Energization of lymphocytes transforming to macrophages in human inflammation

Rebuck, John W.; Whitehouse, Fred W.; Noonan, Sharon M.

doi:10.1016/0006-2952(68)90303-1

Cited by 7 publications

(7 citation statements)

References 21 publications

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“…Furthermore, initial passage of the unfractionated cell suspension through a column sensitized with a non cross-reacting antigen (Table XII) or an unsensitized glass bead column (Table XIII) prior to passage of the cells through a specifically sensitized column did not diminish the effectiveness of the eluate cells to transfer specific immunocompetence to irradiated recipient rabbits. Since the initial passage of the cells should have depleted the cell suspension of macrophages or seriously reduced their number (17)(18)(19), it is obvious that the functionally active macrophage does not participate in the interaction between the antigenreactive cell and the antigen adsorbed onto the glass beads. Although one might be tempted to rule out any role for the macrophage in the induction of the primary response in the irradiated recipient rabbit, it would be necessary to establish unequivocally that the exposure to 800 R irradiation killed all the macrophages and their precursor cells in vivo and that no macrophages were transferred with the eluate cells.…”

Section: Discussionmentioning

confidence: 99%

“…Although macrophages could not be distinguished morphologically in the eluate population of cells, it is possible, because of the manipulation of the cells, that they had assumed a different appearance. Furthermore, it has been demonstrated that lymphocytes can transform to macrophages (19), thus rendering academic the entire question of the role of the macrophage.…”

Section: Discussionmentioning

confidence: 99%

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Cells Involved in the Immune Response

Abdou

Richter

1969

The Journal of Experimental Medicine

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The antigen-reactive cells in normal rabbit bone marrow could be isolated from a suspension of marrow cells by passage of the cells through an antigen-sensitized glass bead column. The cells which passed through the column were deficient in antigen-reactive cells directed to the antigen used to sensitize the glass beads, whereas the cells eluted from the column could transfer antibody-forming capacity to irradiated recipients only with respect to the specific sensitizing antigen. Separation of the bone marrow antigen-reactive cells could not be achieved by passage of the cells through nonsensitized glass bead columns or in the presence of excess free antigen in the column. Cells which were retained by, and later eluted from, the antigen-sensitized glass bead columns were mostly small mononuclear cells, whereas cells which passed through the columns were morphologically similar to the original unfractionated bone marrow cell suspension. The data indicate the presence of an antibody or antibody-like structure, with defined immunological specificity, on the surface of the normal bone marrow antigen-reactive cell.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cells Involved in the Immune Response

Abdou

Richter

1969

The Journal of Experimental Medicine

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“…This would occur through the same mechanisms described for the transfer of other neutrophil components, like peroxidase (Afonso et al 1998b). It is tempting to consider that the glycogen particles transferred from neutrophils to macrophages would be used by the macrophages for their own metabolic activities, as was suggested by Rebuck et al (1968). …”

Section: A B Cmentioning

confidence: 97%

Glycogen granules in resting and inflammatory rainbow trout phagocytes-an ultrastructural study

Afonso¹,

Macedo²,

Ellis³

et al. 2000

Dis. Aquat. Org.

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“…Inflammatory PMNs accumulate increased amounts of glycogen (Wulff, 19t32;Rebuck et al, 1968;Robinson et al, 1982). Its role is presumed to be as an energy stare to be utilized during phagocytosis (Scott, 1968).…”

mentioning

confidence: 99%

“…Cklycogen is a prominent and dynamic constituent of the cytoplasm of polymorphonuclew leukocytes (PMNs) of various species (Sbarra and Karnovsky, 1959;Abul-Fad1 and Scott, 1969). Inflammatory PMNs accumulate increased amounts of glycogen (Wulff, 19t32;Rebuck et al, 1968;Robinson et al, 1982). Its role is presumed to be as an energy stare to be utilized during phagocytosis (Scott, 1968).…”

mentioning

confidence: 99%

Glycogen autophagosomes in polymorphonuclear leukocytes induced by rickettsiae

Rikihisa

1984

Anat. Rec.

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Guinea pig polymorphonuclear leukocytes (PMNs), rich in glycogen granules, were collected from sodium-caseinate-induced peritoneal exudate. When these cells were incubated with rickettsiae, many microorganisms were phagocytized within 30 minutes at 35 degrees C and vacuoles up to 5 microns in diameter containing glycogen granules were present. Contained within these vacuoles were phagocytized extracellular material and a dense, lysosomelike substance that was acid phosphatase positive. These vacuoles, which were interpreted to be autophagosomes, were absent from PMNs that had not been stimulated with microorganisms. The number of rickettsiae in the PMN did not appear to be related to the number of autophagosomes. About 8% and 80% of thin-sectioned profiles of PMNs contained these vacuoles after 30 minutes and 4 hours incubation, respectively. After 4 hours, the PMNs contained multiple autophagosomes. Almost all of the glycogen granules were in autophagosomes in some of the cells. In some PMNs, discontinuous membranes encircled some glycogen. When PMNs were initially incubated with thorium dioxide and ferritin, and extensively washed prior to incubation with rickettsiae, glycogen was found surrounded by flattened secondary lysosomes containing the dense tracers. Some autophagosomes also contained the electron-dense tracers. These results suggest that rickettsiae induce the rapid formation of glycogen-containing autophagosomes in guinea pig peritoneal PMNs in vitro.

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Energization of lymphocytes transforming to macrophages in human inflammation

Cited by 7 publications

References 21 publications

Cells Involved in the Immune Response

Cells Involved in the Immune Response

Glycogen granules in resting and inflammatory rainbow trout phagocytes-an ultrastructural study

Glycogen autophagosomes in polymorphonuclear leukocytes induced by rickettsiae

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