The growing interest in alternative fuels based on plant oils has led to the search for new plant species. Given this, during 2015-2020, oil from 12 varieties of oilseed radish was studied using standard research protocols. The average content of the dominant fatty acids in the oils studied was: [cis-9] oleic (C 18:1) 33.95% (Cv = 14.2%), [cis-9,12] linoleic (C 18:2) 16.20% (Cv = 20.8%), [cis-13] erucic (C 22:1) 15.18% (Cv = 17.9%), [cis-9, 12, 15] α-linolenic (C 18:3) 13.33% (Cv = 18.5%) and palmitic (C 16:0) 5.42% (Cv = 18.5%), with a monounsaturated fatty acid content of 59.69% and a ratio of polyunsaturated/monounsaturated fatty acids of 0.508. The studied varieties were ranked in the order of increasing suitability as a component of biofuels: 'Zhuravka' < 'Raiduha' < 'Lybid' < 'Olga' < 'Iveya' < 'Ramonta' < 'Alpha' < 'Tambovchanka' < 'Fakel' < 'Snizhana' < 'Sabina' < 'Nika'. The technological suitability of oil from the 'Zhuravka' variety was confirmed based on analysis of its physicochemical parameters when subjected to polymerization (at 280 °C) and oxypolymerization (at 120 and 150 °C). Under these conditions the basic parameters of the oil varied within the technological limits that determine its suitability for thermodynamic combustion processes in systems with controlled pressure and temperature.