Energy Transfer-Based Multiplex Analysis Using Quantum Dots

Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine

“…QDs are capable of high quantum yield, improved sensitivity, and high photostability. They have size-tunable emission wavelengths, and have paved the way for numerous studies including imaging, sensing and targeting biomolecules. , Thus, QDs are now rapidly replacing traditional fluorophores in almost all fluorescence-based applications . However, it is only recently that QDs have been applied to CRET systems.…”

“…Ren et al were the first to demonstrate efficient CRET between luminol as the energy donor and CdTe core QDs as the acceptor, based on an immuno-interaction of BSA-QDs and anti-BSA-HRP in the luminol-H 2 O 2 chemiluminescence reaction. Since the work of Ren et al, the QD-based CRET has now been applied to the detection of small molecules (e.g., H 2 O 2 , adenosine triphosphate and glucose), DNA, and proteins. , In these applications, core or core/shell QDs were used and they were usually functionalized with macromolecules, such as proteins, antibodies, and an aptamer . A key approach to improve the detection sensitivity of the CRET-based bioassay is to enhance the CRET efficiency.…”

“…3,13 Thus, QDs are now rapidly replacing traditional fluorophores in almost all fluorescence-based applications. 14 However, it is only recently that QDs have been applied to CRET systems. Ren et al 15 were the first to demonstrate efficient CRET between luminol as the energy donor and CdTe core QDs as the acceptor, based on an immuno-interaction of BSA-QDs and anti-BSA-HRP in the luminol-H 2 O 2 chemiluminescence reaction.…”

“…Since the work of Ren et al, 15 the QD-based CRET has now been applied to the detection of small molecules (e.g., H 2 O 2 , adenosine triphosphate and glucose), DNA, and proteins. 14,16 In these applications, core or core/shell QDs were used and they were usually functionalized with macromolecules, such as proteins, antibodies, and an aptamer. 17 A key approach to improve the detection sensitivity of the CRET-based bioassay is to enhance the CRET efficiency.…”