Energy Pumping by Surface Collectors on Upconversion Nanoparticles for Extended Transfer and Efficient Self-Evaluable Photodynamic Therapy

Abstract

Although upconversion nanoparticle (UCNP) based luminescence resonance energy transfer (LRET) has attracted intensive attention, its application in sensing strategy for biomacromolecule detection is still limited due to the low energy transfer efficiency. Here we develop an energy pumping strategy to enhance the upconversion LRET efficiency of UCNP from 3.6% to 70.3%. Surface collector dye Cy3 is modified on UCNP surface to concentrate the scattered emission energy from interior UCNP to particle surface and further pump to surface extended acceptor. Using caspase-3 as a target, its recognition and cleavage of QSY7 labeled peptide that co-assembled on UCNP-Cy3 leads to the release of QSY7 for recovering Cy3 fluorescence. Owing to the

Nanoprobe-based molecular imaging for tumor stratification

“…201 Another UCNP-based self-evaluation nanoplatform for PDT was constructed by Zhang et al , and the nanoplatform consisted of QSY7-labeled DEVD peptide, photosensitizer pyropheophorbide-a (Ppa), fluorescent molecule Cy3, and UCNP. 202 The absorption and emission wavelengths of Cy3 match the UCL emission (540 nm) and QSY7 absorption, respectively.…”

Small‐Molecule Quenchers for Förster Resonance Energy Transfer: Structure, Mechanism, and Applications

“…Detect caspase-3 and survivin mRNA, [26] Uracil DNA glycosylases (Ung) [35] /MMP-3, [30] /MMPs [39] /MMP-3, [31] hypoxic tissues [41] Detect miR-21, [44] Zika-specific DNA in human blood serum, [45] the activity of anti-digoxigenin (Dig) antibodies, [46] intracellular target mRNA [47] /caspase-3 [48] IQ 4 587 520-706 56 000 -/--/-Eclipse 522 460-570 -/-FAM/aminoacridine Detect Guignardia citricarpa, [50] human immunodeficiency virus type 1 (HIV-1) in plasma [51] /single base pair mutations [52] TAMRA 544 470-560 85 000 FAM Detect AFB1 in beer, wine samples, [58] the mutation level of Kisten rat sarcoma (KRAS) viral gene [59] QSY7 560 500-600 92 000 Cy3/BODIPY/fluorescein (92 300; 518) Detect Cathepsin B (CaB), [67] caspase-3 [75] /E. coli outer membrane protease (OmpT) [72] /protein/ribosome conformation [76] QSY9 562 500-600 88 000…”

“…In addition, QSY molecules have been widely used as quenchers in FRET‐based fluorogenic probes in the detection of various biological events and molecules, such as guanine, [68] cysteine cathepsin (CTS), [69] cathepsin S, [70] pH, [71] E. coli outer membrane protease (OmpT), [72] β‐lactamase (BlaC), [73] bromo‐benzophenanthridinone 968 on glutaminase C (GAC), [74] caspase‐3, [75] protein/ribosome conformation, [76] liposome, [77] MMP‐2, [78] intracellular hypoxia, [79] actin, [80] myosin, [81] glucose, [82] granzyme B, [83] Botulinum Neurotoxin Serotype E, [84] and phosphatidylcholine‐specific phospholipase C [85] …”

Visualization of Phototherapy Evolution by Optical Imaging

“…Recently, Yu et al reported a lysosome-targeted and self-monitoring probe, NSLN, based on lysosome-to-nucleus immigration ( Figure 10 d) [ 72 ]. Initially, NSLN stained lysosomes in cancer cells, but immigrated into the nucleus through binding with DNA after PDT-induced apoptosis and lysosome damage.…”