Evaluation of micro-energy dispersive X-ray fluorescence and histochemical tests for aluminium detection in plants from High Altitude Rocky Complexes, Southeast Brazil

Campos, Naiara Viana; Pereira, Tiago Augusto Rodrigues; Machado, Mariana; Guerra, Marcelo Braga Bueno; Tolentino, Gláucia Soares; Araújo, Josiane Silva; Rezende, Maíra Queiroz; Silva, Maria Carolina N.A. Da; Schaefer, Carlos Ernesto Gonçalves Reynaud

doi:10.1590/0001-3765201402012

Cited by 9 publications

(6 citation statements)

References 46 publications

(40 reference statements)

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“…The majority of morin-stainable Al in tea is accumulated in the root tips, root rhizodermal cells and root hairs and basal parts show considerably lower Al accumulation (Hajiboland and Poschenrieder 2015). A steep Al concentration gradient has been also observed among midvein and interveinal regions in the leaves of some Al accumulators (Campos et al 2014).…”

Section: Interaction Between Al and B At The Level Of Allocation And mentioning

confidence: 91%

Interactions between aluminum and boron in tea (Camellia sinensis) plants

et al. 2015

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Response of tea plants (Camellia sinensis (L.) O. Kontze) to Al (300 lM AlCl 3 for 14 weeks) was studied in combination with deficient (-B) or adequate B supply (?B) in hydroponics. Aluminum improved plant growth under B deficiency. This positive Al effect in (-B) plants was related to an Al-induced increase of B contents in the root cell walls (CW). Moreover, in (-B) plants more Al was partitioned into CW-bound fractions in both leaves and roots than in (?B) plants; an indication that B deficiency reduced the mobility of Al in the tea plants. In general, the highest activities of phenylalanine ammonia lyase, polyphenol oxidase, and soluble and CW-bound fractions of peroxidases were observed in (?Al/-B) plants. In (-B) plants Al supply caused a reduction of CW-bound phenolic acids and lignin, while the concentrations of soluble phenolics increased in the leaves. In the roots, however, Al treatment of B-deficient plants caused a significant increase of CW-bound phenolic acids, but not of lignin. Our results suggest that increased B partitioning into CW and reduction of lignification were important causes for Al-mediated amelioration of growth in B-deficient plants. In addition, the observation that in (?Al/-B) roots CW binding of both Al and phenolic acids was enhanced indicates that in the B-deficient roots Al was mainly bound to the CW phenolic acids; this, in turn, reduced their availability for enzymatic reactions and lignin synthesis.

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Section: Interaction Between Al and B At The Level Of Allocation And mentioning

confidence: 91%

Interactions between aluminum and boron in tea (Camellia sinensis) plants

et al. 2015

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“…Energy dispersive X‐ray fluorescence has also been useful in studies on elemental composition of plants and in identifying potentially hazardous trace metals in plants . Micro‐ED‐XRF, which can apply a spot size of only a few microns to provide cellular‐level resolution, has been used to identify the deposition of elements within plant tissue . In a comparison study with inductively‐coupled plasma‐optical emission spectrometry (ICP‐OES), data obtained through ED‐XRF did not deviate more than 2 mg kg −1 for either Fe or Zn in rice and pearl millet grain .…”

Section: Introductionmentioning

confidence: 99%

“…A pXRF unit can be used under either field or laboratory conditions, and considerations should be given to which approach will yield optimal results for the investigator. Numerous research studies e.g . have extolled the virtues of using XRF for wide range of plant and environmental science applications.…”

Section: Introductionmentioning

confidence: 99%

Plant elemental composition and portable X‐ray fluorescence (pXRF) spectroscopy: quantification under different analytical parameters

2015

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Emergence of portable X-ray fluorescence (pXRF) systems presents new opportunities for rapid, low-cost plant analysis, both as a lab system and in situ system. Numerous studies have extolled the virtues of using pXRF for a wide range of plant applications, however, for many such applications, there is need for further assessment with regards to analytical parameters for plant analysis. While pXRF is a potential powerful research tool for elemental composition analysis, its successful use in plant analysis is made more likely by having an understanding of X-ray physics, calibration process, and ability to test a variety of homogenous and well-characterized materials for developing a matrix-specific calibration. Because potential pXRF users may often underestimate the complexity of proper analysis, this study aims at providing a technical background for plant analysis using pXRF. The focus is on elemental quantification under different analytical parameters and different methods of sample presentation: direct surface contact under vacuum, placement in a sample cup with prolene as a seal, and without the aid of a vacuum. Direct analysis on the surface of a pXRF provided highest sensitivity and accuracy (R 2 > 0.90) for light elements (Mg to P). Sulfur, K, and Ca can be reliably measured without the aid of a vacuum (R 2 > 0.99, 0.97, and 0.93 respectively), although lower detection limits may be compromised. pXRF instruments provide plant data of sufficient accuracy for many applications and will reduce the overall time and budget compared with the use of conventional techniques. Sensitivity and accuracy are dependent on the instrument's settings, make, and model. © 2015 The Authors. X-Ray Spectrometry published by John Wiley & Sons, Ltd.Additional supporting information may found in the online version of this article at publisher's web site. IntroductionKey plant nutrients, including potassium (K), phosphorus (P), and sulfur (S), play important roles in regulating processes such as photosynthesis, carbon respiration, and tissue building. Elemental analysis of plants is critical to plant studies and agriculture. However, despite the routine application of digestion-based analytical techniques in many laboratories, the slow and often hazardous sample digestion process can present a challenge in the analyses of plant nutrients, particularly where hundreds or even thousands of samples are to be analysed.[1] The conventional plant elemental analyses based on plant extracts are cumbersome and of variable accuracy and present major limitations to exploiting the full potential of plant nutrient analysis. An ideal system for analysis would include accuracy, replicability, portability, and minimal sample preparation. The technology that currently provides the best option to meet these needs is energy-dispersive X-ray fluorescence (ED-XRF), particularly in the form of a portable instrument portable X-ray fluorescence (pXRF). These instruments are commonly used for scrap metal sourcing, [2] geochemical provenience of obsidian,...

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“…This result is in line with an earlier, preliminary comparison between the aluminon test and exact Al concentrations for 10 Rubiaceae species (Jansen et al, 2003). Furthermore, the test solution is also used as a dye for studying the localization of high Al levels in plant tissues (Haridasan et al, 1986; Haridasan, 2008; Campos et al, 2014). However, Clark and Krueger (1985) critically investigated the aluminon solution with regards to other elements and reported that staining patterns similar to Al can be found for other elements, especially Fe.…”

Section: Discussionmentioning

confidence: 99%