Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine

Ma, Mingfang; Wen, Kai; Beier, Ross C.; Eremin, Sergei A.; Li, Chenglong; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui

doi:10.1021/acsami.6b04171

Cited by 44 publications

(12 citation statements)

References 30 publications

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“…GNPs and polymer-modified core/multishell QDs were previously prepared by our group. , Goat anti-mouse immunoglobulin G (IgG) was from Jackson Immuno-Research Laboratories (West Grove, PA). Bovine serum albumin (BSA) was from Sigma-Aldrich.…”

Section: Methodsmentioning

confidence: 99%

Multiplex Lateral Flow Immunoassays Based on Amorphous Carbon Nanoparticles for Detecting Three Fusarium Mycotoxins in Maize

Zhang

Wen

et al. 2017

J. Agric. Food Chem.

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124

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The detecting labels used for lateral flow immunoassays (LFAs) have been traditionally gold nanoparticles (GNPs) and, more recently, luminescent nanoparticles, such as quantum dots (QDs). However, these labels have low sensitivity and are costly, in particular, for trace detection of mycotoxins in cereals. Here, we provided a simple preparation procedure for amorphous carbon nanoparticles (ACNPs) and described multiplex LFAs employing ACNPs as labels (ACNP-LFAs) for detecting three Fusarium mycotoxins. The analytical performance of ACNPs in LFA was compared to GNPs and QDs using the same immunoreagents, except for the labels, allowing for their analytical characteristics to be objectively compared. The visual limit of detection for ACNP-LFAs in buffer was 8-fold better than GNPs and 2-fold better than QDs. Under optimized conditions, the quantitative limit of detection of ACNP-LFAs in maize was as low as 20 μg/kg for deoxynivalenol, 13 μg/kg for T-2 toxin, and 1 μg/kg for zearalenone. These measurements were much lower than the action level of these mycotoxins in maize. The accuracy and precision of the ACNP-LFAs were evaluated by analysis of spiked and incurred maize samples with recoveries of 84.6-109% and coefficients of variation below 13%. The results of ACNP-LFAs using naturally incurred maize samples showed good agreement with results from high-performance liquid chromatography-tandem mass spectrometry, indicating that ACNPs were more sensitive labels than and a promising alternative to GNPs used in LFAs for detecting mycotoxins in cereals.

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Section: Methodsmentioning

confidence: 99%

Multiplex Lateral Flow Immunoassays Based on Amorphous Carbon Nanoparticles for Detecting Three Fusarium Mycotoxins in Maize

Zhang

Wen

et al. 2017

J. Agric. Food Chem.

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124

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“…More recently, more CRET platforms with traditional CL systems using luminophor donors and with metal‐based QDs as energy acceptors have been extensively reported . Suh and Park's group designed a reactive oxygen species (ROS)‐responsive hybrid nanoparticles (HNPs) composed of polyethylene glycol (PEG)‐modified QDs (PEG−QDs) that acted as energy acceptors and using a luminol derivative (L012)‐based CL system as the CRET donor.…”

Section: Semiconductor Quantum Dotsmentioning

confidence: 99%

Quantum dots‐based chemiluminescence probes: an overview

Song

Zhang

et al. 2019

Luminescence

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This mini-review describes the recent developments in quantum dots-based nanoprobes in liquid-phase chemiluminescence (CL) analysis. In the referenced reports, multiple quantum dots (QDs) were adopted as final emission species either after direct oxidation reactions (direct CL) or after chemiluminescence resonance energy transfer (indirect CL). This review does not include papers in which QDs have been used as enhancers, catalysts, carriers or quenchers in chemiluminescence systems. A brief overview on the CL mechanisms of various QDs-based nanoprobes and their analytical applications over the last decade is given, followed by comments on the future challenges and prospects in this field. KEYWORDS chemiluminescence (CL), nanoprobe, quantum dots (QDs)

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“…11,12 Although these methods are standardized, accurate and reliable, their wide applications are limited by high costs and complex sample preparations. 13 In addition, antibody-dependent methods including enzyme-linked immunosorbent assay (ELISA), 14 chemiluminescence, 15 time-resolved immunoassay, 16,17 lateral flow immunoassay 18 and electrochemical immunosensor 19 also face different challenges such as poor stabilities, high costs, and difficulties in modifying antibody structures. 20 Aptamers are single-stranded DNA or RNA that bind specifically to target molecules, and they are selected by an in vitro process called systematic evolution of ligands by exponential enrichment (SELEX).…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Label-Free Fluorescent Aptasensor Platform for Detection of Sulfamethazine

Wang¹,

Yan²,

Kou³

et al. 2021

IJN

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Purpose: Sulfamethazine (SMZ) exposed in the environment can enter the human body through the food chain and pose a serious threat to human health. Therefore, it is important to develop a rapid and sensitive method for detecting SMZ in environmental samples. In order to fastly and quantitatively detect SMZ in environmental samples, we developed a label-free fluorescent aptasensor based on specific aptamer (SMZ1S) and fluorescence resonance energy transfer (FRET) between gold nanoparticles (AuNPs) and rhodamine B (RhoB). Methods: In the absence of SMZ, SMZ1S was adsorbed on the surface of AuNPs, which led to dispersion of the AuNPs in high concentration saline solution, thus effectively quenching the fluorescence of RhoB. With the increase of the SMZ concentration, the specific binding of SMZ1S and SMZ led to the aggregation of AuNPs in the presence of NaCl, which reduced the quenching of RhoB fluorescence and increased the fluorescence intensity. The sensitivity and linearity curve of the label-free fluorescent aptasensor were determined with different concentrations of sulfamethazine standard solutions. The specificity of this fluorescent aptasensor was determined by replacing sulfamethazine with different antibiotics. In addition, the actual water and soil samples were spiked and recovered. Results: Under optimized conditions, the proposed fluorescent aptasensor demonstrated a good linear detection of SMZ in binding buffer from 1.25 ng mL −1 to 40 ng mL −1 and the limit of detection was 0.82 ng mL −1 . The spiked recoveries for SMZ were 94.4% to 108.8% with a relative standard deviation of 1.8-10.3% in water and soil samples, respectively. Conclusion:The label-free fluorescent aptasensor investigated in the current study is a promising tool to detect and quantify SMZ in water and soil samples.

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Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine

Cited by 44 publications

References 30 publications

Multiplex Lateral Flow Immunoassays Based on Amorphous Carbon Nanoparticles for Detecting Three Fusarium Mycotoxins in Maize

Multiplex Lateral Flow Immunoassays Based on Amorphous Carbon Nanoparticles for Detecting Three Fusarium Mycotoxins in Maize

Quantum dots‐based chemiluminescence probes: an overview

An Ultrasensitive Label-Free Fluorescent Aptasensor Platform for Detection of Sulfamethazine

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