Engineered resistance against plant virus diseases

Nejidat, Ali; Clark, W. Gregg; Beachy, Roger N.

doi:10.1034/j.1399-3054.1990.800426.x

Cited by 4 publications

(2 citation statements)

References 24 publications

(33 reference statements)

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“…However, recently produced transgenic plants with enhanced resistance to fungi have been constructed (BROGLIE et al 1991). Engineering of virus resistant plants via coat proteins (VAN DEN ELZEN et al 1989;NEJIDAT et al 1990;TIMMERMAN 1991) and satellite RNA GALLITELLI et al 1991) was more successful.…”

Section: Genomic Librariesmentioning

confidence: 99%

Impact of molecular biology on forest pathology: A literature review

Hubbes

1993

European Journal of Forest Pathology

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Rapid developments in the field of molecular biology have produced a number of very powerful analytical tools by unifying techniques of genetics, biochemistry and cell biology. As reviewed in the present paper, these tools will become important and indispensable in forest pathology studies of the genetic machinery and regulatory mechanisms that control fungal products implicated in the complex tree-pathogen interactions. Understanding these interactions will enhance the development of efficient control methods of forest pathogens.

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Section: Genomic Librariesmentioning

confidence: 99%

Impact of molecular biology on forest pathology: A literature review

Hubbes

1993

European Journal of Forest Pathology

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“…Cucumis melo (cantaloupe, melon, or muskmelon) is subject to several viral diseases that severely limit yield and for which adequate levels of native resistance are not available. A solution to this problem may be to genetically engineer virus resistance into existing germplasm (Nejidat et al, 1990). Adaptation of this technology to commercial varieties and breeding lines of C. melo will require a dependable, high-frequency cell regeneration system.…”

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confidence: 99%

High-frequency Somatic Embryogenesis from Quiescent Seed Cotyledons of Cucumis melo Cultivars

Gray¹,

McColley²,

Compton³

1993

jashs

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A protocol for high-frequency somatic embryogenesis in Cucumis melo L. was developed using `Male Sterile A147 as a model cultivar. Basal halves of quiescent seed cotyledons were cultured on embryo induction (EI) medium containing concentration ranges of the auxin 2,4-D and the cytokinins BA, Bin, TDZ, or 2iP before transfer to embryo development (ED) medium. Medium with 2,4-D at 5 mg·liter-1 and TDZ at 0.1 mg·liter-1 was superior, with 49% of explants responding and an average of 3.3 somatic embryos per explant (6.8 somatic embryos per responding explant). More explants produced embryos when incubated on EI medium for 1 or 2 weeks (30% and 33%) than for 3 or 4 weeks or with no induction. However, 2 weeks was 2.9 times better than 1 week in terms of number of embryos per explant. One week of initial culture in darkness, followed by a 16 hour light/8 hour dark photoperiod, produced more responding explants (26%) than two or more weeks in darkness or no dark period at all; but 1 and 2 weeks of darkness resulted in a similar number of embryos per explant (2.1 and 2.8). Sucrose concentration in EI and ED media had a highly significant effect on embryo induction and development. EI medium with 3% sucrose resulted in more embryogenic explants than EI medium with 1.5% or 6% sucrose. However, treatments with 3% sucrose in EI medium and 3% or 6% sucrose in ED medium produced significantly more embryos per explant (8.5 and 11.9) than other treatments. Treatments did not affect embryo induction directly and regeneration per se but, instead, frequency and efficiency of somatic embryo development. The optimal treatments were tested with 51 other commercial varieties. All varieties underwent somatic embryogenesis, exhibiting a response of 5% to 100% explant response and 0.1-20.2 embryos per explant. Chemical names used: N-(phenylmethyl)-lH-purin-6-amine (benzyladenine or BA); N-(2-furanylmethyl)-lH-purin-6-amine (kinetin or BIN); N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron or TDZ); N-(3-methyl-2-butenyl)-lH-purin-6-amine (2iP); (2,4-dichlorophenoxy) acetic acid (2,4-D).

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