Endothelin Receptor-Independent Correlation Between HSP47 and Collagen in Rabbit Model of Early Atherosclerosis

Abstract

A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Col la gen in the ex tra cel lu lar mat rix (ECM) plays an im por tant ro le in mo dula tion of res pon se to the vas cu lar in jury du ring the prog res si on of at he rosc le ro sis and res te no sis. Colla gen can re gu la te smo oth musc le cell (SMC) pro li fe ra ti on, mig ra ti on and mat rix me tal lop ro te i na se (MMP) pro duc ti on. The re fo re, col la gen tur no ver in the ar te ri es is an im por tant de ter mi nant of in timal thic ke ning. He at shock pro te in 47 (HSP47), a col la gen-spe ci fic mo le cu lar cha pe ro ne, is tho ught to be es sen ti al for the pro ces sing and sec re ti on of pro col la gen mo le cu les. En dot he lin (ET) is a strong che mo at rac tant and mi to gen pro mo ting SMC pro li fe ra ti on and mig ra ti on. The aim of this study was to in ves ti ga te the pos sib le ro le of HSP47 and its re la ti on to col la gen synthe sis, and the ef fects of a non se lec ti ve ETA/ETB re cep tor an ta go nist, TAK-044 in col lar-in du ced early at he rosc le ro sis mo del. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : New Ze a land whi te rab bits we re di vi ded in to two gro ups. Both gro ups re ce ived ve hic le (0.9% NaCl 0.8 ml/kg/day, s.c.) or TAK-044 (5 mg/kg/day, s.c.) for three we eks. On 8 th day, a non-occ lu si ve si li con col lar was pla ced aro und the left ca ro tid ar tery. The right ca ro tid ar tery was sham-ope ra ted. HSP47 ex pres si on in ca ro tid ar te ri es we re de ter mi ned im mu no his toc he mi cally. Furt her mo re, to tal col la gen le vels, col la gen ex pres si on and type I pro col la gen ex pres si on we re es tab lished. R Re e s su ul lt ts s: : HSP47 ex pres si on cor re la ted with col la gen ex pres si on did not chan ge in col la red ar te ri es. TAK-044 tre at ment did not af fect HSP47 and col la gen le vels. C Co on nc c l lu u s si i o on n: : The re was a cor re la ti on bet we en HSP47 ex pres si on and col la gen ex pres si on in ca ro tid ar te ri es. Ho we ver, in ti mal thic ke ning did not in du ce HSP47 ex pres si on and early col la gen de ve lop ment. The inef fec ti ve nes of TAK-044 sug gests that ET-1 sig na ling may not be imp li ca ted in HSP47 and col la gen in this mo del. K Ke ey y W Wo or rd ds s: : HSP47 he at-shock pro te ins; at he rosc le ro sis; col la gen; en dot he lin-1; rab bits Ö ÖZ ZE ET T A Am ma aç ç: : Eks tra sel lü ler mat riks te ki (ESM) ko la jen ate rosk le roz ve res te no zun ge li şi mi sı ra sın -da vas kü ler ha sa ra ya nı tın mo dü las yo nun da önem li bir rol oy nar. Ko la jen düz kas hüc re si pro li feras yo nu nu, mig ras yo nu nu ve mat riks me tal lop ro te i naz (MMP) üre ti mi ni dü zen le ye bi lir. Bu ne den le ar ter ler de ki ko la jen dön gü sü in ti mal ka lın laş ma nın önem li bir be lir te ci dir. Ko la je ne öz gü mo le kü -ler bir şape ron olan ısı şoku pro te i ni 47'nin (HSP47) pro ko la jen mo le kül le ri nin iş len me si ve sek resyo nu için te mel ol du ğu dü şü nül mek te dir. En do t...

Assessment of serum MMP-9, TIMP-1 levels and MMP-9/TIMP-1 ratio in migraine patients with and without aura

“…Matrix metalloproteinases (MMP) are the enzymes catalyzing the degrading of extracellular matrix components. They are synthesized by several cell groups, mainly macrophages and lymphocytes 7 . Their functions and roles in the pathophysiology of many disorders, such as cancers, arthritis, infections, cardiovascular and neurological diseases have been studied since the 1960s 8 .…”