Endothelium and biomaterials: Morpho-functional assessments

Henze, U.; Kaufmann, M.; Klein, Barbara E. K.; Handt, Stefan; Klosterhalfen, B.

doi:10.1016/s0753-3322(96)89681-8

Cited by 6 publications

(5 citation statements)

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“…An alternative to homografts are synthetic grafts [3][4][5][6]. Synthetic grafts generally have a lower level of patency because practically all materials are somewhat thrombogenic and clogging of implanted grafts is a common problem [7]. To solve this problem, many different approaches, including a variety of anti-thrombogenic coatings [6,8] and endothelial cell seeding [9,10], have been tried.…”

Section: Introductionmentioning

confidence: 99%

Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Zorlutuna¹,

Rong²,

Vadgama³

et al. 2009

Acta Biomaterialia

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Section: Introductionmentioning

confidence: 99%

Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Zorlutuna¹,

Rong²,

Vadgama³

et al. 2009

Acta Biomaterialia

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“…When autografts are not available, synthetic grafts made from Dacron, expanded poly(tetrafluoroethylene) (ePTFE), or polyurethane are used. Synthetic vascular grafts are freely available but clogging, compliance mismatch, and fibrous tissue formation are major problems leading in some cases to graft failure . Different approaches including antithrombogenic coatings, endothelial cell seeding, , and modification of pore size and surface texture have been tried to solve them. − Even though these approaches improved the performance of the synthetic grafts, they were still limited to the treatment of large arteries (>6 mm inner diameter).…”

Section: Introductionmentioning

confidence: 99%

Nanopatterning of Collagen Scaffolds Improve the Mechanical Properties of Tissue Engineered Vascular Grafts

2009

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Tissue engineered constructs with cells growing in an organized manner have been shown to have improved mechanical properties. This can be especially important when constructing tissues that need to perform under load, such as cardiac and vascular tissue. Enhancement of mechanical properties of tissue engineered vascular grafts via orientation of smooth muscle cells by the help of topographical cues have not been reported yet. In the present study, collagen scaffolds with 650, 500, and 332.5 nm wide nanochannels and ridges were designed and seeded with smooth muscle cells isolated from the human saphenous vein. Cell alignment on the construct was shown by SEM and fluorescence microscopy. The ultimate tensile strength (UTS) and Young's modulus of the scaffolds were determined after 45 and 75 days. Alamar Blue assay was used to determine the number of viable cells on surfaces with different dimensioned patterns. Presence of nanopatterns increased the UTS from 0.55 +/- 0.11 to as much as 1.63 +/- 0.46 MPa, a value within the range of natural arteries and veins. Similarly, Young's modulus values were found to be around 4 MPa, again in the range of natural vessels. The study thus showed that nanopatterns as small as 332.5 nm could align the smooth muscle cells and that alignment significantly improved mechanical properties, indicating that nanopatterned collagen scaffolds have the potential for use in the tissue engineering of small diameter blood vessels.

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“…Up to now, meeting this challenge has been mostly attempted by fabricating porous scaffolds [178], which provides sufficient space for vascularization. However, this approach cannot overcome the vascularization challenge completely due to the diffusion of cells and other materials into these porous structures [179]. Evidently, advances in bioprinting technologies will provide potential solutions to overcome this problem while forming interconnected, well-defined vascular structures during biomanufacturing process.…”

Section: Future Perspective and Challengesmentioning

confidence: 99%

Towards artificial tissue models: past, present, and future of 3D bioprinting

Arslan‐Yildiz¹,

Assal²,

Chen³

et al. 2016

Biofabrication

254

175

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Regenerative medicine and tissue engineering have seen unprecedented growth in the past decade, driving the field of artificial tissue models towards a revolution in future medicine. Major progress has been achieved through the development of innovative biomanufacturing strategies to pattern and assemble cells and extracellular matrix (ECM) in three-dimensions (3D) to create functional tissue constructs. Bioprinting has emerged as a promising 3D biomanufacturing technology, enabling precise control over spatial and temporal distribution of cells and ECM. Bioprinting technology can be used to engineer artificial tissues and organs by producing scaffolds with controlled spatial heterogeneity of physical properties, cellular composition, and ECM organization. This innovative approach is increasingly utilized in biomedicine, and has potential to create artificial functional constructs for drug screening and toxicology research, as well as tissue and organ transplantation. Herein, we review the recent advances in bioprinting technologies and discuss current markets, approaches, and biomedical applications. We also present current challenges and provide future directions for bioprinting research.

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Endothelium and biomaterials: Morpho-functional assessments

Cited by 6 publications

References 0 publications

Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Influence of nanopatterns on endothelial cell adhesion: Enhanced cell retention under shear stress

Nanopatterning of Collagen Scaffolds Improve the Mechanical Properties of Tissue Engineered Vascular Grafts

Towards artificial tissue models: past, present, and future of 3D bioprinting

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