ENDOTOXIN-INDUCED TOLERANCE TO TOXIC MANIFESTATIONS OFCANDIDA ALBICANS

Abstract

Hasenclever , H. F. (National Institutes of Health, Bethesda, Md.) and William O. Mitchell . Endotoxin-induced tolerance to toxic manifestations of Candida albicans . J. Bacteriol. 85: 1088–1093. 1963.—Mice exposed to 425 r total body irradiation failed to become tolerant to Candida albicans toxicity after injection with lipopolysaccharide. Mice injected with lipopolysaccharide and then X-rayed did not demonstrate the tolerant state. An injection of thorium dioxide in mice that had previously received tolerance-inducing amounts of lipopolysaccharide rendered them as susceptible to acute C. albicans toxicity as control mice. A bimodal manifestation of tolerance was noted. Groups of mice given single injections of lipopolysaccharide at 6 or 1 days before challenge demonstrated high levels of tolerance, whereas the tolerance in mice given a single dose at 3 days was negligible. The bimodal effect was not observed in tolerant mice challenged with lipopolysaccharide. Injections of viable or nonviable pathogenic fungi known to produce tolerance to the toxicity of C. albicans in recipient mice did not produce tolerance to lipopolysaccharide. Serum from mice injected with lipopolysaccharide showed in vitro inhibitory activity for C. albicans .

Acquired Immunity to Candidiasis in Mice

“…There was some heterologous response, but the specific response was stronger. Although our work (Hasenclever and Mitchell, 1963) showed that S. enteritidis endotoxin stimulated mice to produce serum-inhibitory substances for C. albicans, antigenic similarity between these two microorganisms is not known to exist. More investigation is necessary to elucidate the nature of these inhibitory factors found in the sera of mice after injection of endotoxin, C. immitis s1)herule fragments, or viable C.…”

“…The presence of C. albicans growth-inhibiting substances in the sera of C. immitis-injected mice may also contribute to the state of acquired resistance. Other studies with sera obtained from mice injected with endotoxin indicated the presence of similar inhibitory factors (Hasenclever and Mitchell, 1963). Whitby et al (1961) reported the formation, in the sera of mice, of specific-lytic antibodies to gram-negative bacteria after the injection of endotoxin.…”