The ability to implement adiabatic processes in the mesoscale is of key importance in the study of artificial or biological micro-and nanoengines. Microadiabatic processes have been elusive to experimental implementation due to the difficulty in isolating Brownian particles from their fluctuating environment. Here we report on the experimental realization of a microscopic quasistatic adiabatic process employing a trapped Brownian particle. We circumvent the complete isolation of the Brownian particle by designing a protocol where both characteristic volume and temperature of the system are changed in such a way that the entropy of the system is conserved along the process. We compare the protocols that follow from either the overdamped or underdamped descriptions, demonstrating that the latter is mandatory in order to obtain a vanishing average heat flux to the particle. We provide analytical expressions for the distributions of the fluctuating heat and entropy and verify them experimentally. Our protocols could serve to implement the first microscopic engine that is able to attain the fundamental limit for the efficiency set by Carnot. [15,17,18].Until now, the design of microscopic heat engines has been restricted to those cycles formed by isothermal processes or instantaneous temperature changes [16], where the validity of a heat fluctuation theorem has been tested [19]. Recent works have shown that exerting random forces on a microscopic particle one can accurately tune the effective kinetic temperature of the particle both under equilibrium [20][21][22] and nonequilibrium driving [23]. However, the application of such a technique to implement nonisothermal processes has not been fully exploited yet [24].Among all the nonisothermal processes, adiabatic processes are of major importance in thermodynamics since they are the building blocks of the Carnot engine [25]. Microadiabaticity, i.e., true adiabaticity (TA) at the microscopic scale, cannot be realized for single trajectories due to the unavoidable heat flows between microscopic systems and their surroundings. However, a process where no net heat transfer is obtained when averaged over many trajectories, or mean adiabatic (MA) could, in principle, be realized. For simplicity, we will refer in the following MA processes as adiabatic processes.The notion of microadiabaticity has been studied theoretically since the first models of microscopic heat engines [26]. Schmiedl and Seifert devised a Brownian heat engine with two instantaneous steps in which the positional Shannon entropy of the system is conserved [27]. Further theoretical developments have considered the case of adiabatic processes in the underdamped limit [28,29]. The first experimental studies of microscopic heat engines [16] and nonisothermal processes [19] have not realized the case of adiabatic processes in the mesoscale yet.In this Letter, we report on the realization of quasistatic adiabatic processes with an optically trapped microparticle whose kinetic temperature is controlled by means ...