Engineering 3D Aligned Nanofibers for Regulation of Cell Growth Behavior

Abstract

3D aligned electrospun fibers hold a promising potential in a wide range of biomedical areas, including biosensors, controlled drug release, tissue engineering, etc. Thus, a cost‐effective and easy way to scale‐up fabrication for 3D aligned nanofibers is highly desired. Herein, a novel yet facile preparation process of 3D aligned nanofibers (3D AFs) by an improved electrospinning technique is reported. The obtained 3D AFs show enhanced controllability on morphology and fiber density, and thus facilitate adhesion and growth of human mesenchymal stem cells within their 3D nanofiber microarchitectures, leading to an excellent in vitro biocompatibility. Moreover, the 3D AFs with aligned morphology can enhance the neuron activities and induce directional cell growth along the direction of nanofiber orientation, thereby providing an excellent cue for the anchorage and migration dependent neurons. Combined with controllable morphology and structure, it is anticipated that this finding can lead to great applications of electrospun fibers in nerve tissue engineering, diagnostics, and other biomedical fields.

A three‐dimensional carbon nanotube–nanofiber composite foam for selective adsorption of oils and organic liquids

“…8, which demonstrated that the sorption of 3D CNTs-NFs was higher compared with most of previous reported sorbent [35,[38][39][40][41][42][43]. Although the adsorption property of the 3D CNTs-NFs was still lower compared with the graphene/CNT foam [44], the production of 3D CNTs-NFs is simple and cost effective. More importantly, its fabrication can be scaled-up.…”

A three-dimensional hydroxyapatite/polyacrylonitrile composite scaffold designed for bone tissue engineering

“…BMSCs were then seeded into/onto each 3D HA/PAN composite scaffold and HA/PAN composite membrane at a density of 1.0 Â 10 4 cells per well according to previous method. 38,39 Aer 2 h cell attachment, 0.5 ml complete culture medium was added into each well. The resulting substrates/cell constructs were cultured in an incubator at 37 C, 95% relative humidity and 5% CO 2 partial pressure.…”

Preparation and Magnetic Properties of CoFe2O4 Oriented Fiber Arrays by Electrospinning

“…Electrospinning technology [ 18 , 19 ] has the advantages of simple preparation equipment, low spinning cost, and controllable process, and has become one of the main ways to effectively prepare nanofibers. By changing the receiving device of electrospinning, controlling the electric field, and including an additional magnetic field, we can obtain different forms of aligned nanofiber assemblies including oriented nanofiber membranes, oriented nanofiber bundles, and oriented nanofiber coated yarns, which will further expand the application field of directional nanofiber [ 20 , 21 , 22 , 23 , 24 , 25 ].…”