Critical Evaluation of Corneal Graft Surgery Using Fresh Corneas and Corneas Preserved in MK-Medium

Abstract

Penetrating keratoplasty was done in 112 patients. Two types of donor material were used: whole eyes preserved in a moist chamber at 4°C, and corneoscleral segments preserved in MK-medium 4°C. Comparison of the postoperative results was done. No significant correlation was found between the postoperative results and the difference between donor and recipient age. The subjective evaluation of donor material was not accurate. The death-to-enucleation time of 6.1 ± 6.2h was tolerated and had no significant influence on the postoperative result. A partial relationship was found between postoperative vision and death-to-surgery time. The earlier the surgery, the better were the results. Both preservation methods, whole eyes in a moist chamber and corneoscleral segments in MK-medium, were useful in cases with good prognosis, e.g. keratoconus. The MK-preservation method was found to be superior in cases with corneal opacity and vascularization.

Evaluation of penetrating keratoplasty in keratoconus

“…The presented data confirms a previous study by Sayegh (1982) that the different preservation methods are useful in cases with good prognosis, e.g., keratoconus. The role of MK-medium and tissue culture in cases with vascularized corneal opacity will be the subject of another study.…”

Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31P-Magnetic Resonance Analysis

“…1964;Hoeffle, 1969;Sherrard, 1973;McCarcy andKaufman, 1974: McCarey, 1976;Friedland and Forster, 1976;Bin der, 1980] and excised cornea in McCareyKaufman (M-K) medium [McCarey and Kaufman, 1974;McCarey, 1976;McCarey et al, , 1976Stark et al, 1975;Van Horn et al, 1975;Breslin et al, 1976;Friedland and Forster, 1976;Aquavella et al, 1976;Binder and Wickham, 1978;Binder, 1980;Sayegh, 1982;Harbour and Stern, 1983;Neubauer et al, 1984] at 4°C have suggested that the maximum storage time for each technique is 24-48 h and 96 h respec tively. Although clinical experience within these limits has been successful with each of these storage methods, there remains little and conflicting information on the meta bolic status of the cornea and on the dura tion of corneal viability.…”