Engineered Gut Symbiotic Bacterium-Mediated RNAi for Effective Control of
            <i>Anopheles</i>
            Mosquito Larvae

Ding, Jinjin; Cui, Chunlai; Wang, Guandong; Ge, Wei; Bai, Liang; Li, Yifei; Sun, Peilu; Dong, Le; Liu, Zichen; Jianbing, Yun; Li, Fang; Li, Kai; Wang, Sibao

doi:10.1128/spectrum.01666-23

Cited by 4 publications

(1 citation statement)

References 43 publications

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“…RNAi has been described to be a promising technique for pest and mosquito control [12][13][14][15][16]. It is an important tool for identifying potential insecticidal targets that could be explored to develop insecticides or other products to limit the burden of mosquitoes on human health [17].…”

Section: Introductionmentioning

confidence: 99%

Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control

Adedeji,

Beder,

Damiani

et al. 2024

PLoS ONE

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Increasing reports of insecticide resistance continue to hamper the gains of vector control strategies in curbing malaria transmission. This makes identifying new insecticide targets or alternative vector control strategies necessary. CLassifier of Essentiality AcRoss EukaRyote (CLEARER), a leave-one-organism-out cross-validation machine learning classifier for essential genes, was used to predict essential genes in Anopheles gambiae and selected predicted genes experimentally validated. The CLEARER algorithm was trained on six model organisms: Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Saccharomyces cerevisiae and Schizosaccharomyces pombe, and employed to identify essential genes in An. gambiae. Of the 10,426 genes in An. gambiae, 1,946 genes (18.7%) were predicted to be Cellular Essential Genes (CEGs), 1716 (16.5%) to be Organism Essential Genes (OEGs), and 852 genes (8.2%) to be essential as both OEGs and CEGs. RNA interference (RNAi) was used to validate the top three highly expressed non-ribosomal predictions as probable vector control targets, by determining the effect of these genes on the survival of An. gambiae G3 mosquitoes. In addition, the effect of knockdown of arginase (AGAP008783) on Plasmodium berghei infection in mosquitoes was evaluated, an enzyme we computationally inferred earlier to be essential based on chokepoint analysis. Arginase and the top three genes, AGAP007406 (Elongation factor 1-alpha, Elf1), AGAP002076 (Heat shock 70kDa protein 1/8, HSP), AGAP009441 (Elongation factor 2, Elf2), had knockdown efficiencies of 91%, 75%, 63%, and 61%, respectively. While knockdown of HSP or Elf2 significantly reduced longevity of the mosquitoes (p<0.0001) compared to control groups, Elf1 or arginase knockdown had no effect on survival. However, arginase knockdown significantly reduced P. berghei oocytes counts in the midgut of mosquitoes when compared to LacZ-injected controls. The study reveals HSP and Elf2 as important contributors to mosquito survival and arginase as important for parasite development, hence placing them as possible targets for vector control.

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Section: Introductionmentioning

confidence: 99%

Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control

Adedeji,

Beder,

Damiani

et al. 2024

PLoS ONE

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Functions and regulations of insect gut bacteria

Han,

Akhtar,

Xia

2024

Pest Management Science

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The insect gut is a complicated ecosystem that inhabits a large number of symbiotic bacteria. As an important organ of the host insect, the symbiotic bacteria of the insect gut play very important roles in regulating physiological and metabolic processes. Recently, much progress has been made in the study of symbiotic bacteria in insect guts with the development of high‐throughput sequencing technology and molecular biology. This review summarizes the primary functions of symbiotic bacteria in insect guts, such as enhancing insecticide resistance, facilitating food digestion, promoting detoxification, and regulating mating behavior and egg hatching. It also addresses some possible pathways of gut bacteria symbiont regulation governed by external habitats, physiological conditions and immunity of the host insect. This review provides solid foundations for further studies on novel theories, new technologies and practical applications of symbiotic bacteria in insect guts. © 2024 Society of Chemical Industry.

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Limitations in harnessing oral RNA interference as an antiviral strategy in Aedes aegypti

Romoli,

Henrion-Lacritick,

Blanc

et al. 2024

iScience

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Engineered Gut Symbiotic Bacterium-Mediated RNAi for Effective Control of Anopheles Mosquito Larvae

Abstract: Mosquitoes are vectors for various diseases, imposing a significant threat to public health globally. The recent invasion of A. stephensi in Africa has made malaria eradication more challenging due to its outdoor biting behavior and widespread resistance to insecticides.

Cited by 4 publications

References 43 publications

Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control

Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control

Functions and regulations of insect gut bacteria

Limitations in harnessing oral RNA interference as an antiviral strategy in Aedes aegypti

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