Energy metabolic enzyme responses of Litopenaeus vannamei to thermal stress: a comparative study in freshwater and seawater conditions

Jia, Xuying; Zhong, Dasen; Zhang, Dan; Wang, Fang; Zhou, Wenli

doi:10.1007/s10499-018-0268-9

Cited by 14 publications

(7 citation statements)

References 35 publications

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“…Aerobic respiration occupies the main position, but under low salt stress, anaerobic respiration can also provide energy for the body. SDH is an important part of mitochondrial inner membrane and plays a key role as the only enzyme in tricarboxylic acid cycle (TCA) (Jia et al, 2018). SDH activity can affect oxidative phosphorylation, so it reflects the level of aerobic metabolism to some extent (Wang et al, 2017;Jia et al, 2018).…”

Section: Fitness Performancementioning

confidence: 99%

“…SDH is an important part of mitochondrial inner membrane and plays a key role as the only enzyme in tricarboxylic acid cycle (TCA) (Jia et al, 2018). SDH activity can affect oxidative phosphorylation, so it reflects the level of aerobic metabolism to some extent (Wang et al, 2017;Jia et al, 2018). The results of this study showed that at 1.5 h, the SDH of red P. fucata under high and low salinity stress was significantly higher than that of the control group, which was contrary to the results of Nie et al's (2018) study on Dosinia corrugate.…”

Section: Fitness Performancementioning

confidence: 99%

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Physical Responses of Pinctada fucata to Salinity Stress

Yang

Chen

et al. 2022

Front. Mar. Sci.

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This study was conducted to understand the changes of physiological and biochemical indexes of black and red shell Pinctada fucata under acute high and low salt stress. In this study, the salinity of 35‰ was used as the control, while the salinities of 20 and 50% salinity were used as the low and high salt treatment groups, respectively. The osmotic pressure (OSM) and ion concentration in the hemolymph, Na+-K+ -ATPase (NKA) activity and respiratory metabolism in gills, and antioxidant and immune (non) enzymes in the hepatopancreas of P. fucata with two shell colors were compared and analyzed at the time periods of 1.5 and 3 h post-salinity stress. The results showed that the OSM and inorganic ion (Na+, Ca2+, and Cl–) concentration in the hemolymph of the black and red P. fucata increased significantly with the increase of salinity after the time periods of 1.5 and 3 h. At 3 h, the black P. fucata NKA activity decreased significantly with the increase of salinity, while red P. fucata reached the highest value at high salinity. The succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) activities of red P. fucata showed U-shaped and inverted U-shaped distributions with the increase of salinity after 1.5 h, respectively. With the increase of salinity, the phenoloxidase (POX) activity of red and black P. fucata showed inverted U-shaped and U-shaped distributions, respectively. The contents of glutathione (GSH) and vitamin C (VC) in black P. fucata decreased significantly with the increase of salinity at 1.5 and 3 h. Red P. fucata GSH and VC reached their maximum value in the 1.5-h low salinity group and 3-h high salinity group. The vitamin E (VE) content in black P. fucata increased significantly with the increase of salinity at 1.5 h, and reached the maximum at 3 h in the control group. Red P. fucata VE reached the maximum at 1.5 and 3 h in the control group. The results obtained from the present study revealed that the sensitivity of P. fucata to salinity varied in shell color. Compared to black P. fucata, red P. fucata responds more quickly to sharp salinity changes, thereby reducing more likely damage. Compared with a high salt environment, P. fucata was more adaptable to the changes of acute low salt environment. The results obtained from the present study provide the physical references for subsequent selective breeding of this species.

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Section: Fitness Performancementioning

confidence: 99%

Section: Fitness Performancementioning

confidence: 99%

Physical Responses of Pinctada fucata to Salinity Stress

Yang

Chen

et al. 2022

Front. Mar. Sci.

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“…This result is consistent with our previous studies on physiological changes (Zhou et al, 2020), which also indicates that the energy metabolism of overwintering S. paramamosain is more vigorous at low salinity. Studies have shown that the HXK and KPYK of L. vannamei were inhibited in freshwater compared with seawater (Jia et al, 2018). Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme in the phosphogluconic acid pathway of another glucose-decomposition pathway apart from the glycolysis pathway and the citric acid cycle, which catalyses the dehydrogenation of glucose 6-phosphate to form gluconic acid 6-phosphate.…”

Section: Daps Involved In Carbohydrate and Energy Metabolismmentioning

confidence: 99%

iTRAQ‐based quantitative proteomic analysis reveals metabolic changes in overwintering Scylla paramamosain at two different salinities

Zhou

Wang

et al. 2021

Aquaculture Research

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“…In our study, HK at 4‰ salinity was significantly down‐regulated (FC = 0.01) compared with the regulation observed at 25‰ salinity after overwintering. Studies have shown HK in Litopenaeus vannamei was more inhibited in freshwater than in seawater (Jia et al, 2018), and knowledge of genes associated with carbohydrate metabolism is helpful to understand the molecular mechanisms occurring in S. paramamosain during overwintering at different salinities.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals hepatopancreatic genes and pathways associated with metabolism responding to water salinities during the overwintering of mud crab Scylla paramamosain

Zhou

et al. 2021

Aquaculture Research

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Overwintering is an important part of the conservation of Scylla paramamosain, and salinity is a primary parameter affecting this species’ metabolism at this particular time. We compared and analysed gene sequences responsible for the metabolism of S. paramamosain at 4‰ versus 25‰ salinities using transcriptome analysis, in order to find out the optimum salinity for S. paramamosain during overwintering. A total of 5895 genes were detected, of which 105 down‐regulated genes and 131 up‐regulated genes were associated with metabolism (p < 0.05). Among these genes related to metabolic pathways (117 pathways), we identified D‐aspartate oxidase, D‐amino acid oxidase, creatine kinase, sarcosine oxidase, citrate synthase, transferase, glycogen synthase, hexokinase, carbonic anhydrase, glutamine synthetase, V‐type H+‐transporting ATPase, 3′‐phosphoadenosine 5′‐phosphosulphate synthase, fatty acid synthase and others. Additionally, the consistency between qRT‐PCR results and transcriptome data reflected the reliability of transcriptome results in the present study. Compared with the 25‰ salinity group, the 4‰ salinity group promoted energy metabolism, amino acid metabolism, lipid metabolism and carbohydrate metabolism by regulating metabolism‐related genes, which made the overwintering S. paramamosain more active in the low‐salt environment, and hepatopancreas consumed more energy to maintain life activities. These results improve our understanding on molecular mechanisms and metabolic pathways associated with salinity changes in S. paramamosain during overwintering. The data presented in this study are also the first to reveal the molecular mechanisms of S. paramamosain during overwintering in response to different salinities at the gene level.

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Energy metabolic enzyme responses of Litopenaeus vannamei to thermal stress: a comparative study in freshwater and seawater conditions

Cited by 14 publications

References 35 publications

Physical Responses of Pinctada fucata to Salinity Stress

Physical Responses of Pinctada fucata to Salinity Stress

iTRAQ‐based quantitative proteomic analysis reveals metabolic changes in overwintering Scylla paramamosain at two different salinities

Transcriptome analysis reveals hepatopancreatic genes and pathways associated with metabolism responding to water salinities during the overwintering of mud crab Scylla paramamosain

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