

Sluijter

et al. 2021

Trends in Molecular Medicine

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Engineering anisotropic cardiac monolayers on microelectrode arrays for non-invasive analyses of electrophysiological properties

Abstract: Engineering cardiac tissues with physiological architectural and mechanical properties on microelectrode arrays enables long term culture and non-invasive collection of electrophysiological readouts.

Cited by 20 publications

References 59 publications

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome

Intercellular Communication in the Heart: Therapeutic Opportunities for Cardiac Ischemia

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