Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat

Paltridge, Nicholas G.; Milham, Paul J.; Ortiz‐Monasterio, J. Iván; Govindan, Velu; Yasmin, Zarina; Palmer, Lachlan; Guild, Georgia E.; Stangoulis, James

doi:10.1007/s11104-012-1423-0

Cited by 128 publications

(99 citation statements)

References 19 publications

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“…Another possibility of using pXRF is the direct measurement of the elemental composition of plant tissues (leaves) and grains by using pXRF (McLaren;Guppy;Tighe, 2012;Paltridge et al, 2012). Towet, Shephred and Drake (2015) evaluated the composition of leaves of several plants by using pXRF and found a high correlation (R 2 > 0.90) for Mg, P, S, K, Ca and Mn contents with those obtained by the traditional acid digestion followed by determination by ICP-OES.…”

Section: Agronomic Applicationsmentioning

confidence: 99%

Portable X-ray fluorescence (pXRF) applications in tropical Soil Science

Ribeiro

Silva

et al. 2017

Ciênc. agrotec.

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The X-ray fluorescence (XRF) is an analytical technique for determination of elemental composition of different materials. In soils, the XRF has many pedological, environmental and agronomic applications, mainly after the emergence of portable equipments (pXRF). This technique has been recently adopted and successfully used for soil characterization worldwide, but very rare works have been carried out in soils of developing countries. The soil characterization includes the complete elemental composition determination (nutrients, trace and rare-earth elements) and allows estimating some soil physical and chemical properties. In Brazil, this technique is still incipient, mainly the use of pXRF, however, it can greatly contribute to soil characterization in-field or in-lab conditions and also replacing methods of soil analyses considered non-environmentally friendly. This review summarizes the XRF technique including principles and the main applications of pXRF in soils highlighting its potential for tropical Soil Science.Index terms: Soil analyses; soil morphometrics; soil characterization. RESUMOFluorescência de raios-X (FRX) é uma técnica analítica para determinação da composição elementar de diferentes materiais. Em solos, a FRX apresenta muitas aplicações pedológicas, ambientais e agronômicas, principalmente após a emergência de equipamentos portáteis (pXRF). Essa técnica tem sido utilizada com sucessso no mundo todo para caracterização do solo, entretanto, são raros os trabalhos em solos de países em desenvolvimento. A caracterização do solo inclui a determinação completa da composição elementar (nutrientes, elementos-traço e terras-raras) e permite a estimativa de atributos químicos e físicos do solo. No Brasil, a FRX é ainda incipiente, principalmente o uso do pXRF, entretanto, essa técnica pode contribuir grandemente para a caracterização do solo no campo, em condições laboratoriais e, também, substituindo alguns métodos de análise do solo considerados não prejudicial ao ambiente. Esta revisão sumariza a técnica de FRX incluindo princípios e as principais aplicações do pXRF, destacando seu potencial de uso na Ciência do Solo tropical. Termos para indexação:Análise do solo; morfometria do solo; caracterização do solo.

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Section: Agronomic Applicationsmentioning

confidence: 99%

Portable X-ray fluorescence (pXRF) applications in tropical Soil Science

Ribeiro

Silva

et al. 2017

Ciênc. agrotec.

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“…For environmental samples such as soils and plants, with large elemental compositions, the presence and predominance of other elements can interfere with the values for elements of interest. These are known as matrix effects and are often overcome by calibrating using simple matrices or synthetic materials spiked with a range of element concentrations (Paltridge et al, 2012;Reidinger et al, 2012). Alternatively, calibration of complex matrices often uses the fundamental parameters (FP) method, first developed in the mid-50s by Sherman (1955).…”

Section: Overviewmentioning

confidence: 99%

“…The scatter plots between methods indicate good association or correlation between methods for all elements, with points lying along a straight line describing the relationship between methods. This approach has been used to compare EDXRF measurements on wheat with ICP values for zinc, iron and selenium (Paltridge et al, 2012). However, relying on correlation and regression coefficients to describe agreement between methods can be misleading (Altman and Bland, 1983;Bland and Altman, 1986;Bland and Altman, 2003) as perfect agreement exists if points lie along the line of equality but perfect correlation exists if points lie along any straight line.…”

Section: Repeatability and Robustness Testingmentioning

confidence: 99%

“…The amount of K required for good-quality grass in pasture-based systems is estimated as 2.5% DM, although K deficiencies in animals are rarely reported. Given the highly variable nature of nutrients in Irish grass depending on the stage of growth (Fleming and Murphy, 1968) and soil type (Fleming et al, 1963), elemental analysis of grass is a useful during the remediation of contaminated soils; however, more recently, the use of EDXRF has provided comparable results with conventional methods for the determination of various elements in medicinal plants (Queralt et al, 2005;Elzain et al, 2016), as well as some trace elements in wholegrain wheat (Paltridge et al, 2012) and fodder (Necemer et al, 2003), using synthetic calibrators and certified reference materials (CRMs) as calibration standards. The main objective of this work was to evaluate EDXRF as a non-destructive method for determining P, K, Mg and Ca in grass samples obtained from Irish dairy farms.…”

Section: Introductionmentioning

confidence: 99%

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A rapid and multi-element method for the analysis of major nutrients in grass (Lolium perenne) using energy-dispersive X-ray fluorescence spectroscopy

Daly

Fenelon

2017

Irish Journal of Agricultural and Food Research

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Elemental analysis of grass (Lolium perenne) is essential in agriculture to ensure grass quality and animal health. Energy-dispersive X-ray fluorescence (EDXRF) spectroscopy is a rapid, multi-element alternative to current methods using acid digestion and inductively coupled plasma optical emission spectrometry (ICP-OES). Percentage phosphorus (P), potassium (K), magnesium (Mg) and calcium (Ca), determined from grass samples using EDXRF, were within 0.035, 0.319, 0.025 and 0.061, respectively, of ICP-OES values. Concordance correlation coefficients computed using agreement statistics ranged from 0.4379 to 0.9669 (values close to one indicate excellent agreement); however, the level of agreement for each element depended on the calibrations used in EDXRF. Empirical calibrations gave excellent agreement for percentage P, K and Ca, but moderate agreement for percentage Mg due to a weaker correlation between standards and intensities. Standardless calibration using the fundamental parameters (FP) approach exhibited bias, with consistently lower values reported for percentage P and Mg, when compared with ICP-OES methods. The relationship between the methods was plotted as scatter plots with the line of equality included, and although correlation coefficients indicated strong relationships, these statistics masked the effects of consistent bias in the data for percentage P and Mg. These results highlight the importance of distinguishing agreement from correlation when using statistical methods to compare methods of analysis. Agreement estimates improved when a matching library of grass samples was added to the FP method. EDXRF is a comparable alternative to conventional methods for grass analysis when samples of similar matrix type are used as empirical standards or as a matching library.

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“…In order to perform quantitative measurements, calibration can be done by the linear relationship between the intensity of X-ray emission of a target element and its concentration previously determined by a reference method. The quality of the XRF data can be evaluated by the correlation between the XRF intensity and the reference values using figures of merit such as linear correlation factor, standard error of prediction (SEP), confidence intervals and bias (Paltridge et al 2012). Applying histochemical and chemical techniques for detection of compounds of interest, such as potentially toxic metals, can complement the data obtained from X-ray microanalysis, since they are useful for locating discrete quantities of chemical elements in biological tissues (Pearse 1972, Krishnamurthy 1998.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of micro-energy dispersive X-ray fluorescence and histochemical tests for aluminium detection in plants from High Altitude Rocky Complexes, Southeast Brazil

Campos

Pereira

Machado

et al. 2014

An. Acad. Bras. Ciênc.

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The soils developed under High Altitude Rocky Complexes in Brazil are generally of very low chemical fertility, with low base saturation and high exchangeable aluminium concentration. This stressful condition imposes evolutionary pressures that lead to ecological success of plant species that are able to tolerate or accumulate high amounts of aluminium. Several analytical methods are currently available for elemental mapping of biological structures, such as micro-X-ray fluorescence (µ-EDX) and histochemical tests. The aim of this study was to combine µ-EDX analysis and histochemical tests to quantify aluminium in plants from High Altitude Rocky Complexes, identifying the main sites for Al-accumulation. Among the studied species, five showed total Al concentration higher than 1000 mg kg -1 . The main Al-hyperaccumulator plants, Lavoisiera pectinata, Lycopodium clavatum and Trembleya parviflora presented positive reactions in the histochemical tests using Chrome Azurol and Aluminon. Strong positive correlations were observed between the total Al concentrations and data obtained by µ-EDX analysis. The µ-EDX analysis is a potential tool to map and quantify Al in hyperaccumulator species, and a valuable technique due to its non-destructive capacity. Histochemical tests can be helpful to indicate the accumulation pattern of samples before they are submitted for further µ-EDX scrutiny.

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Energy-dispersive X-ray fluorescence spectrometry as a tool for zinc, iron and selenium analysis in whole grain wheat

Cited by 128 publications

References 19 publications

Portable X-ray fluorescence (pXRF) applications in tropical Soil Science

Portable X-ray fluorescence (pXRF) applications in tropical Soil Science

A rapid and multi-element method for the analysis of major nutrients in grass (Lolium perenne) using energy-dispersive X-ray fluorescence spectroscopy

Evaluation of micro-energy dispersive X-ray fluorescence and histochemical tests for aluminium detection in plants from High Altitude Rocky Complexes, Southeast Brazil

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