Endothelial Survival in Corneal Tissue Stored in M-K Medium

Horn, Diane L. Van; Schultz, Richard O.; DeBruin, Janet

doi:10.1016/0002-9394(75)90395-5

Cited by 45 publications

(13 citation statements)

References 2 publications

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“…But, according to the study of Van Horn, stored cat corneas were found to have a marked increase in trypan blue vital staining and cellular disruption of endothelium, if corneas were incubated for two h at 35 Њ C in fresh tissue culture medium before evaluation (40). In this present study, the corneas were warmed up to room temperature for two h before evaluation; therefore, it is suspected that the increased cellular disruption found, as compared to that in other studies, was an effect of temperature.…”

Section: Discussionmentioning

confidence: 99%

Effect of shaking on corneal endothelial preservation

Wang

1997

Current Eye Research

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Section: Discussionmentioning

confidence: 99%

Effect of shaking on corneal endothelial preservation

Wang

1997

Current Eye Research

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“…Preservation of corneoscleral segments in MK-medium was first reported by McCarey and Kaufman [1974], They demonstrated that rabbit corneal endothelium remained ultrastructurally and functionally intact up to 14 days when stored in M K-medium [ Van Horn et al, 1975;Breslin et al, 1976], Further laboratory studies confirmed the viability of human endothelium stored in MK-medium for at least 4 days [Holtmann et al, 1976;Van Horn et al, 1975;McCarey, 1977], In order to find out the effect of pre servation techniques on keratoplasty, a comparison of the visual acuity in cases with keratoconus (good prognosis) and cases with corneal opacity with vascularization (bad prognosis) was done. The comparison of the two independent mean values is shown in table X.…”

Section: Resultsmentioning

confidence: 99%

Critical Evaluation of Corneal Graft Surgery Using Fresh Corneas and Corneas Preserved in MK-Medium

Sayegh¹

1982

Ophthalmologica

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Penetrating keratoplasty was done in 112 patients. Two types of donor material were used: whole eyes preserved in a moist chamber at 4°C, and corneoscleral segments preserved in MK-medium 4°C. Comparison of the postoperative results was done. No significant correlation was found between the postoperative results and the difference between donor and recipient age. The subjective evaluation of donor material was not accurate. The death-to-enucleation time of 6.1 ± 6.2h was tolerated and had no significant influence on the postoperative result. A partial relationship was found between postoperative vision and death-to-surgery time. The earlier the surgery, the better were the results. Both preservation methods, whole eyes in a moist chamber and corneoscleral segments in MK-medium, were useful in cases with good prognosis, e.g. keratoconus. The MK-preservation method was found to be superior in cases with corneal opacity and vascularization.

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“…This does not mean that the warming caused the disruption; rather, the warming allowed the nonviable endothelial cells to become manifest. 3 Warming the tissue to 36°C mimics the change in temperature that occurs with cor¬ neal transplantation. Because the tis¬ sue remains in preservative medium rather than in a recipient eye during warming, our method is not complete¬ ly analogous to the clinical situation.…”

Section: Resultsmentioning

confidence: 99%

“…3 We investigated the effects of warming before fixation by storing two corneal pairs in K-Sol and two in CSM for two weeks at 4°C. One cornea was warmed to 36°C for two hours, and the fellow cornea was kept at room temperature for two hours before fixation.…”

Section: Methodsmentioning

confidence: 99%