Engineering a heterologous synthetic pathway in Escherichia coli for efficient production of biotin

Wei, Peipei; Zhu, Furong; Chen, Cunwu; Li, Guosi

doi:10.1007/s10529-021-03108-y

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…63 When developing the biotin biosynthesis (involving SAM-mediated methylations) in E. coli, Wei et al improved biotin production from ∼25 mg/L to 92.6 mg/L in shaking flask fermentation by feeding 0.2 g/L L-methionine and 1.2 g/L pimelic acid (a precursor of biotin). 15 As for the eukaryotic microorganisms, S. cerevisiae also possesses two L-methionine importing transporters, Mup1p and Mup3p, among which Mup1p is the primary and high affinity one. 64 However, these transporters suffer from the regulation of excess extracellular L-methionine at both transcription and post-translation (protein activity) levels.…”

Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%

“…When developing the biotin biosynthesis (involving SAM-mediated methylations) in E. coli, Wei et al improved biotin production from ∼25 mg/L to 92.6 mg/L in shaking flask fermentation by feeding 0.2 g/L l -methionine and 1.2 g/L pimelic acid (a precursor of biotin) . As for the eukaryotic microorganisms, S.…”

Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%

“…S -Adenosyl- l -methionine (SAM or AdoMet) is the primary methyl donor in methylation reactions in nature . The SAM-dependent natural products (SdNPs) have been widely used in pharmaceutical (such as pterostilbene, (2 S )-sakuranetin, eugenol, nocamycin, , gentamicin C, and ferulic acid), nutritional (such as vitamin B12, O -methyl anthocyanin, betaine, and biotin), cosmetic (such as methyl cinnamate and ergothioneine), flavor (such as vanillin and N -methylanthranilates), and energy (such as methyl esters) fields. The demand for SdNPs has increased dramatically in recent years. , Traditional approaches to SdNPs include chemical synthesis and crude extraction, however, suffer from low chiral selectivity and yield. , Motivated by the great demand, the past decades have seen substantial advances in developing microbial cell factory (MCF) to produce SdNPs (Table ), which are believed to be promising alternatives to the traditional methods .…”

Section: Introductionmentioning

confidence: 99%

“…S-Adenosyl-L-methionine (SAM or AdoMet) is the primary methyl donor in methylation reactions in nature. 4 The SAM-dependent natural products (SdNPs) have been widely used in pharmaceutical (such as pterostilbene, 5 (2S)sakuranetin, 6 eugenol, 7 nocamycin, 8,9 gentamicin C, 10 and ferulic acid 11 ), nutritional (such as vitamin B12, 12 O-methyl anthocyanin, 13 betaine, 14 and biotin 15 ), cosmetic (such as methyl cinnamate 16 and ergothioneine 17 ), flavor (such as vanillin 18 and N-methylanthranilates 19 ), and energy (such as methyl esters 20 ) fields. The demand for SdNPs has increased dramatically in recent years.…”

Section: Introductionmentioning

confidence: 99%

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Improving Microbial Cell Factory Performance by Engineering SAM Availability

Lv,

Chang,

Zhang

et al. 2024

J. Agric. Food Chem.

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Methylated natural products are widely spread in nature. S-Adenosyl-L-methionine (SAM) is the secondary abundant cofactor and the primary methyl donor, which confer natural products with structural and functional diversification. The increasing demand for SAM-dependent natural products (SdNPs) has motivated the development of microbial cell factories (MCFs) for sustainable and efficient SdNP production. Insufficient and unsustainable SAM availability hinders the improvement of SdNP MCF performance. From the perspective of developing MCF, this review summarized recent understanding of de novo SAM biosynthesis and its regulatory mechanism. SAM is just the methyl mediator but not the original methyl source. Effective and sustainable methyl source supply is critical for efficient SdNP production. We compared and discussed the innate and relatively less explored alternative methyl sources and identified the one involving cheap one-carbon compound as more promising. The SAM biosynthesis is synergistically regulated on multilevels and is tightly connected with ATP and NAD(P)H pools. We also covered the recent advancement of metabolic engineering in improving intracellular SAM availability and SdNP production. Dynamic regulation is a promising strategy to achieve accurate and dynamic fine-tuning of intracellular SAM pool size. Finally, we discussed the design and engineering constraints underlying construction of SAM-responsive genetic circuits and envisioned their future applications in developing SdNP MCFs.

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Section: Sam Biosynthetic Pathwaymentioning

confidence: 99%