Glycogen granules in resting and inflammatory rainbow trout phagocytes-an ultrastructural study

Kinetics of Cellular Component in Inflammatory Response Induced by Different Stimuli in the Swim Bladder of Pacu, Piaractus mesopotamicus Holmberg 1887 (Characidae)

“…In macrophages from inflammatory exudate from pacu, in this study, glycogen granules were not identified after application of the PAS method as was reported in the rainbow trout (Afonso et al 2000). However, in exudate from sea bass induced with Photobacterium damselae , macrophages and neutrophils showed evidence of glycogen granules, making the distinction of these cell populations by light microscopy difficult.…”

Measurement of in vitro leucocyte mitogenesis in fish: ELISA based detection of the thymidine analogue 5′-bromo-2′-deoxyuridine

“…1). Background may also be derived from endogenous peroxidase activity within certain leucocytes [33] and background may be pronounced when erythrocytes, which possess peroxidase activity [34], contaminate the leucocyte cultures. This type of background is independent of BrdU incorporation but has the same end e#ect.…”

A Quantitative Analysis of the Subcellular Distribution of Human Skeletal Muscle Glycogen

“…This combination therefore facilitates the analysis of intra-muscular glycogen by enhancing its contrast compared to other cellular components. Indeed, an analysis of the influence of sample processing methods in electron microscopy on the ultrastructure of glycogen particles was recently performed in animal tissues (Afonso 2000). The results confirmed that, compared to other preparations, the highest electron density of glycogen granules was obtained when osmium tetroxide fixative was supplemented with potassium ferricyanide.…”