Endotoxin-induced cytokine, chemokine and white blood cell profiles of variable stress-responding sheep

Naylor, Danielle; Sharma, Ankita; Li, Z.; Monteith, Gregory R.; Mallard, Bonnie A.; Bergeron, Renée; Baes, C.F.; Karrow, Niel A.

doi:10.1080/10253890.2021.1954905

Cited by 4 publications

(7 citation statements)

References 45 publications

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“…On the other hand, miRNA-29b was higher in HSR animals compared to the LSR group, and it is known to regulate glucose metabolism and natural killer cell function and is involved in the inhibition of the Th1-cell-mediated immune response [38,39]. These results agree well with the high levels of anti-inflammatory cytokines (IL-10 and IL-4) in the HSR group [12]. Moreover, the high abundance of miRNA-29 expression was previously associated with high-performance dairy cows and is known to increase the availability of branched-chain essential amino acids, hence activating the mTOR signaling pathway [40].…”

Section: Discussionsupporting

confidence: 79%

“…This study reported that miRNA-411 attenuated NFκB, thus improving inflammation via JNK pathway inhibition by negatively targeting the pro-inflammatory cytokine IL-18 [31]. Our previous study showed lower cortisol and pro-inflammatory cytokine levels in the LSR group [12], which aligns well the with high expression of miR-411.…”

Section: Discussionsupporting

confidence: 63%

“…Variation in stress responsiveness is determined by genetics and epigenetic changes and is associated with variation in the immune response. For example, Naylor et al demonstrated different cytokine levels and leukocyte numbers in variable stress-responding sheep following an LPS immune challenge [12]. These variable responses to an LPS immune challenge could present an effective strategy to increase the stress resiliency of sheep through genetic selection.…”

Section: Discussionmentioning

confidence: 99%

“…The stimulation of the HPA axis by exposure to LPS leads to the activation of the systemic acute-phase response, which is well studied [1,3,[9][10][11]. Our previous ovine studies have characterized the LPSinduced acute stress response of lambs and demonstrated variability in individual stress responses [3,11,12]. LPS is a principal pathogen-associated molecular pattern (PAMP) primarily recognized by Toll-like receptor 4 (TLR4), which is expressed on mammalian leukocytes.…”

Section: Introductionmentioning

confidence: 99%

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Expression Profile of miRNA from High, Middle, and Low Stress-Responding Sheep during Bacterial Endotoxin Challenge

Shandilya

Sharma

Naylor³

et al. 2023

Animals

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Animals respond to stress by activating a wide array of physiological and behavioral responses that are collectively referred to as the stress response. MicroRNAs (miRNAs) are small, noncoding RNAs that play key roles in the regulation of homeostasis. There are many reports demonstrating examples of stress-induced miRNA expression profiles. The aim of this study was to determine the circulatory miRNA profile of variable stress-responding lambs (n = 112) categorized based on their cortisol levels as high (HSR, 336.2 ± 27.9 nmol/L), middle (MSR, 147.3 ±9.5 nmol/L), and low (LSR, 32.1 ± 10.4 nmol/L) stress responders post-LPS challenge (400 ng/kg iv). Blood was collected from the jugular vein at 0 (T0) and 4 h (T4) post-LPS challenge, and miRNAs were isolated from four animals from each group. An array of 84 miRNAs and 6 individual miRNAs were evaluated using qPCR. Among 90 miRNAs, there were 48 differentially expressed (DE) miRNAs (log fold change (FC) > 2 < log FC) in the HSR group, 46 in the MSR group, and 49 in the LSR group compared with T0 (control) samples. In the HSR group, three miRNAs, miR-485-5p, miR-1193-5p, and miR-3957-5p were significantly (p < 0.05) upregulated, while seven miRNAs, miR-376b-3p, miR-376c-3p, miR-411b-5p, miR-376a-3p, miR-376b-3p, miR-376c-3p, and miR-381-3p, were downregulated (p < 0.05) as compared to the LSR and MSR groups. Functional analysis of DE miRNAs revealed their roles in Ras and MAPK signaling, cytokine signaling, the adaptive immune system, and transcription pathways in the HSR phenotype, implicating a hyper-induced acute-phase response. In contrast, in the LSR group, enriched pathways included glucagon signaling metabolic regulation, the transportation of amino acids and ions, and the integration of energy metabolism. Taken together, these results indicate variation in the acute-phase response to an immune stress challenge, and these miRNAs are implicated in regulating responses within cortisol-based phenotypes.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression Profile of miRNA from High, Middle, and Low Stress-Responding Sheep during Bacterial Endotoxin Challenge

Shandilya

Sharma

Naylor³

et al. 2023

Animals

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“…We have shown, for example, that transient inflammation lasting approximately 6 h, elicited by an immune challenge with Escherichia coli LPS endotoxin at gestation day 135, is sufficient to alter male and female offspring immune responsiveness at 4.5 months of age [142], and neuroendocrine responsiveness at 5.5 months of age [143,144]. Using this endotoxin model, we have explored candidate protein and miRNA biomarkers for assessing the acute-phase response (APR) to immune challenge [145,146], and characterized variations in the APR at the population level [147], showing a moderately heritable phenotype [148].…”

Section: Using An Ovine Model To Study Long-term Outcomes Of Maternal...mentioning

confidence: 99%

Maternal COVID-19 Vaccination and Its Potential Impact on Fetal and Neonatal Development

et al. 2021

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Vaccines have been developed at “warp speed” to combat the COVID-19 pandemic caused by the SARS-CoV-2 coronavirus. Although they are considered the best approach for preventing mortality, when assessing the safety of these vaccines, pregnant women have not been included in clinical trials. Thus, vaccine safety for this demographic, as well as for the developing fetus and neonate, remains to be determined. A global effort has been underway to encourage pregnant women to get vaccinated despite the uncertain risk posed to them and their offspring. Given this, post-hoc data collection, potentially for years, will be required to determine the outcomes of COVID-19 and vaccination on the next generation. Most COVID-19 vaccine reactions include injection site erythema, pain, swelling, fatigue, headache, fever and lymphadenopathy, which may be sufficient to affect fetal/neonatal development. In this review, we have explored components of the first-generation viral vector and mRNA COVID-19 vaccines that are believed to contribute to adverse reactions and which may negatively impact fetal and neonatal development. We have followed this with a discussion of the potential for using an ovine model to explore the long-term outcomes of COVID-19 vaccination during the prenatal and neonatal periods.

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Common mechanisms underlying diabetic vascular complications: focus on the interaction of metabolic disorders, immuno-inflammation, and endothelial dysfunction

Xue,

Chen,

Gao

et al. 2023

Cell Commun Signal

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Diabetic vascular complications (DVCs), including macro- and micro- angiopathy, account for a high percentage of mortality in patients with diabetes mellitus (DM). Endothelial dysfunction is the initial and role step for the pathogenesis of DVCs. Hyperglycemia and lipid metabolism disorders contribute to endothelial dysfunction via direct injury of metabolism products, crosstalk between immunity and inflammation, as well as related interaction network. Although physiological and phenotypic differences support their specified changes in different targeted organs, there are still several common mechanisms underlying DVCs. Also, inhibitors of these common mechanisms may decrease the incidence of DVCs effectively. Thus, this review may provide new insights into the possible measures for the secondary prevention of DM. And we discussed the current limitations of those present preventive measures in DVCs research.

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Endotoxin-induced cytokine, chemokine and white blood cell profiles of variable stress-responding sheep

Cited by 4 publications

References 45 publications

Expression Profile of miRNA from High, Middle, and Low Stress-Responding Sheep during Bacterial Endotoxin Challenge

Expression Profile of miRNA from High, Middle, and Low Stress-Responding Sheep during Bacterial Endotoxin Challenge

Maternal COVID-19 Vaccination and Its Potential Impact on Fetal and Neonatal Development

Common mechanisms underlying diabetic vascular complications: focus on the interaction of metabolic disorders, immuno-inflammation, and endothelial dysfunction

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